Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose

Singer, Mike A.; Lindquist, Susan

doi:10.1016/s0167-7799(98)01251-7

Cited by 446 publications

(336 citation statements)

References 62 publications

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“…Yeasts that cannot eliminate their trehalose suffer when they return to normal temperatures. This has been termed 'the yin and yang of trehalose' (Singer and Lindquist, 1998). This phrase nicely captures the important conclusion arising from these observations, namely that some 'compatible' solutes may be harmful in the absence of a perturbant.…”

Section: Mechanisms Of Stabilizationmentioning

confidence: 79%

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Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses

Yancey

2005

Journal of Experimental Biology

1,574

1,272

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SUMMARY Organic osmolytes are small solutes used by cells of numerous water-stressed organisms and tissues to maintain cell volume. Similar compounds are accumulated by some organisms in anhydrobiotic, thermal and possibly pressure stresses. These solutes are amino acids and derivatives,polyols and sugars, methylamines, methylsulfonium compounds and urea. Except for urea, they are often called `compatible solutes', a term indicating lack of perturbing effects on cellular macromolecules and implying interchangeability. However, these features may not always exist, for three reasons. First, some of these solutes may have unique protective metabolic roles, such as acting as antioxidants (e.g. polyols, taurine, hypotaurine),providing redox balance (e.g. glycerol) and detoxifying sulfide (hypotaurine in animals at hydrothermal vents and seeps). Second, some of these solutes stabilize macromolecules and counteract perturbants in non-interchangeable ways. Methylamines [e.g. trimethylamine N-oxide (TMAO)] can enhance protein folding and ligand binding and counteract perturbations by urea (e.g. in elasmobranchs and mammalian kidney), inorganic ions, and hydrostatic pressure in deep-sea animals. Trehalose and proline in overwintering insects stabilize membranes at subzero temperatures. Trehalose in insects and yeast,and anionic polyols in microorganisms around hydrothermal vents, can protect proteins from denaturation by high temperatures. Third, stabilizing solutes appear to be used in nature only to counteract perturbants of macromolecules,perhaps because stabilization is detrimental in the absence of perturbation. Some of these solutes have applications in biotechnology, agriculture and medicine, including in vitro rescue of the misfolded protein of cystic fibrosis. However, caution is warranted if high levels cause overstabilization of proteins.

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Section: Mechanisms Of Stabilizationmentioning

confidence: 79%

“…However, certain carbohydrate solutes may be used in living organisms to counteract temperature disruption of proteins. For example, heat stress induces accumulation of trehalose in yeast, in which the disaccharide can protect enzymes from thermal denaturation (Singer and Lindquist, 1998).…”

Section: High Temperaturementioning

confidence: 99%

Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses

Yancey

2005

Journal of Experimental Biology

1,574

1,272

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“…60) Exposure of yeast cells to temperatures higher than the optimum for growth (e.g., 42 C) enhances the synthesis of Hsps and the metabolism of trehalose. 61) Then the cells acquire the ability to survive under lethally high temperatures (e.g., 50 C) as well as under other stressful conditions. Among the many Hsps, the molecular chaperone Hsp104 plays an essential role in acquired tolerance by unfolding denatured intracellular proteins in an ATP-dependent manner.…”

Section: Recent Advanced Studies Of the Effects Of High Pressurementioning

confidence: 99%

“…This disaccharide has a role in the piezotolerance of yeast by preventing the formation of protein aggregates and promoting the refolding activity of Hsp104. 61) While yeast mutants defective in the accumulation of trehalose are susceptible to increasing pressure, 64) a mutant lacking neutral trehalase Nth1 that catalyzes the hydrolysis of one trehalose molecule producing two glucose molecules also displays susceptibility to high pressure. 68) Neutral trehalase is required for recovery only after highpressure treatment and is not required during treatment.…”

Section: Recent Advanced Studies Of the Effects Of High Pressurementioning

confidence: 99%

Exploration of the Effects of High Hydrostatic Pressure on Microbial Growth, Physiology and Survival: Perspectives from Piezophysiology

Abe

2007

Bioscience, Biotechnology, and Biochemistry

178

110

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“…Elaborate control mechanisms are strictly coordinated in the regulation of cell wall biogenesis. Stress due to heat shock or ethanol induces the production of trehalose (Attfield, 1987;Hottiger et al, 1987;Neves and Francois, 1992;Hottiger et al, 1994;Singer and Lindquist, 1998) and glycerol (Alonso-Monge et al, 2001), which increase turgor pressure. Defects in the assembly of the cell wall compromise the response to stress and severely threaten cell survival.…”

Section: Introductionmentioning

confidence: 99%

Loss of Function ofKRE5Suppresses Temperature Sensitivity of Mutants Lacking Mitochondrial Anionic Lipids

Zhong

Gvozdenović-Jeremić

Webster

et al. 2005

MBoC

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Disruption of PGS1, which encodes the enzyme that catalyzes the committed step of cardiolipin (CL) synthesis, results in loss of the mitochondrial anionic phospholipids phosphatidylglycerol (PG) and CL. The pgs1⌬ mutant exhibits severe growth defects at 37°C. To understand the essential functions of mitochondrial anionic lipids at elevated temperatures, we isolated suppressors of pgs1⌬ that grew at 37°C. One of the suppressors has a loss of function mutation in KRE5, which is involved in cell wall biogenesis. The cell wall of pgs1⌬ contained markedly reduced ␤-1,3-glucan, which was restored in the suppressor. Stabilization of the cell wall with osmotic support alleviated the cell wall defects of pgs1⌬ and suppressed the temperature sensitivity of all CL-deficient mutants. Evidence is presented suggesting that the previously reported inability of pgs1⌬ to grow in the presence of ethidium bromide was due to defective cell wall integrity, not from "petite lethality." These findings demonstrated that mitochondrial anionic lipids are required for cellular functions that are essential in cell wall biogenesis, the maintenance of cell integrity, and survival at elevated temperature. INTRODUCTIONCardiolipin (CL), a unique anionic phospholipid with dimeric structure, is ubiquitous in eukaryotes and primarily found in the mitochondrial inner membrane . CL plays a key role in mitochondrial bioenergetics (Jiang et al., 2000; Greenberg, 2000, 2002;Schlame et al., 2000;Pfeiffer et al., 2003) and is also involved in mitochondrial biogenesis (Kawasaki et al., 1999;Jiang et al., 2000). Defective remodeling of CL is associated with Barth syndrome, a severe genetic disorder characterized by cardiomyopathy, neutropenia, skeletal myopathy, and respiratory chain defects (Vreken et al., 2000). The phenotype of Barth syndrome is dependent upon multiple factors that are not well understood (Barth et al., 1983(Barth et al., , 1996. Elucidation of the functions of CL will help to clarify the abnormalities associated with this disorder.The biosynthesis of CL is conserved in eukaryotic organisms. It occurs via three enzymatic reactions , including formation of phosphatidylglycerolphosphate (PGP) from CDP-DAG and glycerol-3-P, dephosphorylation of PGP to phosphatidylglycerol (PG), and condensation of CDP-DAG and PG to form CL. Disruption of PGS1, the structural gene encoding PGP synthase, results in the complete loss of both PG and CL (Janitor et al., 1996;Chang et al., 1998a). The crd1⌬ mutant, which lacks CL synthase, has no detectable CL but accumulates PG (Jiang et al., 1997;Chang et al., 1998b;Tuller et al., 1998;Jiang et al., 2000;Pfeiffer et al., 2003;Zhong et al., 2004). The human taffazin gene (TAZ1), which is associated with Barth syndrome, encodes a transacylase that may be involved in the remodeling of CL (Xu et al., 2003). Deletion of the yeast homolog of this gene, TAZ1, leads to decreased CL, aberrant CL acyl species, and accumulation of monolysocardiolipin . Mutants deficient in CL biosynthesis exhibit growth defects at elevat...

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Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose

Cited by 446 publications

References 62 publications

Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses

Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses

Exploration of the Effects of High Hydrostatic Pressure on Microbial Growth, Physiology and Survival: Perspectives from Piezophysiology

Loss of Function ofKRE5Suppresses Temperature Sensitivity of Mutants Lacking Mitochondrial Anionic Lipids

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