Thin layer chromatography/mass spectrometry without substance elution and gas chromatography in the study of organophosphorus pesticides metabolism

Fogy, I.; Allmaier, Günter; Schmid, E.

doi:10.1016/0020-7381(83)87092-2

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Coanalysis Of Sample and Adsorbent1

Introduction1

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1987

2003

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Cited by 13 publications

(3 citation statements)

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“…Fogy et al (35) used TLC/MS to study degradation products of organophosphorus pesticides. Polyamide 6 was used as the TLC layer material for separation.…”

Section: Coanalysis Of Sample and Adsorbentmentioning

confidence: 99%

Thin-Layer Chromatography Coupled with Mass Spectrometry

Busch¹

2003

Handbook of Thin-Layer Chromatography

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“…Fogy et al (35) used TLC/MS to study degradation products of organophosphorus pesticides. Polyamide 6 was used as the TLC layer material for separation.…”

Section: Coanalysis Of Sample and Adsorbentmentioning

confidence: 99%

Thin-Layer Chromatography Coupled with Mass Spectrometry

Busch¹

2003

Handbook of Thin-Layer Chromatography

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“…A second method involves analysis of a small piece of TLC cut from the plate. MS is performed in the presence of the stationary phase [5][6][7]. These two methods both require an independent visualization method to locate the analyte spots and do not allow 2-D imaging.…”

Section: Introductionmentioning

confidence: 99%

Coupling protocol for thin layer chromatography/matrix-assisted laser desorption ionization

1997

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SummaryThis paper reports the investigation and optimization of the thin layer chromatography/matrix-assisted laser desorption/ionization (TLC-MALDI) coupling protocol. The fundamental coupling parameters which influence sensitivity and lateral analyte spreading are extraction solvent selection, extraction time, and pressure. Selection of the solvent is dependent upon its extraction efficiency, which has been correlated with extraction solvent Rf value. Maximum extraction efficiency occurs after two minutes for the analyte/solvent system studied. High extraction efficiency solvents cause significant lateral spreading of analyte; up to a three-fold increase in initial analyte spot size. Analyte recovery was found to be limited by the silica gel inter-particle porosity and the solvent extraction efficiency. For maintaining chromatographic resolution and maximizing signal intensity, extraction solvents with Rf values between 0.4 to 0.6 are optimal. The upper analyte recovery limit, using extraction solvents within this Rf range, is estimated at 22 %.

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“…Organophosphorus pesticides are of interest due to their wide use in agriculture and, in some cases, high mammalian toxicity. Numerous analytical techniques have been devised for these compounds (6)(7)(8)(9)(10)(11)(12)(13)(14)(15); but these techniques are often compound or class specific, often require extensive sample preparation, or the analytical response varies considerably. This last point is especially true for thermospray HPLC-MS of organophosphorus compounds (7,16,17).…”

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confidence: 99%