Thidiazuron Induced Direct Shoot Organogenesis in Stevia rebaudiana and Assessment of Clonal Fidelity of Regenerated Plants by RAPD and ISSR

Singh, Manvendra; Saharan͙, Vinod; Rajpurohit, Deepak; Sen, Yadunandan; Joshi, Arunabh; Sharma, Ajay

doi:10.20546/ijcmas.2017.608.203

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…While increasing TDZ concentration more than 0.5mg/L reduced number of shoots and shoot elongation but increase callus formation from zero to 10mm in diameter. These results seems to be in harmony with those of Singh et al (2017), Keshvari et al (2018).…”

Section: Direct Adventitious Shoot Bud Induction From Leaf Explantssupporting

confidence: 85%

“…Small callus formation less than 5mm diameter was observed on MS medium supplemented with 2.0mg/L IAA and on MS with 1.0 or 2.0mg/L NAA. The results seems to be in harmony with those of Singh et al (2017), Keshvari et al (2018).…”

Section: Adventitious Root Formationsupporting

confidence: 83%

“…In contrast, The findings are in disagreement with Thiyagarajan & Venkatachalam (2012), who found that 1.0mg/L BA + 0.5 IBA combination was given a good growth of shoot bud 4.25 shoots with 5.02cm shoot length. Atalay et al ( 2011), Aman et al (2013), Singh et al (2017) found that the addition of IBA along with BA was efficient using Stevia rebaudiana nodal explants, while IAA was proved the most suitable and efficient auxin for shoot bud multiplication compared to NAA.…”

Section: Direct Adventitious Shoot Bud Induction From Leaf Explantsmentioning

confidence: 99%

“…The growing demand for high-AHMED SAAD ATTAYA quality materials has highlighted the importance of plant tissue culture technology for rapid and mass multiplication. As a result, in vivo and in vitro nodal explants were used to demonstrate high frequency Stevia mass multiplication (Singh et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Direct Shoot Bud Proliferation Protocol from Stevia rebaudiana Leaf Culture for Healthy Biomass Production

Attaya

2021

Egypt. J. Agron.

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Egyptian Journal of Agronomy http://agro.journals.ekb.eg/ Short Note 12 A N EFFICIENT and simple regeneration protocol using leaf explants was described on MS medium in the presence of various plant growth regulators. After four weeks of culture, the highest induction of adventitious shoot buds from leaf culture (90%) was achieved on MS medium with 1.0mg/L BA. The largest number of shoot buds (3.02) per leaf explant with no callus formation was achieved on MS medium with 2.0mg/L BA. The best combination for the induction of numerous shoot buds was found to be TDZ + BA + IBA (0.5, 1.0, and 0.5mg/L, respectively), which presented 10.4 shoots per explant with 3.15cm in length. For in vitro root formation stage, Healthy shoots were collected and cultivated on a half-strength MS with various concentrations of NAA and IAA alone or in combination with 2,4-D or IBA. Furthermore, the highest roots (8.17) per shoot with root formation (100 %) was observed using a half-strength MS medium in the presence of 2.0mg/L IAA. Therefore, healthy in vitro rooted plantlets were acclimatized successfully after four weeks in the greenhouse.

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Section: Direct Adventitious Shoot Bud Induction From Leaf Explantssupporting

confidence: 85%

Section: Adventitious Root Formationsupporting

confidence: 83%