Thiol-chromene click chemistry: A turn-on fluorescent probe for specific detection of cysteine and its application in bioimaging

Thiol nucleophilic reactions and their applications in thiol fluorescent recognition

“…生物硫醇由于其结构的相似性, 前述色烯分子 对 3 种生物硫醇均显示了类似的光学响应. 为了开发 能够对单一生物硫醇的特异性识别的色烯分子, 我 们设计合成了化合物 4 [33] . 探针 [34,35] .…”

“…N-甲基马来酰亚胺(NMM)和 N-乙基马来酰亚胺(NEM)作为高效的硫醇捕获剂, 现 已广泛应用作细胞内源性硫醇的清除剂 [37,38] . 马来 图 5 特异性识别 Cys 的色烯衍生物 4 及其生物成像应用 [33] . 基于方酸为活性中心的探针 12 在 645 nm 处有强 的紫外-可见光吸收, 巯基对其的亲核加成过程打破 了其原有的共轭体系(图 10), 体系颜色逐渐由绿色变 为无色 [45] .…”

Highly Efficient Synthesis of New 2H-Chromene Dyes using Cu-SBA-15

“…Some of them are insecticide compounds 8 . In the recent years, they have been used in sensors for detection of metals and some compounds with high selectivity 9,10 . Until now, diverse methods have been published for the synthesis of 2H-benzopyran derivatives [11][12][13][14][15][16][17] .…”

A hydrophilicity-based fluorescent strategy to differentiate cysteine/homocysteine over glutathione both in vivo and in vitro

“…A number of them could only be operated in mixed solvents due to the poor water-solubility. 35,36 Moreover, the photobleaching under continual irradiation is still a problem for most indicators.…”