Thiobarbituric acid reaction of aldehydes and oxidized lipids in glacial acetic acid

Kosugi, Hiroko; Kikugawa, Kiyomi

doi:10.1007/bf02534777

Cited by 134 publications

(63 citation statements)

References 19 publications

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“…TBAreactive substances including aldehydes and MDA were assayed using the TBA test (Kosugi and Kikugawa, 1985), as modified by Meir et al (1992). One milliliter of 0.67% TBA was added to 3-mL aliquots of the supernatant and the solution was incubated at 100°C for 1 h. The solution was then cooled and centrifuged for 10 min at 8000 rpm.…”

Section: Extraction and Determination Of Lipid Peroxidation Productsmentioning

confidence: 99%

Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence

et al. 1997

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l h e ability to maintain the cytoplasmic Ca2+ concentration ([Ca2+],,,) at homeostatic levels has been examined during leaf senescence in detached parsley (Petroselinum crispum) leaves. Fluorescence ratiometric imaging of mesophyll cells isolated from parsley leaves at various senescence stages and loaded with the CaZ+ indicator fura-2 has revealed a distinct elevation of [Ca2+lCyl, which was positively correlated with the progress of leaf senescence. This initial increase of [Caz+lcyy which was first observed in cells iso- Senescence is a deteriorative process that is associated with a wide range of biochemical changes. Most notable in green leaves is the decrease of Chl content, which is accompanied by a parallel degradation of polar lipids from the matrix of the thylakoid membrane (Thomas and Stoddart, 1980;Thomas, 1986;Meir and Philosoph-Hadas, 1995) and by proteolysis (Thimann, 1985;Philosoph-Hadas et al., 1994). One of the factors that may participate in the regulation of leaf senescence is [Ca2+C],yt (Leshem, 1987), the regulation of which is considered an essential cell function in a11 eukaryotes (Bush, 1995). Cellular Ca2+ homeostasis is normally maintained at submicromolar levels by an ensemble of membrane-

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Section: Extraction and Determination Of Lipid Peroxidation Productsmentioning

confidence: 99%

Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence

et al. 1997

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“…The GR activity was calculated using the extinction coefficient of 6.12 mM −1 cm −1 (Halliwell and Foyer 1978). Malondialdehyde (MDA) content, an indicator of ROS was analyzed as previously described (Kosugi and Kikugawa 1985). Briefly, harvested tissues were ground in 20% trichloroacetic acid and 0.5% 2-thiobarbituric acid.…”

Section: Enzymatic Assaymentioning

confidence: 99%

“…The absorbance of the supernatant was subsequently recorded at 532 and 600 nm in a spectrophotometer. The MDA concentration was determined by subtracting the nonspecific turbidity at 600 nm by its molar extinction coefficient (Kosugi and Kikugawa 1985).…”

Section: Enzymatic Assaymentioning

confidence: 99%

Biochemical and molecular mechanisms associated with Zn deficiency tolerance and signaling in rice (Oryza sativa L.)

Kabir

Hossain

Khatun

et al. 2017

Journal of Plant Interactions

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In this study, zinc (Zn) deficiency caused a significant reduction in growth parameters and tissue Zn concentrations in BRRI 33 (sensitive) but not in Pokkali (tolerant). The increase of proton extrusion in both genotypes under high pH suggests that it gets triggered as a common consequence of reducing pH and solubilization of Zn. Real-time PCR showed pronounced upregulation of OsZIP4, OsDMAS1, OsNAS2 and OsPCS1 in Zn-deficient roots of Pokkali, and to a lesser extent in BRRI 33 only for OsZIP4 and OsPCS1. This suggests that OsDMAS1, OsNAS2 and OsPCS1 functions as secondary consequences leading to higher chelation and uptake of Zn under Zn deficiency in Pokkali. Further, a major increase in CAT, POD, SOD, GR and key metabolites suggests that high antioxidant defense plays a critical role in Zn deficiency tolerance in Pokkali. Further, Pokkali selfgrafts and plants having Pokkali rootstock combined with BRRI 33 scion showed no significant decline in plant height, root dry matter and Zn concentration along with upregulation of Zn transporters (OsZIP4 and OsIRT1) under Zn deficiency, suggesting that signal driving mechanisms for Zn deficiency tolerance mechanisms are generated in the root and Zn-inefficient BRRI 33 is not capable of producing signals or sensing them. ARTICLE HISTORY

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“…The cooled mixture was centrifuged at 13 000 g at 25 °C for 10 min and the absorbances of the supernatant were read at 532 and 600 nm. After subtracting the value at 600 nm, the MDA content was determined using the coefficient of absorbance of 155 mM -1 cm -1 (Kosugi and Kikugawa 1985).…”

Section: Western Blot Analysismentioning

confidence: 99%

Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

Chiang¹,

Chien²,

Chen³

et al. 2015

Biologia plant.

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Previously, the ascorbate peroxidase (APX1) activity and gene expression in Chinese cabbage (Brassica campestris, Bc) heat-tolerant cv. ASVEG2 were found to be significantly higher than in heat-sensitive cv. RN720 under a heat stress. Furthermore, BcAPX2 and BcAPX3, isoforms of BcAPX1, were cloned in this study. Our objective was to transfer BcAPX cDNA under the control of the ubiquitin promoter to Arabidopsis via Agrobacterium tumefaciens strain GV3101. We found that BcAPX genes were overexpressed in transgenic Arabidopsis, and the expression of APX, and the APX activity in transgenic lines were higher than in non-transgenic (NT) plants under high temperatures. The chlorophyll content and the germination rate were significantly higher, and the malondialdehyde content was lower in BcAPX1-3, 2-1, and 3-5 lines subjected to the heat-stress treatment than those in the NT plants. Compared to the NT plants, a lower heat-induced H 2 O 2 accumulation was detected by diaminobenzidine staining in leaves of the transgenic lines with a high APX activity indicating that the overexpression of BcAPX in Arabidopsis could enhance heat tolerance by eliminating H 2 O 2 .

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Thiobarbituric acid reaction of aldehydes and oxidized lipids in glacial acetic acid

Cited by 134 publications

References 19 publications

Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence

Increases in Cytosolic Ca2+ in Parsley Mesophyll Cells Correlate with Leaf Senescence

Biochemical and molecular mechanisms associated with Zn deficiency tolerance and signaling in rice (Oryza sativa L.)

Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

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