Thin-layer Chromatographic Identification of Chinese Propolis Using Chemometric Fingerprinting

Tang, Tie-xin; Guo, Weiyan; Xu, Ye; Zhang, Siming; Xu, Xinjun; Wang, Dongmei; Zhao, Zhimin; Zhu, Long‐Ping; Yang, Dongzi

doi:10.1002/pca.2502

Cited by 33 publications

(13 citation statements)

References 19 publications

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“…A combination of hydrogen peroxide and ammonia vapours is used as the visualization reagent in this TLC method, which has been optimized to characterize the chemical profile of Chinese propolis. The results demonstrate the success of a proposed TLC identification strategy of Chinese propolis using chemometric fingerprint, which provides accurate sample classification [64,65]. A specific example of this technique is illustrated in Figure 2.…”

Section: Fingerprintmentioning

confidence: 69%

A Great Concern Regarding the Authenticity Identification and Quality Control of Chinese Propolis and Brazilian Green Propolis

Sun¹,

He²,

Liu³

et al. 2019

JFNR

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Propolis (bee glue) is a natural resin mixture produced by honey bees and it is composed of substances collected from parts of plants, buds and exudates. The composition of propolis varies primary on the geographical locations of the ingredients obtained by the bees. A series of biological properties of propolis including antimicrobial, immunostimulatory, anti-inflammatory, anti-tumor and anti-oxidation activities have been reported in relation to their chemical composition, plant origin and geographical area. This review collates the scientific literature regarding Chinese propolis and Brazilian green propolis, specifically focusing on their different constituents and biological activities. While there are known differences in composition of these two kinds of propolis, they share considerable similarity in their overall chemical composition. Importantly, techniques such as principal component analysis (PCA) and fingerprint are viable options for authenticity identification and quality control of propolis. Many of the molecular mechanisms driving various biological activities of propolis have been determined. We have also found that the biological activity of propolis mainly depends on its constituents, such as flavonoids or phenolic compounds. Each activity is regulated by a variety of biological pathways. The compounds in both Chinese propolis and Brazilian green propolis were found to participate in different biological pathways through distinct molecular mechanisms due to their diverse chemical compositions and unique structures. With the growing market interest of Brazilian green propolis in China, an evaluation between the chemical diversity and biological activity of Chinese propolis and Brazilian green propolis are in need of a review.

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Section: Fingerprintmentioning

confidence: 69%

A Great Concern Regarding the Authenticity Identification and Quality Control of Chinese Propolis and Brazilian Green Propolis

Sun¹,

He²,

Liu³

et al. 2019

JFNR

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“…Visualization and quantification of the separated molecular species are carried out by using short-and long-wavelength UV-light but also, in some specific application, by spraying on the plate different reagents. Due to its complexity and time-consuming characteristics along with its poor resolution, TLC is now rarely applied even if it may be of some utility in specific applications (Milojković Opsenica et al, 2016;Tang et al, 2014).…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

Recent advances in analytical approaches for the standardization and quality of polyphenols of propolis

Galeotti¹,

Capitani²,

Fachini³

et al. 2019

J. Med. Plants Res.

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Analytical approaches utilized for the characterization of polyphenols from propolis useful for the determination of its quality is investigated in this study. A qualitative and quantitative evaluation of propolis bioactive molecules is of interest in medicine and nutraceuticals. Recent powerful analytical techniques are of great utility to separate and quantify polyphenols in extracts and finished products due to their capacity to produce typical fingerprints and a reliable identification of many components. According to this, an HPLC-UV-MS procedure was validated and applied for the characterization and quantification of bioactive substances in propolis and for an accurate assessment of their content in extract samples. By using this analytical approach, we obtained specific compositions related to brown propolis acquired from different geographic areas (and preparations and treatment). This is more important by considering the scientific opinion of European Food Safety Authority (EFSA) which provided a negative response related to health claims of propolis and its polyphenols. These results prove that HPLC-MS is an attractive tool for the standardization and quality control of propolis and may be realistically applied to screen raw material and to evaluate finished commercial preparations and nutraceutical benefits.

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“…Metabolomic profile data have been utilized to distinguish diverse propolis from different geographic origins. Numerous metabolomic research works on the chemical compositions of propolis by various techniques, including thin layer chromatography [23], high-performance liquid chromatography (HPLC) [24], ultra-high-performance liquid chromatography coupled with mass spectrometry [25], and gas chromatography coupled with mass spectrometry [26], have been reported. The NMR technology as a significant spectroscopic method with fast, robust, reproducible, and non-destructive features can provide the unbiased structural information of organic samples.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Reveals Discrimination of Chinese Propolis from Different Climatic Regions

Wang

Liu

Wang

et al. 2020

Foods

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The chemical profiles of propolis vary greatly due to the botanic sources and geographic origins, which limit its standardization for modern usages. Here, we proposed a reliable 1H NMR-based metabolomic approach, to discriminate the function and quality of Chinese propolis. A total 63 Chinese propolis samples from different temperate regions were collected and extracted for NMR analysis. Twenty-one compositions in ethanol extracts were assigned based on characteristic chemical shifts and previous literature reports. Significant geographic indicators were identified after the PCA and orthogonal partial least squares discriminant analysis (OPLS-DA) analysis of the obtained 1H NMR data. It was found that the composition discriminations arose from long-term acclimation of the different climates of botanic origin and caused the differences in the biological activities. This study provides us a reasonable instruction for the quality control of Chinese propolis.

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Thin-layer Chromatographic Identification of Chinese Propolis Using Chemometric Fingerprinting

Abstract: The study has shown that the TLC identification method using chemometric fingerprinting is a rapid, low-cost method for the discrimination of Chinese propolis from poplar tree gum and may be used for the quality control of Chinese propolis.

Cited by 33 publications

References 19 publications

A Great Concern Regarding the Authenticity Identification and Quality Control of Chinese Propolis and Brazilian Green Propolis

A Great Concern Regarding the Authenticity Identification and Quality Control of Chinese Propolis and Brazilian Green Propolis

Recent advances in analytical approaches for the standardization and quality of polyphenols of propolis

Metabolomics Reveals Discrimination of Chinese Propolis from Different Climatic Regions

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