Thin-layer chromatographic determination of diazepam, phenobarbitone, and saccharin in toddy samples

Electroanalytical determination of diazepam in tablet and human serum samples using a multiwalled carbon nanotube embedded molecularly imprinted polymer-modified carbon paste electrode

“…It is also an abused drug in which sudden withdrawal, particularly from high doses, carries the risk of epileptic seizures. 2 A variety of analytical methods have been developed for the determination of this drug in both pharmaceutical and biological samples, such as spectrophotometry, [3][4][5][6] different chromatographic methods (HPLC, LC/MS, GC, GC/MS, and TLC), [7][8][9][10][11] dispersive liquid-liquid microextraction, 12 capillary electrophoresis, 2 radioimmunoassay 13 and electrochemical methods. [14][15][16][17][18][19] Among these methods, electrochemical techniques are a powerful and versatile analytical technique that offers high sensitivity, accuracy, and precision as well as a large linear dynamic range, with relatively low-cost instrumentation than other methods.…”

Detection Progress of Selected Drugs in TLC

“…Sensitivity for phenytoin, alprazolam, diazepam, lorazepam, nitrazepam, and oxazepam is equal to 5  μ g, 20  μ g, 30  μ g, 5  μ g, 30  μ g, and 5  μ g, respectively [54]. A single and sensitive chromogenic chlorination with the use of o-tolidine was used for detection of diazepam, phenobarbitone, and saccharin [55]. Diazepam, phenobarbitone, and saccharin solution were spotted on silica gel G plates and developed by the use of one from three mobile phases, namely, n-hexane-acetone-methanol (8 : 3 : 0.5, v/v/v), n-hexane-acetone-butanol (12 : 8 : 0.5, v/v/v), and chloroform-acetic acid (9 : 1, v/v).…”

“…When a faint blue color appeared the plates were next sprayed with 1% phosphomolybdic acid for stabilization. The limits of detection for diazepam, phenobarbitone, and saccharin with o-tolidine reagent were 0.5 μ g per spot, 0.3 μ g per spot, and 0.1 μ g per spot, respectively [ 55 ]. Cyclodol and diprazin were separated on silica gel by the use of one from six mobile phases: toluene-acetone-ethanol-25% NH 4 OH (45 : 45 : 7.5 : 2.5, v/v/v/v), hexane-ethyl acetate (15 : 5, v/v), chloroform-heptene-25% NH 4 OH (16 : 3 : 3, v/v/v), ethyl acetate-hexane (1 : 1, v/v), acetonitrile-methanol (1 : 1, v/v) or heptene-chloroform-ethanol-25% NH 4 OH (5 : 10 : 3 : 1, v/v/v/v).…”

Forensic Science

“…Quantitation of atenolol, metoprolol, and propranolol in postmortem human fluid and tissue specimens was accomplished by LC/ APCI-MS (523). The thin-layer chromatographic determination of diazepam, phenobarbitone, and saccharin in toddy samples has been reported (524). A simple and modern method for the simultaneous analysis of nitrite and nitrate in whole blood was devised by ion chromatography with an autosuppressor and a conductivity detector (525).…”