Thioacidolysis

Rolando, Christian; Monties, Bernard; Lapierre, Catherine

doi:10.1007/978-3-642-74065-7_23

Cited by 226 publications

(218 citation statements)

References 8 publications

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“…The acid-insoluble lignin (Klason lignin) was then calculating by subtracting the ash content from the acid-insoluble residue. The lignin composition was investigated via thioacidolysis similar to Rolando et al (1992), but on 10 mg of CWR and a 1-mL reaction mixture (0.025 mL of boron trifluoride etherate + 0.1 mL of ethanethiol + 0.875 mL of dioxane).…”

Section: Lignin and Ash Analysismentioning

confidence: 99%

Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

et al. 2016

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Lignin is a phenolic heteropolymer that is deposited in secondary-thickened cell walls, where it provides mechanical strength. A recent structural characterization of cell walls from monocot species showed that the flavone tricin is part of the native lignin polymer, where it is hypothesized to initiate lignin chains. In this study, we investigated the consequences of altered tricin levels on lignin structure and cell wall recalcitrance by phenolic profiling, nuclear magnetic resonance, and saccharification assays of the naturally silenced maize (Zea mays) C2-Idf (inhibitor diffuse) mutant, defective in the CHALCONE SYNTHASE Colorless2 (C2) gene. We show that the C2-Idf mutant produces highly reduced levels of apigenin-and tricin-related flavonoids, resulting in a strongly reduced incorporation of tricin into the lignin polymer. Moreover, the lignin was enriched in b-b and b-5 units, lending support to the contention that tricin acts to initiate lignin chains and that, in the absence of tricin, more monolignol dimerization reactions occur. In addition, the C2-Idf mutation resulted in strikingly higher Klason lignin levels in the leaves. As a consequence, the leaves of C2-Idf mutants had significantly reduced saccharification efficiencies compared with those of control plants. These findings are instructive for lignin engineering strategies to improve biomass processing and biochemical production.

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Section: Lignin and Ash Analysismentioning

confidence: 99%

Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

et al. 2016

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“…Thioacidolysis was performed on 20 mg of extracted milled wood in 10 mL of reagent according to the method published by Rolando et al (1992). The reagent was prepared by addition of 2.5 mL of BF 3 etherate (Aldrich) and 10 mL of ethanethiol EtSH (Aldrich) to a 100 mL flask and adjusting the final volume to 100 mL with dioxane.…”

Section: Thioacidolysismentioning

confidence: 99%

Determination of Differences in Anatomical and Chemical Characteristics of Tension and Opposite Wood of 8-Year Old Eucalyptus Globulus

Aguayo

Quintupill

Castillo

et al. 2010

Maderas, Cienc. tecnol.

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Tension and opposite wood, divided in the corresponding heartwood and sapwood regions, from 8-year old Eucalyptus globulus trees were characterized for chemical composition and fibre anatomy in the transverse section in order to identify the main differences. Thicker and poorly lignified cell walls were found in tension wood. Cellulose content was similar for both woods, although tension wood contained 30% higher xylose and 15% less lignin than opposite wood. Lignin of tension wood contained 24% more syringyl units and a higher frequency of β-O-4 linkages. Principal component analysis (PCA) performed separately with anatomical and chemical data showed a clear separation between tension and opposite wood. The principal characteristics used for separation were: cell wall thickness, vessel diameter, lumen diameter, lignin and xylose content, and amount of syringyl units.

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“…Residual wood following methanolic extraction was subjected to lignin degradation and solubilization by thioacidolysis (Rolando et al, 1992). A 100-mL aliquot of solution containing degradation products was sampled and mixed in 3 mL of Fisher ScintiVerse scintillation cocktail.…”

Section: Thioacidolysismentioning

confidence: 99%

Tracking Monolignols during Wood Development in Lodgepole Pine

Kaneda

Rensing²,

Wong³

et al. 2008

Plant Physiology

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Secondary xylem (wood) formation in gymnosperms requires that the tracheid protoplasts first build an elaborate secondary cell wall from an array of polysaccharides and then reinforce it with lignin, an amorphous, three-dimensional product of the random radical coupling of monolignols. The objective of this study was to track the spatial distribution of monolignols during development as they move from symplasm to apoplasm. This was done by feeding [ 3 H]phenylalanine ([ 3 H]Phe) to dissected cambium/ developing wood from lodgepole pine (Pinus contorta var latifolia) seedlings, allowing uptake and metabolism, then rapidly freezing the cells and performing autoradiography to detect the locations of the monolignols responsible for lignification. Parallel experiments showed that radioactivity was incorporated into polymeric lignin and a methanol-soluble pool that was characterized by high-performance liquid chromatography. [ 3 H]Phe was incorporated into expected lignin precursors, such as coniferyl alcohol and p-coumaryl alcohol, as well as pinoresinol. Coniferin, the glucoside of coniferyl alcohol, was detected by high-performance liquid chromatography but was not radioactively labeled. With light microscopy, radiolabeled phenylpropanoids were detected in the rays as well as the tracheids, with the two cell types showing differential sensitivity to inhibitors of protein translation and phenylpropanoid metabolism. Secondary cell walls of developing tracheids were heavily labeled when incubated with [ 3 H]Phe. Inside the cell, cytoplasm was most strongly labeled followed by Golgi and low-vacuole label. Inhibitor studies suggest that the Golgi signal could be attributed to protein, rather than phenylpropanoid, origins. These data, produced with the best microscopy tools that are available today, support a model in which unknown membrane transporters, rather than Golgi vesicles, export monolignols.

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Thioacidolysis

Cited by 226 publications

References 8 publications

Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

Determination of Differences in Anatomical and Chemical Characteristics of Tension and Opposite Wood of 8-Year Old Eucalyptus Globulus

Tracking Monolignols during Wood Development in Lodgepole Pine

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