Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds

Cheng, Sy-Chyi; Bhat, Suhail Muzaffar; Lee, Chi‐Wei; Shiea, Jentaie

doi:10.1016/j.ijms.2018.09.024

Cited by 11 publications

(6 citation statements)

References 45 publications

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“…Usually, the identification is laborious, extensive sample preparation is needed and isolation techniques prior to analyses are required to obtain accurate identifications. Liquid chromatography mass spectrometry (LC‐MS) is the gold standard for analyses of plant extracts, although due to the need of sample preparation and long analysis times, there is room for the discovery of rapid and high‐throughput methods 11,12 …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Thin layer chromatography (TLC) is a fast, robust, inexpensive and powerful technique that can be used to separate and analyse complex mixtures without extensive sample preparation 12,16 . It is widely used to separate plants components, in extracts, oils, tinctures, and others 12,17 . Coupling TLC with DESI‐MS creates a fast and powerful technique, that combines the robust separation of TLC with a mass spectrometer as the detector to identify compounds with good selectivity and sensitivity 18,19 .…”

Section: Introductionmentioning

confidence: 99%

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Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSⁿ

Conceição

Reis

Cerqueira

et al. 2020

Phytochemical Analysis

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Introduction: Lauraceae alkaloids are a structurally diverse class of plant specialised secondary metabolites that play an important role in modern pharmacotherapy, being useful as well as model compounds for the development of synthetic analogues. However, alkaloids characterisation is challenging due to low concentrations, the complexity of plant extracts, and long processes for accurate structural determinations. Objective: The use of high-performance thin layer chromatography coupled with desorption electrospray ionisation multistage mass spectrometry (HPTLC DESI-MS n) as a fast tool to identify alkaloids present in Ocotea spixiana extract and evaluate the extract's acaricide activity. Methods: Ocotea spixiana twigs were extracted by conventional liquid-liquid partitioning. HPTLC analysis of the ethyl acetate extract was performed to separate isobaric alkaloids prior to DESI-MS n analysis, performed from MS 3 up to MS 7. The extract's acaricide activity against Rhipicephalus microplus was evaluated by in vitro (larval immersion test) and in silico tests. Results: HPTLC-DESI-MS n analysis was performed to identify a total of 13 aporphine and four benzylisoquinoline-type alkaloids reported for the first time in O. spixiana. In vitro evaluation of the extract and the alkaloid boldine showed significant activity against R. microplus larvae. It was established in silico that boldine had important intermolecular interactions with R. microplus acetylcholinesterase enzyme. Conclusion: The present study demonstrated that HPTLC-DESI-MS n is a useful analytical tool to identify isoquinoline alkaloids in plant extracts. The acaricide activity of the O. spixiana ethyl acetate extract can be correlated to the presence of alkaloids. K E Y W O R D S acaricide activity, alkaloids, HPTLC-DESI-MS n , Ocotea spixiana 1 | INTRODUCTION Ocotea is an expressive genus of the Lauraceae family, occurring mainly in tropical and subtropical areas. 1 Species of this genus are known for producing a variety of secondary metabolites, with neolignans, aporphine and benzylisoquinoline-type alkaloids as the most representative classes. 2,3 A wide range of biological activities from Ocotea species has been described in the literature, including

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSⁿ

Conceição

Reis

Cerqueira

et al. 2020

Phytochemical Analysis

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“…The agar tube dilution method was used for antifungal activity of the extract as reported by Choudary et al [55].…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

Microbial and Plant Assisted Synthesis of Cobalt Oxide Nanoparticles and Their Antimicrobial Activities

Mubraiz

Bano

Mahmood³

et al. 2021

Agronomy

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The development of sustainable, ecofriendly, and cost-effective methods for the synthesis of nanomaterials is an important aspect of nanotechnology these days. The present study was aimed at synthesizing cobalt oxide (Co3O4) nanoparticles by using plant extracts of Aerva javanica, bacterial isolates from rhizospheric soil of Potentilla atrosanguinea, Swertia petiolata, Senecio chrysanthemoides, and from fungus Fusarium oxysporum. X-ray diffraction spectroscopy (XRD) and scanning electron microscopy (SEM) techniques were used in the characterization of the synthesized nanoparticles. The bacterial strain, Bacillus subtilis, isolated from rhizosphere of Potentilla atrosanguinea (N1C1), Fusarium oxysporum, methanolic and aqueous extracts of Aerva javanica reduced the cobalt salts to cobalt oxide nanoparticles. The nanoparticles, synthesized from bacterial isolate N1C1 (Bacillus subtilis) and from Fusarium oxysporum had average particle size of 31.2 nm and 33.4 nm, respectively, whereas, the particle size of Aerva javanica was higher (39.2 nm) and all the nanoparticles were poly shaped. The nanoparticles synthesized from methanolic extract of Aerva javanica, bacterial strain (N1C1) and fungi Fusarium oxysporum showed better performance against Bacillus subtilis and P. aeruginosa, the bactericidal activity was higher against Gram-positive bacterial strains. Methanolic extracts of leaf and flower have shown a wide range of phytochemicals and higher antibacterial activity, and among all strains, Pseudomonas aeruginosa and Bacillus subtilis susceptibility was greater to extracts.

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“…Subsequently, the column was eluted with 80 : 1, 50 : 1, 20 : 1, 5 : 1, 1 : 2, 100% methanol and water [20]. Sub-fractions were analysed on thin layer chromatography plates as well as in HPLC [21,22].…”

Section: Quantitative Phytochemical Screeningmentioning

confidence: 99%

Phytochemical, Antioxidant and Mitochondrial Permeability Transition Studies on Fruit-Skin Ethanol Extract ofAnnona muricata

Iyanda-Joel

Ajetunmobi

Chinedu

et al. 2019

Journal of Toxicology

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Uncontrolled cell proliferation hallmarks cancer and most cancer cells have developed multiple resistance to the drugs employed for their treatment. The study examined the phytochemical and antioxidant properties of the fruit-skin ethanol extract of Annona muricata Linn. (ESA) and its effect on rat liver mitochondrial membrane permeability transition (MMPT). Qualitative phytochemical study and antioxidant assays were carried out following established protocols while the opening of the MMPT pore in the presence of varying concentrations of the extract was assayed spectrophotometrically under succinate-energized conditions. Calcium chloride (CaCl2) and spermine were used to trigger and inhibit pore opening respectively. Cytochrome c release was assayed for using ELISA kit. Terpenoids, steroids, phenols among other phytochemicals were found present in ESA and the extract showed very low antioxidant properties at the tested concentrations based on the diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity assay. Lipid peroxidation was induced in a concentration-dependent manner on both the cytosolic and mitochondrial hepatocyte fractions in vitro. In the absence of CaCl2 0.84 mg/mL concentration of ESA induced MMPT pore opening by 129% while the extracts showed no inhibitory activity in its presence. The induction fold corresponded with the concentrations of cytochrome c released. The fruit-skin ethanol extract of Annona muricata at certain concentrations may possibly contain bioactive compounds that induce apoptosis.

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Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds

Cited by 11 publications

References 45 publications

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSⁿ

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSⁿ

Microbial and Plant Assisted Synthesis of Cobalt Oxide Nanoparticles and Their Antimicrobial Activities

Phytochemical, Antioxidant and Mitochondrial Permeability Transition Studies on Fruit-Skin Ethanol Extract ofAnnona muricata

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Thin layer chromatography combined with electrospray ionization mass spectrometry for characterizing herbal compounds

Cited by 11 publications

References 45 publications

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSn

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSn

Microbial and Plant Assisted Synthesis of Cobalt Oxide Nanoparticles and Their Antimicrobial Activities

Phytochemical, Antioxidant and Mitochondrial Permeability Transition Studies on Fruit-Skin Ethanol Extract ofAnnona muricata

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Product

Resources

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Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSⁿ

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSⁿ