Thio-glucose bound gold nanoparticles enhance radio-cytotoxic targeting of ovarian cancer

Geng, Feng; Song, Kun; Jin, Xing; Yuan, Cunzhong; Shi, Yan; Yang, Qifeng; Chen, Jie; Kong, Beihua

doi:10.1088/0957-4484/22/28/285101

Cited by 155 publications

(112 citation statements)

References 23 publications

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“…Proliferation assays were performed as previously described (Geng et al, 2011). Cells were seeded in a 96-well plate with 200 ml medium, 3-(4,5)-dimethylthiahiazo (-2-y1)-2,5-diphenytetrazoliumromide (MTT, Sigma, St. Louis, MO) was added at a concentration of 0.5 mg/ml, and the cells were incubated for 4 h at 37 C. The medium was removed, and dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO) was added to each well.…”

Section: Measurement Of Cell Proliferationmentioning

confidence: 99%

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

Lu¹,

Liu²,

Yuan³

et al. 2013

Pharmaceutical Biology

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(2013) The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts, Pharmaceutical Biology, 51:6, 712-721, DOI: 10.3109/13880209.2013 Context: Qindan capsule (QC), a compound used in traditional Chinese medicine, has been used as an anti-hypertensive agent in clinical settings for years. Our previous studies have shown that QC can improve the morphological index of the artery, down-regulate the collagen volume fraction in the media and inhibit the transformation of smooth muscle cells. However, the detailed mechanisms underlying its effects require further investigation, which might provide more scientific evidence for the clinical treatment of hypertensive vascular remodeling (VR). Objective: We investigated the effects of QC-containing serum on the TGF-b1/ERK signaling pathway, cell proliferation, migration, the cell cycle, apoptosis and matrix metalloproteinase synthesis (MMPs) in rat aortic adventitial fibroblasts (AFs). Materials and methods: AFs were cultured through tissue explants in vitro. The levels of extracellular signal-regulated kinase 1/2 (ERK1/2), phospho-ERK1/2 (p-ERK1/2), connective tissue growth factor (CTGF), MMP2 and MMP9 expression were measured by western blotting and RT-PCR. The proliferation and migration of AFs were measured by MTT and transwell migration assays. Cell cycle progression and apoptosis in AFs were analyzed by flow cytometry. Results:The proliferation and migration rates of AFs treated with transforming growth factor b1 (TGF-b1) for 24 h were 2.4 AE 0.75 and 2.2 AE 0.06 times higher than those of untreated AFs, and increases in the expression of p-ERK1/2 (3.7 AE 0.15 times), CTGF (3.3 AE 0.24 times), MMP2 (5.7 AE 0.37 times) and MMP9 (5.4 AE 0.46 times) (p50.05) were observed. Treatment with QC-containing serum significantly down-regulated cell proliferation (1.9 AE 0.06 times), migration (1.6 AE 0.05 times) and the expression of p-ERK1/2 (1.3 AE 0.75 times), CTGF (1.8 AE 0.64 times), MMP2 (1.6 AE 0.65 times) and MMP9 (1.4 AE 0.46 times) (p50.05). We also found that QC-containing serum down-regulated the percentage of cells in the G1 phase by 1.6 AE 0.43 times and increased early-phase apoptosis by 2.3 AE 0.33 times (p50.05) in AFs. Conclusions: QC effectively inhibits the proliferation and migration of AFs and changes cell bioactivity and MMPs, possibly through the TGF-b/ERK/CTGF signaling pathway. Our findings may provide new insights into the potential function of QC in preventing or treating hypertension.

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Section: Measurement Of Cell Proliferationmentioning

confidence: 99%

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

Lu¹,

Liu²,

Yuan³

et al. 2013

Pharmaceutical Biology

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“…These finding appear to contradict the physical similarities between the mass energy absorption coefficients of gold and soft tissue using MV radiation sources. Despite this numerous groups have reported significant DEFs using clinical radiation sources delivering MV X-rays (5,(43)(44)(45). The data presented by…”

Section: Gold Nanoparticles (Z=79)mentioning

confidence: 99%

Radiosensitising Nanoparticles as Novel Cancer Therapeutics — Pipe Dream or Realistic Prospect?

et al. 2013

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The field of high atomic number nanoparticle radiosensitising agents is reviewed. After a brief discussion of the new mode of physicochemical action implied by irradiation of high atomic number nanoparticles embedded in biological systems, a series of exemplars are discussed.Silver-, gallium-and gold-based nanoparticles are discussed in order of increasing atomic number with functionalization strategies being outlined. In-vitro and in-vivo evidence for radioenhancement and the mechanisms attributed to the increased biological effect are discussed.

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“…In our previous study, we have validated that PEG-Glu-GNPs can enhance the irradiation treatment of solid tumor cells, such as breast cancers, 9 prostate cancers, 30 and ovarian cancer. 11 To compare the therapeutic efficacy of Glu-GNPs with different types of cells, THP-1 and MCF-7 cells were chosen. Note that the breast cancer cell line MCF-7 was selected because it is a commonly used cancer cell line in cancer studies.…”

Section: Characterization Of Nanoparticles and Cellular Uptakementioning

confidence: 99%

“…Many studies have shown that functional nanoparticles are more effective in treating cancers and improving cellular targeting and X-ray irradiation sensitization than nanoparticles alone. 7,[9][10][11] Among these nanoparticles, biocompatible GNPs are the only US Food and Drug Administration (FDA) approved nanomaterials for clinical trials. 12 Many researchers have also shown that GNPs are promising candidates for various biomedical applications in cancer diagnostics and therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Treating cancer stem cells and cancer metastasis using glucose-coated gold nanoparticles

Hu¹,

Niestroj²,

Yuan³

et al. 2015

IJN

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Cancer ranks among the leading causes of human mortality. Cancer becomes intractable when it spreads from the primary tumor site to various organs (such as bone, lung, liver, and then brain). Unlike solid tumor cells, cancer stem cells and metastatic cancer cells grow in a non-attached (suspension) form when moving from their source to other locations in the body. Due to the non-attached growth nature, metastasis is often first detected in the circulatory systems, for instance in a lymph node near the primary tumor. Cancer research over the past several decades has primarily focused on treating solid tumors, but targeted therapy to treat cancer stem cells and cancer metastasis has yet to be developed. Because cancers undergo faster metabolism and consume more glucose than normal cells, glucose was chosen in this study as a reagent to target cancer cells. In particular, by covalently binding gold nanoparticles (GNPs) with thio-PEG (polyethylene glycol) and thio-glucose, the resulting functionalized GNPs (Glu-GNPs) were created for targeted treatment of cancer metastasis and cancer stem cells. Suspension cancer cell THP-1 (human monocytic cell line derived from acute monocytic leukemia patients) was selected because it has properties similar to cancer stem cells and has been used as a metastatic cancer cell model for in vitro studies. To take advantage of cancer cells’ elevated glucose consumption over normal cells, different starvation periods were screened in order to achieve optimal treatment effects. Cancer cells were then fed using Glu-GNPs followed by X-ray irradiation treatment. For comparison, solid tumor MCF-7 cells (breast cancer cell line) were studied as well. Our irradiation experimental results show that Glu-GNPs are better irradiation sensitizers to treat THP-1 cells than MCF-7 cells, or Glu-GNPs enhance the cancer killing of THP-1 cells 20% more than X-ray irradiation alone and GNP treatment alone. This finding can help oncologists to design therapeutic strategies to target cancer stem cells and cancer metastasis.

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Thio-glucose bound gold nanoparticles enhance radio-cytotoxic targeting of ovarian cancer

Cited by 155 publications

References 23 publications

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts

Radiosensitising Nanoparticles as Novel Cancer Therapeutics — Pipe Dream or Realistic Prospect?

Treating cancer stem cells and cancer metastasis using glucose-coated gold nanoparticles

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