Thermoresponsive anionic copolymer brush-grafted surfaces for cell separation

Nagase, Kenichi; Uchikawa, Naho; Hirotani, Tadashi; Akimoto, Aya Mizutani; Kanazawa, Hideko

doi:10.1016/j.colsurfb.2019.110565

Cited by 39 publications

(18 citation statements)

References 51 publications

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“…[ 34 ] In addition, acrylic acid (AAc) has been incorporated into PNIPAAm through random polymerization using surface‐initiated ATRP. [ 35 ] Using thermoresponsive anionic copolymer‐modified substrates, the separation of endothelial cells and smooth muscle cells was able to be performed because the thermoresponsive anionic copolymers had selectivity for smooth muscle cells. [ 35 ] These investigations used random ionic copolymer brushes.…”

Section: Figurementioning

confidence: 99%

“…[ 35 ] Using thermoresponsive anionic copolymer‐modified substrates, the separation of endothelial cells and smooth muscle cells was able to be performed because the thermoresponsive anionic copolymers had selectivity for smooth muscle cells. [ 35 ] These investigations used random ionic copolymer brushes. In contrast, ATRP can prepare block copolymer brushes through multiple polymerization steps.…”

Section: Figurementioning

confidence: 99%

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Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Nagase

Ota

Hirotani

et al. 2020

Macromol. Rapid Commun.

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Recently, cell separation methods have become important for preparing cells for transplantation therapy. In this study, a thermoresponsive cationic block copolymer brush is developed as an effective cell separation tool. This brush is prepared on glass surfaces using two steps of activator regenerated by electron transfer–atom transfer radical polymerization (ARGET‐ATRP). The cationic segment is prepared in the first step of the ARGET‐ATRP of N,N‐dimethylaminopropylacrylamide (DMAPAAm). In the second step, the thermoresponsive segment is prepared, attached to the bottom cationic segment, through ARGET‐ATRP with N‐isopropylacrylamide (NIPAAm). The cell adhesion behavior of the prepared thermoresponsive cationic copolymer, PDMAPAAm‐b‐PNIPAAm, brush is observed using umbilical cord‐derived mesenchymal stem cells (UCMSC), fibroblasts, and macrophages. At 37 °C, all three types of cells adhere to the thermoresponsive cationic copolymer brush. Then, by reducing the temperature to 20 °C, the adhered UCMSC are detached from the copolymer brush, whereas the fibroblasts and macrophages remain adhered to the copolymer brush. Using this copolymer brush, UCMSC can be purified from the cell mixture simply by changing the temperature. Therefore, the prepared thermoresponsive cationic copolymer brush is useful as a cell separation tool for the purification of mesenchymal stem cells.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Nagase

Ota

Hirotani

et al. 2020

Macromol. Rapid Commun.

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“…67 To improve selectivity, an ionic PNIPAAm copolymer was used; the differences in ionic properties among cell types enable specific cell adhesion on PNIPAAm copolymermodified substrates. 26,[68][69][70] Using this approach, mesenchymal stem cells have been effectively separated. 26,70 In addition, using laminin-coated PNIPAAm modified dishes, cardiomyocytes differentiated from human pluripotent stem cells (hPSCs) were purified from undifferentiated hPSCs because cardiomyocytes selectively detached from the dish because of the relatively weaker adhesive properties compared to those of undifferentiated hPSCs, whereas undifferentiated hPSCs remained on the culture dish because of the relatively stronger adhesive properties compared with those of cardiomyocytes.…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive block copolymer brush for temperature-modulated hepatocyte separation

et al. 2022

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“…Stimuli-responsive polymers, whose physical and chemical properties can vary in response to external stimuli including heat, 1-3 light, [4][5][6][7] and pH, [8][9][10] have attracted much attention for their broad applications 11 in elds such as cell surface manipulation, [12][13][14] nano drivers, 15,16 and biomolecular separation. 17,18 Based on the density of the chains and the interactions between the chain and the interface, stimulus-responsive polymers can be categorized into single-chain polymers, multi-layer polymer components, and polymer brushes.…”

Section: Introductionmentioning

confidence: 99%