Detection Progress of Selected Drugs in TLC

Pyka, Alina

doi:10.1155/2014/732078

Cited by 23 publications

(11 citation statements)

References 96 publications

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“…Noda dengan Rf 0,65 setelah disemprot dengan pereaksi ferri klorida memberikan warna coklat. Hasil ini menunjukkan noda tersebut merupakan senyawa fenolik (asam fenolat) yang diduga merupakan asam vanilat [15]. Untuk noda dengan Rf 0,78 menunjukkan perubahan warna menjadi warna coklat setelah disemprot dengan pereaksi Liebermann-Burchard dan setelah dipanaskan.…”

Section: Fraksiunclassified

Aktivitas Antibakteri Fraksi Serbuk Kayu Eboni (Diospyros celebica Bakh.) terhadap Escherichia coli dan Staphylococcus aureus

2020

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Eboni (Diospyros celebica Bakh.) merupakan salah satu pohon khas daerah Sulawesi. Serbuk kayu sebagai hasil sampingan dari pengolahan kayu ini belum dimanfaatkan secara maksimal. Hasil penelitian pendahuluan menyatakan bahwa ekstrak etanol serbuk kayu eboni memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk melakukan pemisahan senyawa antibakteri yang terdapat di dalamnya dengan metode fraksinasi cair-cair menggunakan pelarut n-heksan, etil asetat, n-butanol, dan air, serta pengujian aktivitas antibakteri dari fraksi-fraksi tersebut dengan metode disk diffusion dan KLT bioautografi. Identifikasi golongan senyawa dari fraksi aktif menggunakan pereaksi kromogenik. Hasil penelitian menunjukkan bahwa fraksi n-heksan memiliki diameter hambat tertinggi terhadap Escherichia coli yakni sebesar 12,53 mm dengan golongan senyawa yang memiliki aktivitas penghambatan adalah steroid dan fraksi etil asetat memiliki diameter hambatan tertinggi terhadap Staphylococcus aureus yakni sebesar 14,77 mm dengan golongan senyawa yang memberikan zona hambat yaitu terpenoid, fenolik, steroid.

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Section: Fraksiunclassified

Aktivitas Antibakteri Fraksi Serbuk Kayu Eboni (Diospyros celebica Bakh.) terhadap Escherichia coli dan Staphylococcus aureus

2020

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“…TLC method has been successfully used for separation of many organic compounds such as vitamins, amino acids, peptides, alkaloids, flavonoids, carbohydrates, drugs etc. (Dolowy and Pyka 2014 ; Koch et al 2013 ; Malinowska et al 2013 ; Mohammad et al 2012 ; Pyka 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid Screening of Antimicrobial Synthetic Peptides

Jaśkiewicz

Orłowska

Olizarowicz

et al. 2015

Int J Pept Res Ther

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Increasing resistance to conventional antibiotics among microorganisms is one of the leading problems of medicine nowadays. Antimicrobial peptides are compounds exhibiting both antibacterial and antifungal activities. However, it is difficult to predict whether a designed new compound would exhibit any biological activity. Moreover, purification of the peptides is one of the most time-consuming and expensive steps of the synthesis that sometimes leads to unnecessary loss of solvents and reagents. In our study we have developed a thin-layer chromatography (TLC) direct bioautography technique for rapid determination of antimicrobial activity of peptides without the necessity of high-performance liquid chromatography purification. In this assay, crude peptides were applied and separated on a TLC plate. Then, pre-prepared plates were dipped into microbial suspension and incubated under optimum conditions for bacteria and fungi as well. The activity of the tested compounds was visualized by spraying the TLC plates with a cell viability reagent, resazurin (7-hydroxy-3H-phenoxazin-3-one 10-oxide). Effectiveness of this assay was compared with minimal inhibitory concentration results obtained by broth microdilution assay. Interestingly, so far such a screening method has not been applied for this group of compounds.

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“…The detection of various drugs by TLC was described in our earlier articles (2,3). An extensive literature review reveals that till today the TLC detection of meloxicam on chromatographic plates is most often carried out under UV light without any visualization agent (4)(5)(6)(7)(8)(9)(10)(11).…”

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confidence: 99%

A cost-effective and sensitive TLC-densitometric identification of meloxicam

2020

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The influence of different chromatographic conditions and the process of spot visualization on determining the limit of detection as well as quantification (LOD and LOQ) of meloxicam by TLC-densitometric technique was estimated. Of all chromatographic conditions tested, the lowest limiting values, thus the best sensitivity, in the NP-TLC system was achieved on silica gel 60F254 and neutral aluminum oxide plates developed with the mobile phase consisting of ethyl acetate/toluene/n-butylamine (2:2:1, V/V/V). In the case of the RP-TLC method, a mixture of methanol/water (8:2, V/V) enabled densitometric detection of meloxicam at the lowest concentration level on RP-8F254 and RP-18F254 plates. Additionally, the smallest LOD value of meloxicam ensured crystalline violet and gentian violet as visualization agents on silica gel 60F254 and neutral aluminum oxide 150F254 plates, resp. Comparison of the densitometrically obtained spectra of meloxicam drug and its standard after the use of appropriate visualization agents could be a good and cheap alternative tool for the identification of meloxicam as an active pharmaceutical ingredient.

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Detection Progress of Selected Drugs in TLC

Cited by 23 publications

References 96 publications

Aktivitas Antibakteri Fraksi Serbuk Kayu Eboni (Diospyros celebica Bakh.) terhadap Escherichia coli dan Staphylococcus aureus

Aktivitas Antibakteri Fraksi Serbuk Kayu Eboni (Diospyros celebica Bakh.) terhadap Escherichia coli dan Staphylococcus aureus

Rapid Screening of Antimicrobial Synthetic Peptides

A cost-effective and sensitive TLC-densitometric identification of meloxicam

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