Thioclava pacifica gen. nov., sp. nov., a novel facultatively autotrophic, marine, sulfur-oxidizing bacterium from a near-shore sulfidic hydrothermal area

Sorokin, Dimitry Y.; Tourova, T. P.; Spiridonova, Elizaveta M.; Rainey, Fred A.; Muyzer, Gerard

doi:10.1099/ijs.0.63415-0

Cited by 74 publications

(39 citation statements)

References 19 publications

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“…No RuBisCo expression could be detected in any compartment other than the halocline, from which a strong positive signal was obtained by RT-PCR amplification of total RNA. As shown in Figure 7, phylogenetic analyses of the clone sequences revealed the presence of two distinct groups with a predominance of sequences corresponding to the CbbL type A subunit, a protein similar to the large RuBisCo subunit of Thioclava pacifica, a marine facultatively autotrophic, sulfur-oxidizing bacterium isolated from a near-shore sulfidic hydrothermal area (Sorokin et al, 2005). Sequences corresponding to the L'Atalante CbbL type B subunit formed a deep branch within the cluster of RuBisCo enzymes found in chemoautotrophic symbionts of various hydrothermal vent mussels (Schwedock et al, 2004).…”

Section: Diversity Of Prokaryotic Ssu Rrnasmentioning

confidence: 99%

Primary producing prokaryotic communities of brine, interface and seawater above the halocline of deep anoxic lake L'Atalante, Eastern Mediterranean Sea

et al. 2007

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Meso-and bathypelagic ecosystems represent the most common marine ecological niche on Earth and contain complex communities of microorganisms that are for the most part ecophysiologically poorly characterized. Gradients of physico-chemical factors (for example, depth-related gradients of light, temperature, salinity, nutrients and pressure) constitute major forces shaping ecosystems at activity 'hot spots' on the ocean floor, such as hydrothermal vents, cold seepages and mud volcanoes and hypersaline lakes, though the relationships between community composition, activities and environmental parameters remain largely elusive. We report here results of a detailed study of primary producing microbial communities in the deep Eastern Mediterranean Sea. The brine column of the deep anoxic hypersaline brine lake, L'Atalante, the overlying water column and the brine-seawater interface, were characterized physico-and geochemically, and microbiologically, in terms of their microbial community compositions, functional gene distributions and [ 14 C]bicarbonate assimilation activities. The depth distribution of genes encoding the crenarchaeal ammonia monooxygenase a subunit (amoA), and the bacterial ribulose-1,5-biphosphate carboxylase/ oxygenase large subunit (RuBisCO), was found to coincide with two different types of chemoautotrophy. Meso-and bathypelagic microbial communities were enriched in ammoniaoxidizing Crenarchaeota, whereas the autotrophic community at the oxic/anoxic interface of L'Atalante lake was dominated by Epsilonproteobacteria and sulfur-oxidizing Gammaproteobacteria. These autotrophic microbes are thus the basis of the food webs populating these deep-sea ecosystems.

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Section: Diversity Of Prokaryotic Ssu Rrnasmentioning

confidence: 99%

Primary producing prokaryotic communities of brine, interface and seawater above the halocline of deep anoxic lake L'Atalante, Eastern Mediterranean Sea

et al. 2007

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“…Most important from an ecological point of view is the degradation of the algal osmolyte demethylsulfoniopropionate (DMSP) through both the cleavage and the demethylation/dethiolation pathway, yielding the climate relevant gas dimethylsulfide (DMS) as well as carbon and sulfur compounds for incorporation into microbial biomass (Fig.3). In addition, sulfur-based lithoheterotrophy has been discovered in several Roseobacter strains (98,99) and the genome of Silicibacter pomeroyi (69). Roseobacter strains are also involved in transformations of DMS (35), methanethiol, methanesulfonate (46), and DMSO (70).…”

Section: Role Of the Roseobacter Lineage For Sulphur Cycling In The Omentioning

confidence: 99%

Environmental Biology of the Marine Roseobacter Lineage

Wagner‐Döbler¹,

Biebl²

2006

Annu. Rev. Microbiol.

435

363

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“…Many metabolically and phylogenetically diverse organisms involved in sulfur cycling have been isolated in pure culture from these environments and characterized in detail (6,10,32,36). However, the same cannot be said for sulfur-oxidizing chemolithotrophs in permanently cold environments.…”

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confidence: 99%

Isolation, Characterization, and Ecology of Cold-Active, Chemolithotrophic, Sulfur-Oxidizing Bacteria from Perennially Ice-Covered Lake Fryxell, Antarctica

Sattley

Madigan

2006

Appl Environ Microbiol

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Novel strains of obligately chemolithoautotrophic, sulfur-oxidizing bacteria have been isolated from various depths of Lake Fryxell, Antarctica. Physiological, morphological, and phylogenetic analyses showed these strains to be related to mesophilic Thiobacillus species, such as T. thioparus. However, the psychrotolerant Antarctic isolates showed an adaptation to cold temperatures and thus should be active in the nearly freezing waters of the lake. Enumeration by most-probable-number analysis in an oxic, thiosulfate-containing medium revealed that the sulfur-oxidizing chemolithotroph population peaks precisely at the oxycline (9.5 m), although viable cells exist well into the anoxic, sulfidic waters of the lake. The sulfur-oxidizing bacteria described here likely play a key role in the biogeochemical cycling of carbon and sulfur in Lake Fryxell.The role of sulfur-oxidizing bacteria (SOB) in carbon and sulfur cycling has been well studied in both temperate and geothermal aquatic environments (1,17,29). Many metabolically and phylogenetically diverse organisms involved in sulfur cycling have been isolated in pure culture from these environments and characterized in detail (6,10,32,36). However, the same cannot be said for sulfur-oxidizing chemolithotrophs in permanently cold environments.Although SOB have been identified in cold environments (25, 37), well-characterized pure cultures of these organisms are rare. One exception is in the work of Knittel et al. (19), in which two psychrophilic Thiomicrospira species, T. arctica and T. psychrophila, from sediments off the coast of Svalbard were described. These organisms were the first psychrophilic, chemolithoautotrophic, sulfur-oxidizing bacteria isolated in pure culture. In contrast to those studies, which focused on new species of SOB from marine sediments, our work describes planktonic SOB from the water column of the permanently ice-covered Lake Fryxell, Antarctica.Lake Fryxell is one of the most productive of several lakes in the McMurdo Dry Valleys of southern Victoria Land, Antarctica (41). The lake is just over 18 m deep and has a length of about 5.5 km, a width of 2 km, and a surface area of approximately 7 km 2 (21). A salinity gradient ranging from freshwater to about 0.8% NaCl near the sediments is also present in the lake (21). In addition to the increasing density of the water with depth, a thick ice cover that is currently just over 6 m contributes to a highly stratified and amictic water column (27,41). Water directly beneath the ice cover is supersaturated with dissolved gases, including oxygen and carbon dioxide (31). However, dissolved oxygen levels decrease dramatically below 8 m, and the water column becomes anoxic below 10 m, soon after the appearance of sulfide at 9.5 m (15). The sulfide, a product of sulfate-reducing bacteria, diffuses up from the sediments and anoxic portions of the water column, with concentrations increasing with depth to more than 1 mM near the sediments (13).The gradients of oxygen and sulfide in Lake Fryxell should prov...

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Thioclava pacifica gen. nov., sp. nov., a novel facultatively autotrophic, marine, sulfur-oxidizing bacterium from a near-shore sulfidic hydrothermal area

Abstract: Thioclava pacifica gen. nov., sp. nov., a novel facultatively autotrophic, marine, sulfur-oxidizing bacterium from a near-shore sulfidic hydrothermal area

Cited by 74 publications

References 19 publications

Primary producing prokaryotic communities of brine, interface and seawater above the halocline of deep anoxic lake L'Atalante, Eastern Mediterranean Sea

Primary producing prokaryotic communities of brine, interface and seawater above the halocline of deep anoxic lake L'Atalante, Eastern Mediterranean Sea

Environmental Biology of the Marine Roseobacter Lineage

Isolation, Characterization, and Ecology of Cold-Active, Chemolithotrophic, Sulfur-Oxidizing Bacteria from Perennially Ice-Covered Lake Fryxell, Antarctica

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