Thermostable and alkaline-tolerant cellulase-free xylanase produced by thermotolerant Streptomyces sp. Ab106

Techapun, Charin; Charoenrat, Thanakorn; Poosaran, Naiyatat; Watanabe, Masanori; Sasak, Ken

doi:10.1016/s1389-1723(02)80080-9

Cited by 38 publications

(19 citation statements)

References 12 publications

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“…The result of this study is in accordance with that of Techapun et al [50], who reported agitation of 150 rpm as optimal for xylanase production by Streptomyces sp. Ab 106.…”

Section: Effect Of Agitation On Cellulase and Xylanase Productionsupporting

confidence: 92%

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Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

2016

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Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the classical process of one variable per time. Furthermore, the inducing effect of mono-and polysaccharides on enzyme production was investigated. The 16S rDNA gene sequence analysis clearly assigned the isolate to the genus Streptomyces, and was deposited at the GenBank under the accession number KU171373. Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 • C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U/mL and 18.54 U/mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U/g for cellulase and xylanase, respectively. Xylanase production was induced by the entire mono-and polysaccharides tested, while cellulase production was induced by some. The results from this study signify the resourcefulness of the Streptomyces strain for production of cellulase and xylanase of industrial importance.

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“…The result of this study is in accordance with that of Techapun et al [50], who reported agitation of 150 rpm as optimal for xylanase production by Streptomyces sp. Ab 106.…”

Section: Effect Of Agitation On Cellulase and Xylanase Productionsupporting

confidence: 92%

“…MVS3 has been reported [35]. Differences in optimal temperature for enzyme production could be attributed to strain variations between bacterial groups and adaptability to temperature dynamics [50].…”

Section: Effect Of Incubation Temperature On Xylanase and Cellulase Pmentioning

confidence: 99%

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

2016

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“…Many studies have been performed on xylanases from bacteria and fungi (3)(4)(5)(6)(7)(8)(9). Although actinomycetes are an important group of microorganisms involved in lignocellulose degradation in general and xylan in particular, they have been explored to a lesser extent (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

“…Because of the importance of xylanase, there is ongoing interest in the isolation of new bacterial strains producing this enzyme suitable for new industrial applications. There have been reported only a few alkaliphilic or thermophilic xylanases produced by actinomycetes (5,14,15). The rhizospheric actinomycetes became the focus of interest for us (16), due to the fact that they are heterotrophic feeders and play an important role in soil ecology (17).…”

Section: Introductionmentioning

confidence: 99%

A cellulase-free, thermoactive and alkali xylanase production by terrestrial Streptomyces sp. CA24

Porsuk¹

2013

Turk J Biol

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Materials and methods Source of strains and maintenanceSoil samples were previously collected and isolated to determine the composition of cultivable members of the actinomycete community associated with the root system of the local endemic plants Aethionema dumanii, Salvia aytachii, and Achillea ketenoglui. The strains ( 64Streptomyces and 1 Nocardiopsis) used in this study were identified by using molecular tools (16). Spores of strains were maintained as glycerated stocks at 80 °C.

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“…Moreover, data in Figure 4 indicating the role of inducers for effective induction of xylanase, xylan containing substrate such as corn cob PE, phosphatidylethanolamine; DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIMS, phosphatidylinositolmannosides; +, utilized or reaction positive; -, not utilized or reaction negative, R, resist; S, sensitive (23% xylose and 28% xylan) and sugarcane bagasse (28-30% xylan) gave the highest level of xylanase production (1404 and 1241 Ugds -1 ) after the 4th day of fermentation and then declined till day 7 reaching 1299 and 1200 Ugds -1 , respectively. However, Li et al [36] reported that corncobs xylan supported the highest xylanase activity to 334.34 U/ml after 7 days of cultivation in S. chartreusis L1105, Ninawe and Kuhad [3] reported wheat bran and corn cob as an enhancer for xylanase production by Streptomyces cyancus SN32, Techapun et al [37] supported cane bagasse as inducer for cellulase-free xylanase from Streptomyces sp. Ab106 and a significant amounts of xylanase were produced by Aspergillus fumigatus on a variety of agro-wastes but wheat bran supported higher xylanase production followed by rice bran, rice straw and corn cobs as the sole carbon source (8,450; 5,500; 4,600 and 4,500 U/L, respectively) [2].…”

Section: Optimization Of Solid State Fermentation (Ssf) Process Parammentioning

confidence: 99%

Optimization of Solid State Fermentation and Leaching Process Parameters for Improvement Xylanase Production by Endophytic Streptomyces sp. ESRAA-301097

El-Gendy¹

2014

J Microb Biochem Technol

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In the course of our searching program on the microbial endophytes of medical plants (Cympobogon proximus, Anethum graveolens, Artemisia judaica and Corchorus olitorius), the endophytic strain Streptomyces sp. ESRAA-301097 derived from Cympobogon proximus proved to be the hyper xylanase producer. Screening of various locally available agro-industrial residues as substrate support for xylanase production under SSF exhibited a mixture of wheat bran (WB); sugarcane bagasse (SCB) with corncob (CC) at a ratio of 0.5:1:1 as the efficient inducer for the induction of ESRAA-301097 xylanase production as it gave the highest enzyme productivity (2364 Ugds -1 ) at the 4th day of fermentation when compared to individual WB, SCB or CC (1167, 1241 or 1404 Ugds -1 ) after 3, 4 and 4 days of incubation. Xylanase production was enhanced to 3819 Ugds -1 after optimizing the physical process parameters including temperature 30-40°C, pH 7.0, an inoculum level of 10 7 spore gds -1 , 80-85 % initial moisture content and substrate particle size of 800 µm. An overall 23.96 % increase in enzyme production was attained with a mixture of soybean and corn steep solid as a nitrogen source but no enhancement was obtained with any of carbon or metal supplementation. Whereas xylanase yield was elucidated to 5709.2 Ugds -1 by adding Tween 20, SDS repressed its production to 750.29 Ugds -1 . The optimized leaching parameters for effective extraction of xylanase (6312.45 Ugds -1 ) from the fermented solid mixture were found to be citrate buffer (0.1 M, pH 4.0) containing 0.2% Tween 80 as leaching agent, extractant volume 1:8 -1:10 (w/v), soaking time 120 min, leaching pH 4 and leaching temperature 50°C under agitation at 150 rpm. The overall level of 44.61-fold purification of Streptomyces sp. ESRAA-301097 and xylanase recovery 32.52% were achieved with specific activity of 493.48 Umg -1 . The purified enzyme showed a single protein band on SDS-PAGE indicating the monomeric nature of the enzyme with molecular weight ~31.5 kDa. Furthermore, whereas the inhibitors of cysteine protease (1, 10-phenanthroline and Dithiothreitol), metaloprotease (EDTA and EGTA) and thioprotease (iodoacetamide and p-chloromercuribenzoate) had no to minor effects on xylanase activity, the serine protease inhibitor (PMSF) markedly decreased it.

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Thermostable and alkaline-tolerant cellulase-free xylanase produced by thermotolerant Streptomyces sp. Ab106

Cited by 38 publications

References 12 publications

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

A cellulase-free, thermoactive and alkali xylanase production by terrestrial Streptomyces sp. CA24

Optimization of Solid State Fermentation and Leaching Process Parameters for Improvement Xylanase Production by Endophytic Streptomyces sp. ESRAA-301097

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