The increased serum concentration of CD36 is significantly
associated
with atherosclerosis, insulin resistance, and diabetes mellitus. Currently,
there is no sensor system used for the detection of CD36 in the clinical
field. Therefore, there is a need to develop a sensor system for the
detection of CD36. The large surface area/volume ratio and controllable
surface conformation of electrospun nanofibers (ENs) make them highly
attractive for immunosensor applications. In the present study, PS/MWCNT-PAMAM
ENs were produced and used as an immobilization matrix of Anti-CD36.
Thus, the electrochemical behavior of the developed nanocomposite-based
ENs and their usage potential were investigated for immunosensor applications.
First, an oxidized multiwall carbon nanotube (MWCNT-OH) was synthesized
and modified with a polyamidoamine generation 3 (PAMAM G3) dendrimer.
The synthesized MWCNT-PAMAM nanocomposite was mixed with polystyrene
(PS) solutions at different ratios to produce bead-free, smooth, and
uniform PS/MWCNT-PAMAM ENs. PS/MWCNT-PAMAM ENs were accumulated on
a screen-printed carbon electrode (SPCE) using the electrospinning
technique. A biofunctional surface on the PS/MWCNT-PAMAM EN-coated
SPCE was created using carbodiimide chemistry by covalent immobilization
of Anti-CD36. The analytic performance characteristics of the developed
PS/MWCNT-PAMAM/Anti-CD36 immunosensor were determined by performing
electrochemical measurements in the presence of the CD36 protein.
The linear detection range was found to be from 5 to 40 ng/mL, and
the limit of detection was calculated as 3.94 ng/mL for CD36. The
developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor also displayed high
tolerance to interference substances, good repeatability, and high
recovery percent (recovery%) for artificial blood serum analysis.