Thermostable alpha-amylase enzyme production from hot spring isolates Alcaligenes faecalis SSB17 – Statistical optimization

Sen, Sudip Kumar; Dora, D.T.K.; Bandyopadhyay, Bidyut; Mohapatra, Pradeep Kumar Das; Raut, Sangeeta

doi:10.1016/j.bcab.2014.03.005

Cited by 21 publications

(6 citation statements)

References 12 publications

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“…Alpha amylases are industrially produced via submerged fermentation using (often genetically improved) Bacillus and Aspergillus species, but the potential of solid-state culture has been emphasized in the literature [24]. A large spectrum of bacterial and fungal species has been found which produce alpha amylase enzymes possessing different characteristics such as thermos-stability, halo-tolerance, psycho-tolerance and alkali-stability [25,26,27].…”

Section: Industrial Enzymesmentioning

confidence: 99%

A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

Ravindran¹,

Hassan²,

Williams³

et al. 2018

Bioengineering

226

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Agro-industrial waste is highly nutritious in nature and facilitates microbial growth. Most agricultural wastes are lignocellulosic in nature; a large fraction of it is composed of carbohydrates. Agricultural residues can thus be used for the production of various value-added products, such as industrially important enzymes. Agro-industrial wastes, such as sugar cane bagasse, corn cob and rice bran, have been widely investigated via different fermentation strategies for the production of enzymes. Solid-state fermentation holds much potential compared with submerged fermentation methods for the utilization of agro-based wastes for enzyme production. This is because the physical–chemical nature of many lignocellulosic substrates naturally lends itself to solid phase culture, and thereby represents a means to reap the acknowledged potential of this fermentation method. Recent studies have shown that pretreatment technologies can greatly enhance enzyme yields by several fold. This article gives an overview of how agricultural waste can be productively harnessed as a raw material for fermentation. Furthermore, a detailed analysis of studies conducted in the production of different commercially important enzymes using lignocellulosic food waste has been provided.

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Section: Industrial Enzymesmentioning

confidence: 99%

A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

Ravindran¹,

Hassan²,

Williams³

et al. 2018

Bioengineering

226

View full text Add to dashboard Cite

show abstract

“…and Aspergillus versicolor isolated from soil by halo zone formed from application of iodine on starch agar plates. Several studies have shown that amylase-producing microbial strains have been isolated from a variety of sources such as water [22], sugar industry's press mud soil [6], hot springs [23]. From garden soil samples, amylase producing B. cereus amy3 was found [10].…”

Section: Discussionmentioning

confidence: 99%

Optimization of B. cereus PW4 alpha amylase production by OVAT technique

Elechi¹,

Jason²,

Akaranta³

et al. 2022

GSC Biol. Pharm. Sci.

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Amylase enzymes are used in a variety of industries. Palm wine was screened for amylase producing microorganisms by starch iodine method. Isolate PW4 was discovered to be the most effective isolate and was identified as Bacillus cereus PW4 (OK384566) by sequencing the 16S rRNA gene. The one-variable-at-a-time strategy was used to optimize α-amylase production parameters, including pH, temperature, incubation duration, inoculum volume, nitrogen source, and metal ion as factors. The enzyme activity was increased to 141.91 U/ml (5.8-fold) in the optimized medium (pH 7, 35 °C, 72 h, 3 % inoculums, 1 % NH4NO3 and 1 % FeCl3) from 24.0 U/ml in the un-optimized medium. Data of the optimal condition will aid in the scaling up of amylase production and other industrial activities.

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“…Amylases are industrially produced via submerged or solid-state fermentation using Bacillus and Aspergillus species [30]. A large spectrum of bacterial and fungal species has been found with the potential to produce amylases of different characteristics such as thermos-stability, halo-tolerance, psycho-tolerance and alkali-stability [31]; [32]. Cellulases, which are enzymes of great commercial importance, especially because of the key role they play in bio-ethanol production similarly derived from Bacillus species and other Fusarium and Geotrichum species in this study was also evident in a study conducted by Singhania et al [33].…”

Section: Acinetobacter Baumanii Bacillus Subtilis B Cereus B Lichenif...mentioning

confidence: 99%

Microbial Profile and Detection of Bio-catalytic Enzymes from Bacterial Derivatives in Selected Agrowastes

Adenikinju

Arotupin

Awojobi

2021

JABB

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The bio-catalytic enzyme potential of microbial consortium in selected agrowastes was evaluated in this study. Bio-wastes possess renewable resources of biomass and represents potentially-valuable sources due to their low cost, environmental friendly and sustainable nature. The isolation, enumeration and biochemistry of bacteria and fungi from agrowastes were conducted via standard microbiological techniques. Screening of agrowaste substrates for enzyme synthesis was conducted via plate assay method. Potato peels had the highest bacterial count of 3.85×104 CFU/ml as maize husk had the highest fungal load of 6.55×104 SFU/ml while wheat shaft had the lowest fungal load of 1.5×104 SFU/ml. Acinetobacter baumanii, Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus subtilis, B. cereus, B. licheniformis, B. megaterium were among bacterial consortium significantly enumerated. Saccharomyces cerevisiae, Rhodotorula glutins, Candida albicans and Geotrichum candidum were yeasts characterized from the fermented agrowastes while Aspergillus niger, A. terreus, A. flavus constitute some of the mould consortium enumerated. Amylase and cellulase content of the agrowaste substrates increased after fermentation while protease and pectinase content of all the agrowastes remained unchanged after the fermentation process. Acinetobacter baumanii, Bacillus subtilis, B. cereus, B. licheniformis, B. megaterium, B. subtilis and G. candidum were positive for amylase, protease, cellulase and pectinase production. Penicillium chrysogenum and Microsporum canis were negative for the enzyme production. The findings of this study demonstrated the enzymatic-production potential of Bacillus species associated with agrowastes because they exhibited higher enzyme production.

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Thermostable alpha-amylase enzyme production from hot spring isolates Alcaligenes faecalis SSB17 – Statistical optimization

Cited by 21 publications

References 12 publications

A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

Optimization of B. cereus PW4 alpha amylase production by OVAT technique

Microbial Profile and Detection of Bio-catalytic Enzymes from Bacterial Derivatives in Selected Agrowastes

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