Thirteen-Week Repeated Dose Toxicity of Rice Bran Glycosphingolipid in Wistar Hannover (Galas) Rats

Abstract

-Rice bran glycosphingolipid (RBGSL), one of the glycosphingolipids (GSLs), has been widely used as a food additive, a base of cosmetics, and so on. As a part of the safety assessment of RBGSL, a 13-week repeated dose toxicity study was performed in Wistar Hannover (GALAS) rats. Male and female rats were divided into 4 groups consisting of 8 animals and were given 0, 60, 250, and 1000 mg/kg BW of RBGSL orally 5 times weekly for 13 weeks. During the experiment, no deaths were observed in any groups, and there were no remarkable changes in general appearance, body weight, food and water consumption, hematological and serum biochemical parameters, organ weight and histopathological findings between the control and treated groups. On the basis of these data, the no-observedadverse effect level (NOAEL) of RBGSL in Wistar Hannover rats was considered to be 1000 mg/kg BW/ day or more.

Diluted Isoflurane as a Suitable Alternative for Diethyl ether for Rat Anaesthesia in Regular Toxicology Studies

“…In spite of these data, ether is still used in animal anaesthesia and euthanasia in Japanese laboratories [10,15,21,23]. There is a huge amount of historical data from toxicology studies that were conducted under ether anaesthesia.…”

Fatty Acid Components of Hardy Kiwifruit (Actinidia arguta) as IL-4 Production Inhibitor

“…(Lee et al, 2006), Mangifera indica (Rivera et al, 2006) and Evodia rutaecarpa (Shin et al, 2007) have inhibitory activities on atopic diseases. However, these natural products have also been known to have side effects (Moto et al, 2004;Thakur et al, 2005;Rodeiro Original Article Biomol Ther 19(1), 126-133 (2011) www.biomolther.org www.biomolther.org al., 2006;Yang et al, 2006). Efforts had been made to fi nd more distinguished and non-toxic materials with anti-allergic activity than the existing known natural products.…”