Thidiazuron induced efficientin vitromultiplication andex vitroconservation ofRauvolfia serpentina– a potent antihypertensive drug producing plant

Abstract

An efficient system for in vitro propagation of the endangered medicinal plant Rauvolfia serpentina has been developed. Proliferation of shoots was achieved from nodal segment explants, excised from a field grown plants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) (0.1À2.5 mmol/L) although with low regeneration response and few number of shoots per explant. Greater number of shoots was achieved from nodal explants pretreated with higher concentrations of TDZ (5À100 mmol/L) in liquid MS medium for different time periods (4, 8, 12 and 16 days), followed by their transfer on a growth regulator-free medium. The highest response in terms of percent regeneration (90%), average number of shoots/explant (23.17 § 2.15) and maximum shoot length (5.3 cm § 0.83 cm) were achieved by pretreating the nodal explants with 50 mmol/L TDZ for 8 days. Rooting was achieved in MS medium supplemented with 0.5 mmol/L indole-3-butyric acid with an average of 5.50 § 0.92 roots per microshoot. The rooted plantlets were transferred to soil, where 90% of them grew well and exhibited a normal development. Chlorophyll a and b, carotenoids, net photosynthetic rate and antioxidative enzymes, viz., superoxide dismutase and catalase activities were measured in leaf tissues during ex vitro acclimatization at 0, 7, 14, 21 and 28 days. Most of the activities showed a decreasing trend but subsequently increased after 7 days of acclimatization. These changes suggested the adjustment of the in vitro propagated plantlets to ex vitro conditions.

Preculturing effect of thidiazuron onin vitroshoot multiplication and micropropagation round inCapparis decidua(Forsk.) an important multipurpose plant

“…12 and 16 d and the shoots regenerated were deformed in morphology. Similar results were observed in Hypericum perforatum [15] Cassia angustifolia [24] and Rauvolfia serpentina [4]. To see the potential of the plants produced from the preculturing experiments and its reproducibility, shoots induced from 50 µM TDZ concentration and 8 d duration were again used for three extra rounds of propagation.…”

Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

“…TDZ-supplemented media was previously reported as promoting higher numbers of leaves and nodes on explants in a micropropagation system developed for H. lupulus (Roy et al, 2001). In other species, such as Rauvolfia serpentine, TDZ was also effective in in vitro multiplication (Alatar, 2015).…”

Clonalin vitromultiplication of grey mangrove and assessment of genetic fidelity using single primer amplification reaction (SPAR) methods

“…The highest frequency of root formation (71%), number of roots (2.9 § 0.24) and maximum average root length (3.8 § 0.30) was recorded at 1.0 mmol/L of IBA after 4 weeks of culturing. The efficacy of IBA in root induction has been reported in Rauvolfia serpentina, [5] Mentha arvensis, [16] Sansevieria cylindrica [25] and in Cyamopsis tetragonoloba [26]. Plantlets having 4À5 fully expanded leaves and well-developed roots were successfully hardened off in the growth room in pots containing sterile soilrite mixture and covered with transparent polybags to maintain high humidity.…”

“…The application of biotechnological approaches to plants would also benefit from the development of tissue culture systems for in vitro development and regeneration of rare and endangered species. [4,5] Tissue culture techniques could be a cost-effective means of bulk production of elite planting material throughout the year without any seasonal constraints. It may also play an essential role in clonal mass multiplication and ex situ preservation of germplasm that is rare, endangered or on the edge of extinction.…”