Thiacloprid exposure perturbs the gut microbiota and reduces the survival status in honeybees

Liu, Yong-Jun; Qiao, Nenghu; Diao, Qingyun; Jing, Zhongwang; Vukanti, Raja; Dai, Pingli; Ge, Yuan

doi:10.1016/j.jhazmat.2019.121818

Cited by 77 publications

(54 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…F. perrara is known to have a key role in honeybee immunity in limiting microbial resistance [83], therefore F. perrara disbalance might affect the honeybee gut immunity, leading to microbiota dysbiosis. Finally, the increasing activity of Bi dobacterium is in agreement with previous studies [19][20][21] that tested nitenpyram and thiacloprid exposure on honeybees.…”

Section: Discussionsupporting

confidence: 91%

“…Given that gut microbes are of primary importance in regulating the above mentioned bene cial functions in honeybees [15][16][17][18], recent studies started to focus in understanding relationship between symbiotic microbes and host health [15]. Recent works observed that exposure to neonicotinoids (thiacloprid [19], nitenpyram [20] and imidacloprid [21]) exerted adverse effects on gut microbiota. In addition, the clothianidin (CAS 210880-92-5) was suspected to potentially disrupt the microbiota-immunity axis [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary Contamination With Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity Along the Honeybee (Apis Mellifera) Digestive Tract

Khoury

Gauthier

Bouslama

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundHoneybees (Apis mellifera) are facing a number of interacting stress factors affecting their general health as well as their nervous and immune systems. Recent evidence proposed the clothianidin neonicotinoid as a potential disrupting factor for the microbiota-immunity axis. In this research, we conducted in vivo experiments to measure the impact of three sublethal clothianidin concentrations (0.1, 1 and 10 ppb) on honeybee survival, syrup consumption and the honeybee gut microbiota dysbiosis. Results Clothianidin exposure significantly increased mortality in the three concentrations compared to controls. Interestingly, the lowest clothianidin concentration was associated to the highest food intake and the highest mortality. Clothianidin exposure significantly induced variation in the taxonomic composition of the gut microbiota. The co-abundance network analysis reveals local dysbiosis signatures specific to each gut cohorts (midgut, ileum and rectum) driven by specific taxa.Conclusions Our findings confirm that clothianidin exposure triggers a disruption of the honeybee’s symbiotic defense systems such as resistance to colonization, translating into a surge of potentially pathogenic taxa. Furthermore, the importance of low activity taxa with weak transcriptional activity in maintaining a stable honeybee gut microbiota. Finally, the early detection of gut dysbiosis in honeybee is an early promising alarming signal towards a microbiome approach in hive management the impact for assessing the impact of sublethal xenobiotics exposure.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Dietary Contamination With Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity Along the Honeybee (Apis Mellifera) Digestive Tract

Khoury

Gauthier

Bouslama

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Furthermore, glyphosate reduced the abundance of beneficial bacteria in the honeybee gut in a dose-dependent way [ 109 ]. According to Liu et al [ 110 ], high and very high concentrations of thiacloprid (a neonicotinoid insecticide) led to dysbiosis in the gut microbial community of honeybees. It caused a decrease in total microbial abundance in a dose-dependent manner in three treatment groups of insects.…”

Section: Honeybee Microbiotamentioning

confidence: 99%

Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review

Nowak

Szczuka

Górczyńska

et al. 2021

Cells

View full text Add to dashboard Cite

Numerous honeybee (Apis mellifera) products, such as honey, propolis, and bee venom, are used in traditional medicine to prevent illness and promote healing. Therefore, this insect has a huge impact on humans’ way of life and the environment. While the population of A. mellifera is large, there is concern that widespread commercialization of beekeeping, combined with environmental pollution and the action of bee pathogens, has caused significant problems for the health of honeybee populations. One of the strategies to preserve the welfare of honeybees is to better understand and protect their natural microbiota. This paper provides a unique overview of the latest research on the features and functioning of A. mellifera. Honeybee microbiome analysis focuses on both the function and numerous factors affecting it. In addition, we present the characteristics of lactic acid bacteria (LAB) as an important part of the gut community and their special beneficial activities for honeybee health. The idea of probiotics for honeybees as a promising tool to improve their health is widely discussed. Knowledge of the natural gut microbiota provides an opportunity to create a broad strategy for honeybee vitality, including the development of modern probiotic preparations to use instead of conventional antibiotics, environmentally friendly biocides, and biological control agents.

show abstract

“…The intestinal bacteria might play an important role in honeybees in CCD hives. Therefore, many studies have concentrated on the negative effects of the intestinal bacteria of honeybees, including the effects of abiotic and biotic factors (Cox-Foster et al 2007, Diaz et al 2019, Liu et al 2019). The stability of the intestinal bacteria are important for the health of honeybees.…”

Section: Discussionmentioning

confidence: 99%

“…The intestinal bacteria of a healthy honeybees are characterized by its dynamic stability. However, it can show dysbiosis or disequilibrium when challenged by many factors alone or combination, such as a different diet (Maes et al 2016), pesticide exposure (Liu et al 2019), parasite and pathogen infection (Diaz et al 2019), and behavioral tasks (Jones et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Overwintering honeybees maintained dynamic and stable intestinal bacteria

Liu

Zhu

Liang

et al. 2021

Preprint

View full text Add to dashboard Cite

Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3 − V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.

show abstract

Thiacloprid exposure perturbs the gut microbiota and reduces the survival status in honeybees

Cited by 77 publications

References 48 publications

Dietary Contamination With Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity Along the Honeybee (Apis Mellifera) Digestive Tract

Dietary Contamination With Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity Along the Honeybee (Apis Mellifera) Digestive Tract

Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review

Overwintering honeybees maintained dynamic and stable intestinal bacteria

Contact Info

Product

Resources

About