Three-dimensional acoustic impedance mapping of cultured biological cells

Hozumi, Naohiro; Kobayashi, Kazuto

doi:10.1016/j.ultras.2019.105966

Cited by 23 publications

(15 citation statements)

References 12 publications

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“…High-contrast images of the subcutaneous tissue using SAM can help in the clinical diagnosis of hair thinning or loss treatment by analysing the sebaceous glands, microvessels, nerves, and follicles of the detailed layers of the scalp. In recent years, advanced techniques such as ultrasound impedance microscopy, ultrasound speed microscopy, and scanning acoustic gigahertz microscopy have been developed for industrial and medical applications to provide additional information on the viscoelastic, anisotropic, internal stress, and nonlinear properties (Anastasiadis and Zinin, 2018;Demirkana et al 2019;Hozumia et al 2019). In ultrasound impedance microscopy, a plastic plate is inserted between the transducer and the biosample to be probed.…”

Section: Methodsmentioning

confidence: 99%

Scanning acoustic microscopy for material evaluation

2020

Appl. Microsc.

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Scanning acoustic microscopy (SAM) or Acoustic Micro Imaging (AMI) is a powerful, non-destructive technique that can detect hidden defects in elastic and biological samples as well as non-transparent hard materials. By monitoring the internal features of a sample in three-dimensional integration, this technique can efficiently find physical defects such as cracks, voids, and delamination with high sensitivity. In recent years, advanced techniques such as ultrasound impedance microscopy, ultrasound speed microscopy, and scanning acoustic gigahertz microscopy have been developed for applications in industries and in the medical field to provide additional information on the internal stress, viscoelastic, and anisotropic, or nonlinear properties. X-ray, magnetic resonance, and infrared techniques are the other competitive and widely used methods. However, they have their own advantages and limitations owing to their inherent properties such as different light sources and sensors. This paper provides an overview of the principle of SAM and presents a few results to demonstrate the applications of modern acoustic imaging technology. A variety of inspection modes, such as vertical, horizontal, and diagonal cross-sections have been presented by employing the focus pathway and image reconstruction algorithm. Images have been reconstructed from the reflected echoes resulting from the change in the acoustic impedance at the interface of the material layers or defects. The results described in this paper indicate that the novel acoustic technology can expand the scope of SAM as a versatile diagnostic tool requiring less time and having a high efficiency.

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Section: Methodsmentioning

confidence: 99%

Scanning acoustic microscopy for material evaluation

2020

Appl. Microsc.

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“…However, the relative impedance contrast was chosen to be identical for cells and nuclei which poses a serious limitation particularly as it also can affect the nucleus/cell scattering ratio w term. A future improvement could be the addition of a direct impedance contrast assessment using acoustic microscopy, for example [24], [25].…”

Section: Limits Of the Studymentioning

confidence: 99%

Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering

Muleki-Seya

O’Brien

2022

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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The histologically identifiable cellular structure(s) involved in ultrasonic scattering is(are) yet to be uniquely identified. The study quantifies six possible cellular scattering parameters, namely, cell and nucleus radii and their respective cell and nucleus volume fractions as well as a combination of cell and nucleus radii and their volume fraction. The six cellular parameters are each derived from four cell lines (4T1, JC, LMTK and MAT) and two tissue types (cell-pellet biophantom and ex vivo tumor). Optical histology and quantitative ultrasound (QUS), both independent approaches, are used to yield these cellular parameters. QUS scatterer parameters are experimentally determined using two ultrasonic scattering models: the spherical Gaussian model (GM) and the structure factor model (SFM) to yield insight about scattering from nuclei only and cells only. GM is a classical ultrasonic scattering model to evaluate QUS parameters and is well adapted for diluted media. SFM is adapted for dense media to estimate reasonably well scatterer parameters of cellular structures from ex vivo tissue. Nucleus and cell radii and volume fractions are measured optically from histology. They were used as inputs to calculate BSC for scattering from cells, nuclei, and both cells and nuclei. The QUS-derived scatterers (radii, volume fractions) distributions were then compared to the optical histology scatterer parameters derived from these calculated BSCs. The results suggest scattering from cells only (LMTK and MAT) or both cells and nuclei (4T1 and JC) for cellpellet biophantoms and scattering from nuclei only for tumors.

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“…SAM as used in this study employed the measurement system described previously. [3][4][5][6][7][8][9][10] Briefly, the PS film dish containing the cell culture was placed above the transducer, which has a central frequency of 320 MHz [Fig. 1(a)], and the reflection signals were received by the same transducer.…”

Section: Scanning Acoustic Microscopymentioning

confidence: 99%

“…First, living observation is feasible as the acoustic wave generated from the transducer in order to map the cells is non-invasive. [3][4][5][6][7][8][9][10][11][12][13] Next, the dynamics of cellular morphology correspond with the cell elasticity and could give us new insight into the changes in cellular mechanical properties which are often overlooked. 6,[9][10][11]13,14) These benefits are unattainable with current techniques for studying breast cancer, which often involve invasive methods such as biopsy.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

Soon

Sasaki

Prastika

et al. 2022

Jpn. J. Appl. Phys.

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Scanning acoustic microscope (SAM) is a useful observational tool in cellular study as living observation which is non-invasive is feasible compared to conventional optical microscope. In previous study, the cell morphological change by acoustic impedance measurement were successfully visualized. These acoustic impedance changes correspond with cell elasticity mainly reflects the changes of the cytoskeleton. In this study, we evaluate the elastic change of murine breast cancer cell, C127I during mitosis. C127I cells were cultured to ~75% confluency before measurement, using a transducer with a central frequency of 320 MHz. Dynamic changes during mitosis were successfully mapped using SAM and confirmed by laser confocal microscopy. Cells in prometaphase, anaphase and telophase which can only be confirmed through immunostaining before were successfully visualized using SAM. This suggests that SAM is capable to distinguish cell in different mitotic phase based on the changes of acoustic impedance.

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Three-dimensional acoustic impedance mapping of cultured biological cells

Cited by 23 publications

References 12 publications

Scanning acoustic microscopy for material evaluation

Scanning acoustic microscopy for material evaluation

Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering

Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

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