Thermostable alkaline protease from Thermomyces lanuginosus: optimization, purification and characterization

Ghareib, Mohamed; Fawzi, Eman M.; Aldossary, Nouf A.

doi:10.1007/s13213-013-0725-7

Cited by 14 publications

(3 citation statements)

References 26 publications

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“…Also, the enzyme was slightly inactivated by iodoacetate a thiol reducing agent and EDTA. These results were in complete accordance with that recorded for Thermomyces lanuginosus protease 49 .…”

Section: Discussionsupporting

confidence: 91%

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

2020

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Introduction Protease enzymes EC 3.4.21-24 are fit for hydrolyzing the bonds between amino acids of protein molecule and they are the major industrial enzymes, which represented 60 of the complete synthetically produced enzymes 1. Alkaline proteases of microbial origin have impressive industrial intensity because of their biochemical decent variety and enormous applications in detergents, pharmaceutical, nourishment, dairy and leather industries 2. Fungi are the most microbes producing proteases with many advantages, since they are normally GRAS generally regarded as safe strains and their enzymes are secreted outside the cell which easily recover the enzyme 3, 4. Despite the fact that fungi are significant wellsprings of alkaline proteases 5, 6 , studies on their protease production are confined for the reason that the fungi prefer acidic and neutral pH values. Thus, it is of prime interest to search for

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Section: Discussionsupporting

confidence: 91%

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

2020

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“…Both options seem more advantageous in fungi than other protease-producing organisms (microbial or not) [ 103 , 163 ]. Among the protease-producing fungi, thermophilic fungi such as Thermoascus aurantiacus [ 164 ] or Thermomyces lanuginosus [ 165 ] are of great interest since they possess the ability to secrete thermostable proteases that act in the temperature range 60–85 °C.…”

Section: Significance Of Fungi In Different Sectorsmentioning

confidence: 99%

Current Insights in Fungal Importance—A Comprehensive Review

et al. 2023

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Besides plants and animals, the Fungi kingdom describes several species characterized by various forms and applications. They can be found in all habitats and play an essential role in the excellent functioning of the ecosystem, for example, as decomposers of plant material for the cycling of carbon and nutrients or as symbionts of plants. Furthermore, fungi have been used in many sectors for centuries, from producing food, beverages, and medications. Recently, they have gained significant recognition for protecting the environment, agriculture, and several industrial applications. The current article intends to review the beneficial roles of fungi used for a vast range of applications, such as the production of several enzymes and pigments, applications regarding food and pharmaceutical industries, the environment, and research domains, as well as the negative impacts of fungi (secondary metabolites production, etiological agents of diseases in plants, animals, and humans, as well as deteriogenic agents).

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“…The same moisture level was recorded previously recorded for the production of brinolytic enzyme from Bacillus sp. (Vijayaraghavan et al 2017) However, lower moisture level (71%) was recorded also for proteolytic enzyme production using wheat bran by Aspergillus terreus (Abu-Tahon et al 2020), and higher moisture level 800% was registered as the optimum for maximal production of protease using dates from Thermomyces lanuginosus (Ghareib et al 2014). Lower moisture levels causes a decrease in nutrients solubility of substrates, a higher water tension and lower degree of substrate swelling (Zadražil and Brunnert 1981).…”

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Examination of thrombolytic and anticoagulant activities of purified fibrinolytic enzyme extracted from Cochliobolus hawaiiensis under solid state fermentation

Abu‐Tahon

Abdel-Mageed

Ghareib

et al. 2023

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Cochliobolus hawaiiensis Alcorn AUMC 8606 was chosen from the screened twenty fungal species as the potent producer of fibrinolytic enzyme on skimmed-milk agar plates. The greatest enzyme yield was attained when the submerged fermentation (SmF) conditions were optimized, and it was around (39.7 U/mg protein). Moreover, Upon optimization of fibrinolytic enzyme production under solid state fermentation (SSF), the maximum productivity of fibrinolytic enzyme was greatly increased recorded a bout (405 U/mg protein) on sugar cane bagasse. The yield of fibrinolytic enzyme by C. hawaiiensis under SSF was higher than that of SmF with about 10.20 fold. The purification procedures of fibrinolytic enzyme caused a great increase in its specific activity to 2581.6 U/mg protein with an overall yield of 55.89%, 6.37 purification fold and molecular weight of 35kDa. Maximal activity was recorded at pH 7 and 37oC. The enzyme showed the highest affinity towards Fibrin, with Vmax of 240 U/ml and an apparent Km value of 47.61 mmol. Mg2+ and Ca2+ moderately induced fibrinolytic activity, while Cu2+ and Zn2+ greatly suppressed the enzyme activity. The produced enzyme is categorized as serine protease and non metalloprotease due to the great suppression in its activity by using phenylmethylsulfonyl fluoride and thylenediamine-tetraacetat, respectively. The purified fibrinolytic enzyme showed efficient thrombolytic and antiplaetlet aggregation activities by completely prevention and dissolution of the blood clot which confirmed by microscopic examination and amelioration of blood coagulation assays. These findings suggested that the produced fibrinolytic enzyme is a promising agent in management of blood coagulation disorders

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Thermostable alkaline protease from Thermomyces lanuginosus: optimization, purification and characterization

Cited by 14 publications

References 26 publications

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

Current Insights in Fungal Importance—A Comprehensive Review

Examination of thrombolytic and anticoagulant activities of purified fibrinolytic enzyme extracted from Cochliobolus hawaiiensis under solid state fermentation

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