Thidiazuron-induced morphogenesis in tamarind seedlings

Mehta, Urmil J.; Sahasrabudhe, N.A.; Hazra, Sulekha

doi:10.1079/ivp2004611

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…Meristematic regions are known to be highly susceptible to the action of TDZ, which favors the direct induction of adventitious buds (Mehta et al 2005). Similarly, PPCs have demonstrated a highly retained meristematic capacity when located close to the shoot apical meristem.…”

Section: Unicellular Somatic Embryogenesis and Secondary Organogenesismentioning

confidence: 99%

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Almeida

Almeida²,

Graner

et al. 2012

Plant Cell Rep

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Section: Unicellular Somatic Embryogenesis and Secondary Organogenesismentioning

confidence: 99%

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Almeida

Almeida²,

Graner

et al. 2012

Plant Cell Rep

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“…The dome-shaped swelling appearing at the meristem after exposure to TDZ was possibly a result of development of meristematic masses on proliferation of meristematic cells. Proliferation of meristematic cells in response to TDZ exposure was reported in peanut (Joshi et al 2003) and cotyledonary node meristem of tamarind (Mehta et al 2005). Withdrawal of TDZ reduced the negative influence resulting in formation of shoot primordia from the dome-shaped meristem.…”

Section: Resultsmentioning

confidence: 74%

“…In tamarind, the meristem was in the cotyledonary node of the seedling (juvenile tissue), whereas in Pongamia, the meristem was in the axil of the mature-tree-derived nodal segment. In tamarind (Mehta et al 2005), histological studies revealed multiple layers of meristematic cells on and around the meristem as a result of proliferation of the meristematic cells. Caulogenic buds originated from these cells upon withdrawal of TDZ.…”

Section: Resultsmentioning

confidence: 99%

“…In Pongamia, shoot primordia appeared from the TDZ-induced swelling after two passages of 2 wk each in PGR-free medium. Keeping in mind the influence of TDZ on the proliferation of meristematic cells in tamarind (Mehta et al 2004(Mehta et al , 2005, it is presumed that the swelling of the axillary bud in Pongamia was a result of proliferation of the meristematic cells inside the dome cover. Removal of the dome cover exposed a pair of swollen structures (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Micropropagation of mature Pongamia pinnata Pierre

Sujatha

Hazra

2007

In Vitro Cell.Dev.Biol.-Plant

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Murashige and Skoog's (MS) basal medium with benzylaminopurine (BA), kinetin (KN), zeatin (Z), and thidiazuron (TDZ) were tested for induction of multiple shoots from mature-tree-derived axillary meristems of Pongamia pinnata. Sprouting of buds was 64% on medium devoid of plant growth regulators (PGR). Incorporation of BA, KN, or Z was ineffective in enhancing sprouting frequency or induction of multiple shoots. Sprouting was completely suppressed in the presence of TDZ. Caulogenic buds appeared in nodal meristems of these explants after withdrawal of TDZ. The number of shoot buds was more on explants precultured in higher concentrations. At higher concentrations of this PGR, a swelling developed at the axil. Multiple shoot primordia appeared and differentiated from this swelling after culturing these explants on MS medium for six passages of 2 wk each. Shoots were harvested and cultured on 0.45 μM TDZ for further proliferation. Primary explants after harvesting of shoots were identified as 'stump'. Reculturing of stumps on 0.45 μM TDZ produced more shoots. This step was followed for six cycles to obtain additional shoots in each cycle. Shoots maintained on 0.45 μM TDZ elongated and rooted (70%) on growth regulator-free medium. Rooted shoots (65%) survived transfer to a sand/soil mixture. This report describes the protocol for micropropagation of P. pinnata using mature-tree-derived nodal meristems. Recycling of mature stock to produce a stream of useable shoots for subculturing and eventual stabilization is of great value and can possibly be generalized as an isolation protocol especially for woody species. Repeated proliferation of caulogenic buds from the same origin may also find application in rescue of endangered germplasm.

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“…Effect of TDZ on multiplication/ multiple shoot induction TDZ, is a phenylurea derivative known to exert cytokinin like action. It was reported that exposure of explants to an optimum TDZ supplemented medium followed by the withdrawal of growth regulators effectively triggers shoot multiplication in many plant species such as Psoralea corylifolia [43], Tamarindus indica [44], Saintpaulia ionantha [45], Medicago truncatula Gaertn. cv.…”

Section: Effect Of Auxines and Cytokininsmentioning

confidence: 99%

Clonal propagation of female plants of Garcinia indica Choiss: a tree species of high medicinal value

2014

J App Biol Biotech

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Fruits of Garcinia indica, a medicinally important tree species from Western Ghats of India is a rich source of medicinally important (-) Hydroxycitric acid that shows anticholesterol and antiobesity activities. Since this species is polygamodioecious, sex of the plant can be identified only after flowering that takes approximately 7-8 yrs. From commercial and horticultural point of view, plantation of female trees is much preferred. In this study, an efficient protocol for propagation of female plants was developed using in vitro techniques. For establishment of sterile cultures, fungal contamination and browning of explants were the major hurdles. Season of plant material collection and culture vessels used for in vitro sprouting were also observed significant factors in establishment of sterile cultures. The use of WPM basal fortified with 2% sucrose and 8.87 µM BAP resulted maximum sterile and healthy buds. TDZ in the range 0.22 -4.54 µM along with BAP induced multiple shoots. Highest 63% rooting was obtained on WPM medium with 4.90 µM IBA whereas, maximum 60% plantlets survived in green house after their transfer to soil.

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Thidiazuron-induced morphogenesis in tamarind seedlings

Cited by 7 publications

References 15 publications

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study

Micropropagation of mature Pongamia pinnata Pierre

Clonal propagation of female plants of Garcinia indica Choiss: a tree species of high medicinal value

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