Thermospray liquid chromatographic/mass spectrometric studies with inositol phosphates

Hsu, Fong‐Fu; Goldman, H. D.; Sherman, William R.

doi:10.1002/bms.1200191004

Cited by 13 publications

(8 citation statements)

References 6 publications

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“…Hsu et al [434] applied a thermospray liquid chromatographic/mass spectrometric combination for the determination of inositol phosphates. They separated inositol phosphates (InsP 1 , InsP 3 , InsP 6 ) by anion-exchange chromatography on Mono-Q with ammonium formate, heated at 2608C (pH 4) and determined mass spectrometrically the dephosphorylated inositol moiety as m/z ion 198 [NH 4 N inositol] + .…”

Section: Mass Spectrometric Detectionmentioning

confidence: 99%

Phytate in foods and significance for humans: Food sources, intake, processing, bioavailability, protective role and analysis

Schlemmer¹,

Frølich

Prieto

et al. 2009

Molecular Nutrition Food Res

746

604

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The article gives an overview of phytic acid in food and of its significance for human nutrition. It summarises phytate sources in foods and discusses problems of phytic acid/phytate contents of food tables. Data on phytic acid intake are evaluated and daily phytic acid intake depending on food habits is assessed. Degradation of phytate during gastro-intestinal passage is summarised, the mechanism of phytate interacting with minerals and trace elements in the gastro-intestinal chyme described and the pathway of inositol phosphate hydrolysis in the gut presented. The present knowledge of phytate absorption is summarised and discussed. Effects of phytate on mineral and trace element bioavailability are reported and phytate degradation during processing and storage is described. Beneficial activities of dietary phytate such as its effects on calcification and kidney stone formation and on lowering blood glucose and lipids are reported. The antioxidative property of phytic acid and its potentional anticancerogenic activities are briefly surveyed. Development of the analysis of phytic acid and other inositol phosphates is described, problems of inositol phosphate determination and detection discussed and the need for standardisation of phytic acid analysis in foods argued.

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Section: Mass Spectrometric Detectionmentioning

confidence: 99%

Phytate in foods and significance for humans: Food sources, intake, processing, bioavailability, protective role and analysis

Schlemmer¹,

Frølich

Prieto

et al. 2009

Molecular Nutrition Food Res

746

604

View full text Add to dashboard Cite

show abstract

“…B (2002) phosphates in biological matter, but not environmental samples per se, possibly due to the high detection limit of ca. 190 g P l Ϫ1 for the LC-MS technique (Hsu et al 1990;Shang et al 1992). This situation seems unlikely to change while there is a preference for cheaper, more versatile instrumentation and, potentially, on-line monitoring of inositol phosphates.…”

Section: (D ) Spectroscopic Detection Techniques (I) Nuclear Magneticmentioning

confidence: 99%

Inositol phosphates in the environment

Turner¹,

Paphazy

Haygarth³

et al. 2002

Phil. Trans. R. Soc. Lond. B

684

554

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The inositol phosphates are a group of organic phosphorus compounds found widely in the natural environment, but that represent the greatest gap in our understanding of the global phosphorus cycle. They exist as inositols in various states of phosphorylation (bound to between one and six phosphate groups) and isomeric forms (e.g. myo, d-chiro, scyllo, neo), although myo-inositol hexakisphosphate is by far the most prevalent form in nature. In terrestrial environments, inositol phosphates are principally derived from plants and accumulate in soils to become the dominant class of organic phosphorus compounds. Inositol phosphates are also present in large amounts in aquatic environments, where they may contribute to eutrophication. Despite the prevalence of inositol phosphates in the environment, their cycling, mobility and bioavailability are poorly understood. This is largely related to analytical difficulties associated with the extraction, separation and detection of inositol phosphates in environmental samples. This review summarizes the current knowledge of inositol phosphates in the environment and the analytical techniques currently available for their detection in environmental samples. Recent advances in technology, such as the development of suitable chromatographic and capillary electrophoresis separation techniques, should help to elucidate some of the more pertinent questions regarding inositol phosphates in the natural environment.

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“…Mass spectrometry (MS) with soft ionization methods, such as fast atom bombardment (FAB) and thermospray, allows the analysis of inositol phosphates without derivatization 20, 21. The application of FAB and collisional‐activated dissociation (CAD) with mass analyzed ion kinetic energy spectrometry (MIKES) has been used in attempts to differentiate isomers of inositol mono‐, bis‐ and trisphosphates 22.…”

Section: Introductionmentioning

confidence: 99%

Structural distinction among inositol phosphate isomers using high‐energy and low‐energy collisional‐activated dissociation tandem mass spectrometry with electrospray ionization

2003

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Electrospray (ESI) collisional-activated dissociation (CAD) tandem mass spectrometric methods for the structural characterization of inositol phosphates (InsPs) using both quadrupole and sector mass spectrometers are described. Under low-energy CAD, the [M + H](+) ions of the positional isomers of inositol phosphates, including inositol mono-, bis- and trisphosphates, yield distinguishable product-ion spectra, which are readily applicable for isomer differentiation. In contrast, the product-ion spectra arising from high-energy CAD (2 keV collision energy, floating at 50%) tandem sector mass spectrometry are less applicable for isomer identification. The differences in the product-ion spectrum profiles among the aforementioned InsP isomers become more substantial and differentiation of positional isomers can be achieved when the collison energy is reduced to 1 keV (floating at 75%). These results demonstrate that the applied collision energies play a pivotal role in the fragmentations upon CAD. The product-ion spectra are similar among the positional isomers of inositol tetrakisphosphates and of inositol pentakisphosphates. Thus, isomeric distinction for these two inositol polyphosphate classes could not be established by the tandem mass spectrometric methods that have achieved such distinctions for the less highly phosphorylated inositol phosphate classes. Under both high- and low-energy CAD, the protonated molecular species of all InsPs undergo similar fragmentation pathways, which are dominated by the consecutive losses of H(2)O, HPO(3) and H(3)PO(4).

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Thermospray liquid chromatographic/mass spectrometric studies with inositol phosphates

Cited by 13 publications

References 6 publications

Phytate in foods and significance for humans: Food sources, intake, processing, bioavailability, protective role and analysis

Phytate in foods and significance for humans: Food sources, intake, processing, bioavailability, protective role and analysis

Inositol phosphates in the environment

Structural distinction among inositol phosphate isomers using high‐energy and low‐energy collisional‐activated dissociation tandem mass spectrometry with electrospray ionization

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