Thermotolerant <i>Campylobacter</i> during Broiler Rearing: Risk Factors and Intervention

Robyn, Joris; Rasschaert, Geertrui; Pasmans, Frank; Heyndrickx, Marc

doi:10.1111/1541-4337.12124

Cited by 47 publications

(43 citation statements)

References 318 publications

(544 reference statements)

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“…It has been suggested that a reduction of Campylobacter in the chicken intestine by 1 log 10 CFU can reduce the public health risk by 50% to 90%, and a 2 log 10 CFU reduction can reduce the risk by >90% [8,34,35]. This can be achieved with natural compounds [10].…”

Section: Plos Onementioning

confidence: 99%

(-)-α-Pinene reduces quorum sensing and Campylobacter jejuni colonization in broiler chickens

Šimunović¹,

Şahin²,

Kovač³

et al. 2020

PLoS ONE

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Campylobacter jejuni is one of the most prevalent causes of bacterial gastroenteritis worldwide, and it is largely associated with consumption of contaminated poultry. Current Campylobacter control measures at the poultry production level remain insufficient, and hence there is the need for alternative control strategies. We evaluated the potential of the monoterpene (-)-α-pinene for control of C. jejuni in poultry. The antibacterial and resistance-modulatory activities of (-)-α-pinene were also determined against 57 C. jejuni strains. In addition, the anti-quorum-sensing activity of (-)-α-pinene against C. jejuni NCTC 11168 was determined for three subinhibitory concentrations (125, 62.5, 31.25 mg/L) over three incubation times using an autoinducer-2 bioassay based on Vibrio harveyi BB170 bioluminescence measurements. The effects of a subinhibitory concentration of (-)-α-pinene (250 mg/L) on survival of C. jejuni, and in combination with enrofloxacin on fluoroquinolone resistance development in C. jejuni, were determined in a broiler chicken model, by addition of (-)-α-pinene to the broiler water supply. The reduction of C. jejuni numbers by (-)-α-pinene was further determined in broiler chickens that were colonized with either fluoroquinolone-susceptible or-resistant strains, by direct gavage treatment. We observed weak in vitro antimicrobial activity for (-)-α-pinene alone (MIC >500 mg/L), but strong potentiating effects on antibiotics erythromycin and ciprofloxacin against different Campylobacter strains (>512 fold change). After 24 h of treatment of C. jejuni with (-)-α-pinene, its quorum-sensing signaling was reduced by >80% compared to the untreated control. When given in the drinking water, (-)-α-pinene did not show any significant inhibitory effects on the level of C. jejuni in the colonized chickens, and did not reduce fluoroquinolone resistance development in combination with enrofloxacin. Conversely, when (-)-α-pinene was administered by direct gavage, it significantly reduced the number of fluoroquinolone susceptible C. jejuni in the colonized broiler chickens. These results demonstrate that (-)-α-pinene modulates quorum-sensing in Campylobacter,

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Section: Plos Onementioning

confidence: 99%

(-)-α-Pinene reduces quorum sensing and Campylobacter jejuni colonization in broiler chickens

Šimunović¹,

Şahin²,

Kovač³

et al. 2020

PLoS ONE

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show abstract

“…On the other hand, although results of experiments assessing the efficiency of mitigation strategies aimed at increasing resistance to colonization or reducing the level of the pathogen in caeca are encouraging (Meunier et al, 2016;Robyn, Rasschaert, Pasmans, & Heyndrickx, 2015), further studies to obtain more reproducible results are needed before effective applications of those measures on large scale.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Quantitative risk assessment of Campylobacter in broiler chickens – Assessing interventions to reduce the level of contamination at the end of the rearing period

2017

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“…Many cases are not reported, and as many as 9 million people are estimated to suffer from campylobacteriosis annually in the EU (Havelaar et al 2013). The cost of campylobacteriosis for the member countries of the European Union is between 500 and 5000 million euros per year (EFSA 2011; Robyn et al 2015). Campylobacter jejuni and C. coli are the most frequently reported species in human cases of Campylobacter infection (WHO (World Health Organisation) 2018), causing approximately 90 and 10% of cases, respectively (Gillespie et al 2002;Nielsen et al 2006;EFSA & ECDC 2018, 2018EFSA 2019EFSA , 2019; Table 1).…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the species of Campylobacter colonizing chickens reared for meat

Babacan

Harris

Pinho

et al. 2020

J. Appl. Microbiol.

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Aim To investigate factors influencing Campylobacter spp. colonization of broiler chickens. Methods and Results Campylobacters were isolated from caeca from 319 flocks of two different breeds (199 Cobb and 120 Hubbard), reared as standard (199), Freedom Food/corn fed (57), free‐range (47) or organic (16). The standard category exclusively used Cobb birds slaughtered at 38‐41 days. The Freedom Food/corn‐fed and free‐range Hubbard birds were slaughtered at 49–56 days and the organic flocks at 70 days. Campylobacters were picked at random from direct plates. Both breed of chicken (Hubbard) and age at slaughter were independently associated with increased likelihood of colonization by Campylobacter coli rather than Campylobacter jejuni, but breed could not be separated from other aspects of husbandry with the data available. Conclusions Chickens are frequently colonized by C. jejuni and C. coli and most human infections originate from poultry. In most developed countries approximately 90% of human infections are caused by C. jejuni, but fewer than 10% by C. coli. This might be due to C. coli being less pathogenic than C. jejuni to humans, and/or to chicken meat carrying fewer C. coli than C. jejuni. More investigations are needed into these aspects before it can be concluded that slaughtering older birds from slower‐growing breeds would reduce the risk of human Campylobacter disease. Significance and Impact of the Study Meat from certain breeds of poultry are predominantly colonized by C. coli rather than C. jejuni. More research is needed to understand the impact this may have on the number and severity of human campylobacter infections.

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Thermotolerant Campylobacter during Broiler Rearing: Risk Factors and Intervention

Cited by 47 publications

References 318 publications

(-)-α-Pinene reduces quorum sensing and Campylobacter jejuni colonization in broiler chickens

(-)-α-Pinene reduces quorum sensing and Campylobacter jejuni colonization in broiler chickens

Quantitative risk assessment of Campylobacter in broiler chickens – Assessing interventions to reduce the level of contamination at the end of the rearing period

Factors affecting the species of Campylobacter colonizing chickens reared for meat

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