TheSPL1 tRNA splicing gene ofCandida maltosa andCandida albicans

Plant, Ewan P.; Becher, Dietmar; Poulter, Russell T. M.

doi:10.1002/(sici)1097-0061(199802)14:3<287::aid-yea213>3.0.co;2-u

Yeast

1998

DOI: 10.1002/(sici)1097-0061(199802)14:3<287::aid-yea213>3.0.co;2-u

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TheSPL1 tRNA splicing gene ofCandida maltosa andCandida albicans

Ewan P. Plant¹,

Dietmar Becher²,

Russell T. M. Poulter³

Abstract: The tRNA splicing gene SPL1‐1 has been cloned and sequenced in Saccharomyces cerevisiae (Kolman and Soll, 1993). Sequence adjacent to the LEU2 gene in Candida maltosa showed some homology to the SPL1‐1 gene of S. cerevisiae. This work describes the sequencing of the SPL1 tRNA splicing genes from C. maltosa and C. albicans and the analysis of these genes. Comparison of these sequences and the relationship observed between the LEU2 and SPL1 genes in these yeasts suggests that there may be some synteny amongst va… Show more

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Cited by 3 publications

References 24 publications

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Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

Sychrová

2001

Yeast

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A DNA fragment carrying the LEU2 gene of osmotolerant yeast Zygosaccharomyces rouxii was isolated. The sequenced DNA fragment (2630 bp) contained two ORFs; one of them (1086 bp long, predicting a protein of 362 amino acids) shared a high degree of similarity with LEU2 genes of other yeast species. The cloned DNA fragment fully complemented the leu2 mutations of Saccharomyces cerevisiae and Z. rouxii. The

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Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

Sychrová

2001

Yeast

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Cloning and sequence analysis of the LEU2 homologue gene from Pichia anomala

Rosa

Pérez

Gutiérrez

et al. 2001

Yeast

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The Pichia anomala LEU2 gene (PaLEU2) was isolated by complementation of a leu2 Saccharomyces cerevisiae mutant. The cloned gene also allowed growth of a Escherichia coli leuB mutant in leucine-lacking medium, indicating that it encodes a product able to complement the b-isopropylmalate dehydrogenase deficiency of the mutants. The sequenced DNA fragment contains a complete ORF of 1092 bp, and the deduced polypeptide shares significant homologies with the products of the LEU2 genes from S. cerevisiae (84% identity) and other yeast species. A sequence resembling the GC-rich palindrome motif identified in the 5k region of S. cerevisiae LEU2 gene as the binding site for the transcription activating factor encoded by the LEU3 gene was found at the promoter region. In addition, upstream of the PaLEU2 the 3k-terminal half of a gene of the same orientation, encoding a homologue of the S. cerevisiae NFS1/SPL1 gene that encodes a mitochondrial cysteine desulphurase involved in both tRNA processing and mitochondrial metabolism, was found. The genomic organization of the PaNFS1-PaLEU2 gene pair is similar to that found in several other yeast species, including S. cerevisiae and Candida albicans, except that in some of them the LEU2 gene appears in the reverse orientation. The nucleotide sequence has been submitted to the EMBL database under Accession No. AJ294714.

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Prevalence of small inversions in yeast gene order evolution

Seoighe

Federspiel

Jones

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

138

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Gene order evolution in two eukaryotes was studied by comparing the Saccharomyces cerevisiae genome sequence to extensive new data from whole-genome shotgun and cosmid sequencing of Candida albicans. Gene order is substantially different between these two yeasts, with only 9% of gene pairs that are adjacent in one species being conserved as adjacent in the other. Inversion of small segments of DNA, less than 10 genes long, has been a major cause of rearrangement, which means that even where a pair of genes has been conserved as adjacent, the transcriptional orientations of the two genes relative to one another are often different. We estimate that about 1,100 single-gene inversions have occurred since the divergence between these species. Other genes that are adjacent in one species are in the same neighborhood in the other, but their precise arrangement has been disrupted, probably by multiple successive multigene inversions. We estimate that gene adjacencies have been broken as frequently by local rearrangements as by chromosomal translocations or long-distance transpositions. A bias toward small inversions has been suggested by other studies on animals and plants and may be general among eukaryotes.

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TheSPL1 tRNA splicing gene ofCandida maltosa andCandida albicans

Cited by 3 publications

References 24 publications

Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

Cloning and sequence analysis of the LEU2 homologue gene from Pichia anomala

Prevalence of small inversions in yeast gene order evolution

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