Thermoresponsive Bacterial Cellulose Whisker/Poly(NIPAM-<i>co</i>-BMA) Nanogel Complexes: Synthesis, Characterization, and Biological Evaluation

Wu, Lei; Zhou, Hui; Sun, Hokeun; Zhao, Yufang; Yang, Xiangliang; Cheng, Stephen Z. D.; Yang, Guang

doi:10.1021/bm3019664

Cited by 54 publications

(22 citation statements)

References 39 publications

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“…20 Five replicate measurements were obtained for each sample. The medium in each 96-well plate was then removed completely and 120 mL of MTT (Beyotime, China) mixed solution was added to each culture well, which consisted of 100 mL of culture medium and 20 mL of MTT solution (0.5 mg mL À1 ).…”

Section: Cell Evaluationmentioning

confidence: 99%

Bacterial cellulose–hyaluronan nanocomposite biomaterials as wound dressings for severe skin injury repair

et al. 2015

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Bacterial cellulose (BC), a network of pure cellulose nanofibers with fine crystallinity, high mechanical strength and wet capability, and good biocompatibility, is a good material candidate for wound dressing. Hyaluronan (HA) has obvious curative properties, promoting the healing of wound skin tissue and reducing scar formation. This study explored an ''orifice plate'' culture method to obtain BC samples of different sizes but consistent thicknesses. Novel BC-HA nanocomposites with a 3-D network structure were obtained through a solution impregnation method. The total surface area and the pore volume of the BC-HA composite films gradually decreased with the increase of HA content. The elongation of BC-HA composite films at the break point gradually increased as the HA content increased while the tensile strength of the BC-HA composite films decreased during the same process. The BC-HA composite films had a better water uptake capability than pure BC, and water vapor transmission rate (WVTR) measurements showed that the BC-HA composite films can satisfy breathing requirements of injured skin. The BC-HA composite films facilitated the growth of primary human fibroblast cells, showing their low toxicity, and the BC-HA composite films with 0.1% HA lead to higher levels of cell viability than the pure BC. In vivo experiments indicated that the BC-HA with 0.1% HA had the shortest wound healing time while BC-HA with 0.05% HA yielded best tissue repair results. The BC-HA composite films are expected to be useful as novel wound dressing materials for clinical skin repair.

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Section: Cell Evaluationmentioning

confidence: 99%

Bacterial cellulose–hyaluronan nanocomposite biomaterials as wound dressings for severe skin injury repair

et al. 2015

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“…In another example, Wu et al . reported nanogel complexes made of poly( N ‐isopropyl acrylamide‐ co ‐butyl methacrylate) (PNB) and different contents of BNC with a surfactant‐free emulsion polymerization [Figure (C)].…”

Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 99%

“…This led to the formation of opaque shrunken nanogels. These nanogel complexes may be an ideal candidate for a wide range of medical applications, such as injectable biomaterials and vascular embolization interventional therapies …”

Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 99%

Recent advances in nanocellulose for biomedical applications

Jorfi

Foster

2014

J of Applied Polymer Sci

657

368

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Nanocellulose materials have undergone rapid development in recent years as promising biomedical materials because of their excellent physical and biological properties, in particular their biocompatibility, biodegradability, and low cytotoxicity. Recently, a significant amount of research has been directed toward the fabrication of advanced cellulose nanofibers with different morphologies and functional properties. These nanocellulose fibers are widely applied in medical implants, tissue engineering, drug delivery, wound-healing, cardiovascular applications, and other medical applications. In this review, we reflect on recent advancements in the design and fabrication of advanced nanocellulose-based biomaterials (cellulose nanocrystals, bacterial nanocellulose, and cellulose nanofibrils) that are promising for biomedical applications and discuss material requirements for each application, along with the challenges that the materials might face. Finally, we give an overview on future directions of nanocellulose-based materials in the biomedical field.

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“…Hence, BNC-based antibacterial composites are usually fabricated. Numerous research groups combined drug, 12 polymers, 13 and nanoparticles 13 in BNC to fabricate wound dressing composites with antibacterial capability, but the result was not so favorable as either they applied toxic materials in synthesis process or few successful results have been reported. In most studies chemical methods have been applied to prepare Ag-NPs, which restrict the medical uses of nanocomposites, due to the toxicity of starting materials.…”

Section: Introductionmentioning

confidence: 99%

In vitro molecular study of wound healing using biosynthesized bacteria nanocellulose/silver nanocomposite assisted by bioinformatics databases

Moniri¹,

Moghaddam²,

Azizi³

et al. 2018

IJN

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BackgroundIn recent years, bacterial nanocellulose (BNC) based nanocomposites have been developed to promote healing property and antibacterial activity of BNC wound dressing. Molecular study can help to better understanding about interaction of genes and pathways involved in healing progression.ObjectivesThe aim of this study was to prepare bacterial nanocellulose/silver (BNC/Ag) nanocomposite films as ecofriendly wound dressing in order to assess their physical, cytotoxicity and antimicrobial properties. The in vitro molecular study was performed to evaluate expression of genes involved in healing of wounds after treatment with BNC/Ag biofilms.Study design, materials, and methodsSilver nanoparticles were formed by using Citrullus colocynthis extract within new isolated bacterial nanocellulose (BNC) RM1. The nanocomposites were characterized using X-ray diffraction, Fourier transform infrared, and field emission scanning electron microscopy. Besides, swelling property and Ag release profile of the nanocomposites were studied. The ability of nanocomposites to promote wound healing of human dermal fibroblast cells in vitro was studied. Bioinformatics databases were used to identify genes with important healing effect. Key genes which interfered with healing were studied by quantitative real time PCR.ResultsSpherical silver nanoparticles with particle size ranging from 20 to 50 nm were synthesized and impregnated within the structure of BNC. The resulting nanocomposites showed significant antibacterial activities with inhibition zones ranging from 7±0.25 to 16.24±0.09 mm against skin pathogenic bacteria. Moreover, it was compatible with human fibroblast cells (HDF) and could promote in vitro wound healing after 48h. Based on bioinformatics databases, the genes of TGF-β1, MMP2, MMP9, CTNNB1, Wnt4, hsa-miR-29b-3p and hsa-miR-29c-3p played important role in wound healing. The nanocomposites had an effect in expression of the genes in healing. Thus, the BNC/Ag nanocomposite can be used to heal wound in a short period and simple manner.ConclusionThis eco-friendly nanocomposite with excellent antibacterial activities and healing property confirming its utility as potential wound dressings.

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Thermoresponsive Bacterial Cellulose Whisker/Poly(NIPAM-co-BMA) Nanogel Complexes: Synthesis, Characterization, and Biological Evaluation

Cited by 54 publications

References 39 publications

Bacterial cellulose–hyaluronan nanocomposite biomaterials as wound dressings for severe skin injury repair

Bacterial cellulose–hyaluronan nanocomposite biomaterials as wound dressings for severe skin injury repair

Recent advances in nanocellulose for biomedical applications

In vitro molecular study of wound healing using biosynthesized bacteria nanocellulose/silver nanocomposite assisted by bioinformatics databases

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