Thermostable Protease, Amylase and Lipase Enzymes of Thermophilic Bacteria Isolated from Egyptian Hot Springs

Kahled, Eman; Fouad, Mohamed; Badawi, Mona H.; El-Rahim, Wafaa Abd; Shawky, Haitham; Moawad, Hassan

doi:10.21608/ejchem.2022.113659.5190

Cited by 4 publications

(2 citation statements)

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“…The statistical program Minitab 18.1 was used to investigate the effects of independent variables (such as MTX, polymer, FeCl 3 concentration, and pH) on the percent loading efficiency and particle size by using the Placket-Burman experimental design [25]. Two levels of these variables are summarized in Table 1.…”

Section: Design Of the Experimentsmentioning

confidence: 99%

Methotrexate-Polymer Nanocomposites for Targeted Pulmonary Drug Delivery

AL-Sarayrah,

Hussein-Al-Ali,

Haddad

et al. 2024

Indones. J. Chem.

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Nanocomposite formulation is a suitable technology that enables the development of successful dry powder inhalers. The methotrexate (MTX) and polyamide-disulfide (polymer) were used as a model to form MTX-polymer nanocomposites. Different amounts of the independent variable, MTX (0.025 and 0.050 g), polymer (0.05 and 0.01 g), pH (6.7 and 11.3), and across-linker ferric chloride (FeCl3) (0.05 and 0.10 g) were used. The loading efficiency and particle size were dependent variables. The optimized formula can be obtained with the highest loading efficiency and optimum particle size. This formula can be collected by using 0.025 g of drug, 0.079 g of polymer, 0.050 g of FeCl3, and pH = 6.7. The release of MTX from the nanocomposites occurs in two release steps; the first release step starts from the beginning up to 60 min, followed by a continuous release phase within 60 min. The results of the NGI analysis demonstrated that 28.1% of the nominated dose in each puff reached the lower parts of the respiratory system, an indication that the nanocomposites can be used in the delivery of MTX as a respiratory system.

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Section: Design Of the Experimentsmentioning

confidence: 99%

Methotrexate-Polymer Nanocomposites for Targeted Pulmonary Drug Delivery

AL-Sarayrah,

Hussein-Al-Ali,

Haddad

et al. 2024

Indones. J. Chem.

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“…Further, thermophilic bacteria were effective in wastewater treatment especially in the treatment with high temperature (Al-rasheedi et al 2022). In Egypt, 6 thermophilic enzymes were isolated from thermophilic bacteria from thermophilic hot springs include Pharaoh Baths, Ras Sedr, Kharga Oasis and Dakhla Oasis, protease-,lipase-and amylase-producing thermophiles utilized diverse of products that modified several bioindustrial applications (Kahled et al 2022).…”

Section: Staphylococcusmentioning

confidence: 99%

Microbial diversity and abundance in the hot springs on the west coast of Saudi Arabia as a potential source of novel industrial products

AlSaade

Ashy

Al-Fassi

et al. 2022

MBJ

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A knowledge gap exists regarding the potential of thermophilic bacteria in the hot springs in Saudi Arabia as a resource for medically useful compounds. This study aimed to investigate the microbial diversity in the soils of hot springs at three locations on the west coast of Saudi Arabia, including Jizan, Al Lith, and Tabuk. Soil samples were extracted for rDNA 16S, and the results were analysed for microbial diversity and abundance. The results showed the dominance of Acidobacteriota, Actinobacteriota, Desulfobacterota, Firmicutes, Halanaerobiaeota, Nitrospirota, and Thermotogota. Actinobacteriota dominated the microbial population in Al Lith soil, while Deinococcota were dominant in Jizan soil and Chloroflexi in Tabuk soil. Firmicutes and Proteobacteria had high microbial populations in all three soils. Based on principal coordinate analysis, the normalized relative microbial diversity of all soils showed differences as well as similarities in the distribution of taxonomic classifications between soil samples. In conclusion, the microbial diversity in Jizan, Al Lith, and Tabuk included a variety of microorganism species that can potentially be used in biotechnology as a novel source for industrial production of medically useful compounds.

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