Thermoresponsive Cellulosic Hydrogels with Cell-Releasing Behavior

Hoo, Siew Pei; Sarvi, Fatemeh; Li, Wai Ho; Chan, Peggy; Yue, Zhilian

doi:10.1021/am4009133

Cited by 28 publications

(17 citation statements)

References 48 publications

(66 reference statements)

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“…34 Hydroxypropyl cellulose is a natural cellulose approved by FDA and has good biodegradability and compatibility. 35 Glycerol is commonly used as an additive in medicine and has been proven to be biocompatible in vivo. 36 For example, Cardoso et al added glycerol as a component of the hydrogel in the preparation of calcium alginate gel, which proved that it had good biocompatibility in vivo.…”

Section: Discussionmentioning

confidence: 99%

<p>Antitumor Activity of Thermosensitive Hydrogels Packaging Gambogic Acid Nanoparticles and Tumor-Penetrating Peptide iRGD Against Gastric Cancer</p>

Zhang¹,

Chu²,

Hu³

et al. 2020

IJN

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Introduction: Gambogic acid (GA) is proved to have anti-tumor effects on gastric cancer. Due to poor solubility, non-specific biological distribution, toxicity to normal tissues and short half-life, it is hard to be applied into the clinic. To overcome these issues, we developed a thermosensitive and injectable hydrogel composed of hydroxypropyl cellulose, silk fibroin and glycerol, with short gelling time, good compatibility and sustained release, and demonstrated that the hydrogel packaged with gambogic acid nanoparticles (GA-NPs) and tumorpenetrating peptide iRGD could improve the anti-tumor activity. Methods: The Gelling time and micropore size of the hydrogels were regulated through different concentrations of glycerol. Controlled release characteristics of the hydrogels were evaluated with a real-time near-infrared fluorescence imaging system. Location of nanoparticles from different carriers was traced by confocal laser scanning microscopy. The in vivo antitumor activity of the hydrogels packaging GA-NPs and iRGD was evaluated by investigating tumor volume and tumor size. Results: The thermo-sensitive properties of hydrogels were characterized by 3-4 min, 37°C, when glycerol concentration was 20%. The hydrogels physically packaged with GA-NPs and iRGD showed higher fluorescence intensity than other groups. The in vivo study indicated that the co-administration of GA-NPs and iRGD by hydrogels had higher antitumor activity than the GA-loaded hydrogels and free GA combining with iRGD. Free GA group showed few antitumor effects. Compared with the control group, the body weight in other groups had no obvious change, and the count of leukocytes and hemoglobin was slightly decreased. Discussion: The hydrogel constructed iRGD and GA-NPs exerted an effective anti-tumor effect possibly due to retention effect, local administration and continuous sustained release of iRGD promoting the penetration of nanoparticles into a deep part of tumors. The delivery system showed little systemic toxicity and would provide a promising strategy to improve anti-gastric cancer efficacy.

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Section: Discussionmentioning

confidence: 99%

<p>Antitumor Activity of Thermosensitive Hydrogels Packaging Gambogic Acid Nanoparticles and Tumor-Penetrating Peptide iRGD Against Gastric Cancer</p>

Zhang¹,

Chu²,

Hu³

et al. 2020

IJN

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“…However, interests in developing NO-releasing particles keep increasing. The NO-releasing particles are not only able to impose controlling effects on the NO liberation by themselves but could also be conveniently synchronized into a variety of 3D materials including hydrogels, [101] microcapsules, [102] and electrospunfibers, [103,104] which were used for drug delivery and cell encapsulation without losing their bioactivities, for the enhancement in spatiotemporal control of NO delivery. The NO release profiles of 3D materials could be tailored by varying the amount of NO-releasing particles incorporated in order to meet the requirements for specific applications.…”

Section: Polymeric Particlesmentioning

confidence: 99%

Modulated nitric oxide delivery in three-dimensional biomaterials for vascular functionality

et al. 2017

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Nitric oxide (NO) acts a pivotal role in regulating various physiological processes of vasodilation, platelet aggregation, and vascular smooth muscle cell mitogenesis and proliferation. This makes NO a promising candidate for the treatment of cardiovascular problems like hypertension and vascular stenosis. However, the high reactivity of NO poses an issue for effective NO delivery. To overcome this limitation, recent developments on three-dimensional (3D) materials have been explored with either physical or chemical incorporation of NO releasing donors, to provide spatiotemporal control over NO-signaling pathways in blood vessels. Here, we offer an overview on the current efforts, and propose future perspectives for precise regulation on NO delivery in advanced 3D materials toward proper vascular functionality.

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“…UV irradiation on photo initiators combined with hydrogels can cause phase transition and be used to encapsulate cells [ 42 , 43 ]. However, long-term irradiation of UV can affect the viability of cells encapsulated on the glass slide [ 43 , 44 ]. Covalent crosslinking by radical reactions can be employed for microarray bioprinting as well.…”

Section: Factors To Be Considered When Selecting Hydrogels For Micmentioning

confidence: 99%

Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation

2015

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Conventional drug screening processes are a time-consuming and expensive endeavor, but highly rewarding when they are successful. To identify promising lead compounds, millions of compounds are traditionally screened against therapeutic targets on human cells grown on the surface of 96-wells. These two-dimensional (2D) cell monolayers are physiologically irrelevant, thus, often providing false-positive or false-negative results, when compared to cells grown in three-dimensional (3D) structures such as hydrogel droplets. However, 3D cell culture systems are not easily amenable to high-throughput screening (HTS), thus inherently low throughput, and requiring relatively large volume for cell-based assays. In addition, it is difficult to control cellular microenvironments and hard to obtain reliable cell images due to focus position and transparency issues. To overcome these problems, miniaturized 3D cell cultures in hydrogels were developed via cell printing techniques where cell spots in hydrogels can be arrayed on the surface of glass slides or plastic chips by microarray spotters and cultured in growth media to form cells encapsulated 3D droplets for various cell-based assays. These approaches can dramatically reduce assay volume, provide accurate control over cellular microenvironments, and allow us to obtain clear 3D cell images for high-content imaging (HCI). In this review, several hydrogels that are compatible to microarray printing robots are discussed for miniaturized 3D cell cultures.

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Thermoresponsive Cellulosic Hydrogels with Cell-Releasing Behavior

Cited by 28 publications

References 48 publications

<p>Antitumor Activity of Thermosensitive Hydrogels Packaging Gambogic Acid Nanoparticles and Tumor-Penetrating Peptide iRGD Against Gastric Cancer</p>

<p>Antitumor Activity of Thermosensitive Hydrogels Packaging Gambogic Acid Nanoparticles and Tumor-Penetrating Peptide iRGD Against Gastric Cancer</p>

Modulated nitric oxide delivery in three-dimensional biomaterials for vascular functionality

Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation

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