Thermostabilisation du vaccin antiamaril 17-d lyophilisé. II. Lots-pilotes préparés dans les conditions d'une production industrielle

Barme, M; Vacher, Bernard; Ryhiner, Marie-Louise; Chabannier, G.

doi:10.1016/0092-1157(87)90017-5

Cited by 3 publications

(1 citation statement)

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“…Role of heavy water (D 2 O) in biological sciences especially in thermostabilization of vaccines has been thoroughly reviewed [6]. The first experimental evidence of D 2 O mediated thermostabilization was demonstrated in yellow fever 17D vaccines [7]. Later, the potential of D 2 O was extensively studied for thermostabilization of oral polio virus vaccine [8–11].…”

Section: Introductionmentioning

confidence: 99%

Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Singha

Goyal

Malik

et al. 2014

The Scientific World Journal

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Thermostabilizing effect of heavy water (D2O) or deuterium oxide has been demonstrated previously on several enzymes and vaccines like oral poliovirus vaccine and influenza virus vaccine. In view of the above observations, effect of heavy water on in situ thermostabilization of recombinant p26 protein on enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of equine infectious anemia virus (EIAV) infection was investigated in the present study. The carbonate-bicarbonate coating buffer was prepared in 60% and 80% D2O for coating the p26 protein in 96-well ELISA plate and thermal stability was examined at 4°C, 37°C, 42°C, and 45°C over a storage time from 2 weeks to 10 months. A set of positive serum (n = 12) consisting of strong, medium, and weak titer strength (4 samples in each category) and negative serum (n = 30) were assessed in ELISA during the study period. At each time point, ELISA results were compared with fresh plate to assess thermal protective effect of D2O. Gradual increase in the stabilizing effect of 80% D2O at elevated temperature (37°C < 42°C < 45°C) was observed. The 80% D2O provides the thermal protection to rp26 protein in ELISA plate up to 2 months of incubation at 45°C. The findings of the present study have the future implication of adopting cost effective strategies for generating more heat tolerable ELISA reagents with extended shelf life.

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Section: Introductionmentioning

confidence: 99%

Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Singha

Goyal

Malik

et al. 2014

The Scientific World Journal

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Stability-related studies on 17D yellow fever vaccine

Perraut¹,

Girault²,

Moreau³

2000

Microbes and Infection

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Thermostability of vaccines

Gałazka¹,

Milstien²,

Zaffran³

1999

Rev Panam Salud Publica

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Thermostabilisation du vaccin antiamaril 17-d lyophilisé. II. Lots-pilotes préparés dans les conditions d'une production industrielle

Cited by 3 publications

References 2 publications

Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Stability-related studies on 17D yellow fever vaccine

Thermostability of vaccines

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Thermostabilisation du vaccin antiamaril 17-d lyophilisé. II. Lots-pilotes préparés dans les conditions d'une production industrielle

Cited by 3 publications

References 2 publications

Use of Heavy Water (D2O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Use of Heavy Water (D2O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Stability-related studies on 17D yellow fever vaccine

Thermostability of vaccines

Contact Info

Product

Resources

About

Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection