Thermostability, photostability, and toxicity of clove oil nanoparticles against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae)

Abstract

Abstract. Ikawati S, Himawan T, Abadi AL, Tarno H. 2020. Thermostability, photostability, and toxicity of clove oil nanoparticles against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae). Biodiversitas 21: 4764-4771. Problems related to the essential oils as insecticides are their volatility, poor solubility in water and environmental degradation (such as caused by high temperatures and UV light) which can adversely affect the application. An ideal botanical insecticide formulation would maintain the level of the main compounds adequate for pest control. This study was to evaluate the stability of polyethylene glycol (PEG) coated nanoparticles loaded with clove (Syzygium aromaticum) essential oil to high temperature and UV light also their toxicity to C. ferrugineus. There is a reduction in nanoparticle size after heating from 257 to 173 nm and there is no difference in size distribution. After heating and UV-light treatment, eugenol content in free clove oil decreased significantly, while on nanoparticles there were not significantly changed, that indicated greater stability on the nanoformulation. The median lethal concentration (LC50) of CO-NPs to C. ferrugineus adults value for 72 and 120 hours was 0.734 and 0.676%.

Toxicity nanoinsecticide based on clove essential oil against <i>Tribolium castaneum</i> (Herbst)

“…During the preparation process of visualizing an image on the nanoparticles, the heat generated by electron beams could be caused by the melting of PEG, which may have softened and melted the PEG wall. 17) The size average of nanoparticles also different from the previous result, 23) which in this study was better because smaller. This is probably because during the grinding process, in this study it was more optimal than the previous study, such as the duration of the grinding process is optimal.…”

“…This result is the same as the previous result that also showed clove oil nanoparticles size distribution is bimodal. 23) Analysis of the results from GC-MS indicates there were no significant chemical variations between pre-encapsulated and post-encapsulated, with a composition of two main components, namely eugenol and trans-caryophyllene (Table 2). This means, there was no oxidized found, except in the variations of the content ratio of major constituents.…”

Antibiofilm Activity of Eugenol-Loaded Chitosan Coatings against Common Medical-Device-Contaminating Bacteria

“…For example, a coating from chitosan, poly(vinyl alcohol), and PEO-loaded MSNs developed by Jia et al released 16.4%, 26.8%, and 46.5% after 1, 2, and 5 days in PBS. The aggregation of MSN-EUG, entrapment of the particles in the chitosan matrix, lack of exterior or interior pores, smaller MSN pore size, and differences in the media composition could have contributed to the lower release observed in the current study than in previous reports. ,, This work also reported the natural loss of eugenol that could have impacted its concentration in the release medium over time, which has been reported in previous studies. , This might need to be taken into consideration by future release studies.…”

“…21,22,51 This work also reported the natural loss of eugenol that could have impacted its concentration in the release medium over time, which has been reported in previous studies. 52,53 This might need to be taken into consideration by future release studies. GA0.5%-CHI showed significant growth inhibition of planktonic MRSA and P. aeruginosa but not S. aureus.…”

In Silico Study of Eugenol and trans-Caryophyllene also Clove Oil Fumigant Toxicity on Tribolium castaneum

“…The content of eugenol and trans-caryophyllene in this study is following the results of previous studies. Eugenol (85.39%) and transcaryophyllene (9.22%) were identified as two major components of clove oil [31]. clove oil and its main effective composition eugenol show many beneficial advantages [32].…”