Thin Cell Layers: Power-Tool for Organogenesis of Floricultural Crops

Abstract

Thin cell layers or TCLs have been a fundamental corner-stone of tissue culture of all plant species, including floricultural and ornamental plants (FOPs). In this review, the current status of the use of TCL technology to specific FOPs will be outlined while certain successes and difficulties will be focused. The ability to control developmental and morphogenetic processes in vitro through the use of TCLs, especially where conventional methods have not always produced ideal results, is the key in taking lab-based theory to business-based applications. TCL technology, which focuses more on the size rather than the origin of the explant, is a gateway for FOP regeneration and transformation studies. Some general principles and guidelines for quality verification will be provided.

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

“…The TCL culture may induce specific morphogenic routes, such as controlled somatic embryo production (Nhut et al 2003). The technique has proven efficient at propagating many plant species (Silva et al 2007;Silva 2010) species, has been used with peach palm (Bactris gasipaes) by Steinmacher et al (2007c) and oil palm (Elaeis guineensis), with leaf explants (Scherwinski-Pereira et al 2010) and immature inflorescences (Guedes et al 2011). …”