Thiram-induced cytotoxicity and oxidative stress in human erythrocytes: an in vitro study

Salam, Samreen; Arif, Amin; Mahmood, Riaz

doi:10.1016/j.pestbp.2019.12.003

Cited by 33 publications

(14 citation statements)

References 77 publications

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“…Moreover, the released Hb during hemolysis in intravascular space is toxic and induces numerous pathophysiologic effects (Schaer and Buehler, 2013). Anemia was resulted by increased hemolysis, as mentioned by (Arif et al, 2020;Salam et al, 2020) in human erythrocytes treated with bioallethrin and thiram, respectively.…”

Section: Discussionmentioning

confidence: 98%

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Hamed

Mosallam

Abdel-Razik

2022

Alexandria Science Exchange Journal

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Erythrocytes are a suitable model to study the membrane oxidative injury resulted by different xenobiotic. An attempt was made to study the antioxidant property of carvone (CAR), a monoterpene presents in herbs to mitigate toxicity of cypermethrin (CYP), a type II pyrethroid, in male rat erythrocytes in vitro. Erythrocytes were prepared and exposed to CYP (25-800 μM) alone and together with CAR (40 and 320 μM). Results showed superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were significantly decreaseed. Lipid peroxidation (LPO) level, reduced glutathione (GSH) content, both of glutathione stransfarase (GST), and glutathione reductase (GR) activities were significantly increased in comparison of control value. Scanning electron microscope (SEM) supported the toxic effect of CYP which presented various degrees of alteration in shape and ruptured membranes. Pre-treatment of erythrocytes with CAR induced alleviation in all the tested parameters. It can be recommended that CYP induced oxidative stress in vitro in rat erythrocytes and supplementation with CAR ameliorated these effects.

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Section: Discussionmentioning

confidence: 98%

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Hamed

Mosallam

Abdel-Razik

2022

Alexandria Science Exchange Journal

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“…For the obtention of plasma or serum, centrifugation and aspiration are needed. In addition, the preparation of lysate takes around one or two hours, depending on the chosen procedure [ 16 , 53 ]. In contrast, the preparation of WB samples is far more simple, since no centrifugation and washing procedures are needed.…”

Section: Discussionmentioning

confidence: 99%

Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs

et al. 2022

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The evaluation of the biomarkers of oxidative status is usually performed in serum, however, other samples, such as red blood cells (RBCs) lysates or whole blood (WB), can be used. The objective of this study was to evaluate if a comprehensive panel of redox biomarkers can be measured in the WB and RBCs of dogs, and their possible changes “in vitro” after the addition of different concentrations of ascorbic acid. The panel was integrated by biomarkers of the antioxidant status, such as cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type 1 (PON-1), and of the oxidant status, such as total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). All the assays were precise and accurate in WB and RBCs lysates. In addition, they showed changes after ascorbic acid addition that are in line with previously published results, being WB more sensitive to detect these changes in our experimental conditions. In conclusion, the panel of assays used in this study can be measured in the WB and RBCs of the dog. In particular, the higher sensitivity to detect changes in our experimental conditions and its easier sample preparation makes WB a promising sample for the evaluation of redox status in dogs, with also potential applications to other animal species and humans.

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“…Dithiocarbamic acid and its derivatives, including TMTD, have indiscriminate (multifarious) effects and disrupt a variety of biochemical processes that involve enzymes containing copper or sulfhydryl groups in pathogenic organisms [12,40,41]. The cytotoxic effect of TMTD results from the oxidation of the thiol group of peptides and proteins by the TMTD disulfide group, resulting in the formation of complexes with metal ions or interactions with essential cellular molecules [42,43]. TMTD is metabolized in the organism to form carbon disulfide and dimethyldithiocarbamate, which are also cytotoxic [44].…”

Section: Discussionmentioning

confidence: 99%

“…TMTD is metabolized in the organism to form carbon disulfide and dimethyldithiocarbamate, which are also cytotoxic [44]. Their toxicity is due to the formation of reactive oxygen species under oxidative stress, peroxidation of membrane lipids, and mitochondrial dysfunction in cells [43,45].…”

Section: Discussionmentioning

confidence: 99%

The Fungicide Tetramethylthiuram Disulfide Negatively Affects Plant Cell Walls, Infection Thread Walls, and Symbiosomes in Pea (Pisum sativum L.) Symbiotic Nodules

Gorshkov

Tsyganova

Vorobiev

et al. 2020

Plants

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In Russia, tetramethylthiuram disulfide (TMTD) is a fungicide widely used in the cultivation of legumes, including the pea (Pisum sativum). Application of TMTD can negatively affect nodulation; nevertheless, its effect on the histological and ultrastructural organization of nodules has not previously been investigated. In this study, the effect of TMTD at three concentrations (0.4, 4, and 8 g/kg) on nodule development in three pea genotypes (laboratory lines Sprint-2 and SGE, and cultivar ‘Finale’) was examined. In SGE, TMTD at 0.4 g/kg reduced the nodule number and shoot and root fresh weights. Treatment with TMTD at 8 g/kg changed the nodule color from pink to green, indicative of nodule senescence. Light and transmission electron microscopy analyses revealed negative effects of TMTD on nodule structure in each genotype. ‘Finale’ was the most sensitive cultivar to TMTD and Sprint-2 was the most tolerant. The negative effects of TMTD on nodules included the appearance of a senescence zone, starch accumulation, swelling of cell walls accompanied by a loss of electron density, thickening of the infection thread walls, symbiosome fusion, and bacteroid degradation. These results demonstrate how TMTD adversely affects nodules in the pea and will be useful for developing strategies to optimize fungicide use on legume crops.

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Thiram-induced cytotoxicity and oxidative stress in human erythrocytes: an in vitro study

Cited by 33 publications

References 77 publications

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs

The Fungicide Tetramethylthiuram Disulfide Negatively Affects Plant Cell Walls, Infection Thread Walls, and Symbiosomes in Pea (Pisum sativum L.) Symbiotic Nodules

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