Three-dimensional acoustic impedance mapping of human skin by improved time–frequency domain analysis

Prastika, Edo Bagus; Imori, Atsushi; Kawashima, T.; Murakami, Yoshinobu; Hozumi, Naohiro; Nagaoka, Ryo; Kobayashi, Kazuto

doi:10.35848/1347-4065/abf512

Cited by 6 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the relative impedance contrast was chosen to be identical for cells and nuclei which poses a serious limitation particularly as it also can affect the nucleus/cell scattering ratio w term. A future improvement could be the addition of a direct impedance contrast assessment using acoustic microscopy, for example [24], [25].…”

Section: Limits Of the Studymentioning

confidence: 99%

Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering

Muleki-Seya

O’Brien

2022

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

View full text Add to dashboard Cite

The histologically identifiable cellular structure(s) involved in ultrasonic scattering is(are) yet to be uniquely identified. The study quantifies six possible cellular scattering parameters, namely, cell and nucleus radii and their respective cell and nucleus volume fractions as well as a combination of cell and nucleus radii and their volume fraction. The six cellular parameters are each derived from four cell lines (4T1, JC, LMTK and MAT) and two tissue types (cell-pellet biophantom and ex vivo tumor). Optical histology and quantitative ultrasound (QUS), both independent approaches, are used to yield these cellular parameters. QUS scatterer parameters are experimentally determined using two ultrasonic scattering models: the spherical Gaussian model (GM) and the structure factor model (SFM) to yield insight about scattering from nuclei only and cells only. GM is a classical ultrasonic scattering model to evaluate QUS parameters and is well adapted for diluted media. SFM is adapted for dense media to estimate reasonably well scatterer parameters of cellular structures from ex vivo tissue. Nucleus and cell radii and volume fractions are measured optically from histology. They were used as inputs to calculate BSC for scattering from cells, nuclei, and both cells and nuclei. The QUS-derived scatterers (radii, volume fractions) distributions were then compared to the optical histology scatterer parameters derived from these calculated BSCs. The results suggest scattering from cells only (LMTK and MAT) or both cells and nuclei (4T1 and JC) for cellpellet biophantoms and scattering from nuclei only for tumors.

show abstract

Section: Limits Of the Studymentioning

confidence: 99%

Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering

Muleki-Seya

O’Brien

2022

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

View full text Add to dashboard Cite

show abstract

“…Increasing the radiation frequency enhances the range and lateral resolution of the US, increasing the measurement accuracy and image quality. [1][2][3][4][5] For example, frequencies from several megahertz to several tens of megahertz are used for medical sonography and those from several tens of kilohertz to several hundred kilohertz are used in air. Absorption attenuation at high frequencies limits the penetration depth of US to near the surfaces of media and the strong scattering of high-frequency (HF) US by high-contrast targets such as bones in soft tissues makes medical diagnosis difficult.…”

Section: Introductionmentioning

confidence: 99%

Experimental evaluation of effects of absorption layer on parametric ultrasound

Nomura

Imaizumi

2023

Jpn. J. Appl. Phys.

View full text Add to dashboard Cite

This study investigated the effects of absorption attenuation on parametric ultrasound (US) for application to low-frequency (LF) US measurement and imaging. The effects of an absorption layer on the sound field and pulsed wave of parametric US at several hundred kilohertz in water were experimentally evaluated via a comparison with LF US directly radiated from a transmitter. The results indicate that the variations of the shape and amplitude of parametric US field and waveform due to the insertion of an absorber were more significant than those for LF US at the same frequency. However, these effects can be mitigated when an absorber is inserted at a far distance from a transmitter. These results suggest that for the application of parametric US in measurement and imaging, a low-absorption medium must be inserted between a transmitter and absorbing material to be evaluated in order to grow secondary waves sufficiently.

show abstract

“…In recent years, ultrasound technology has made remarkable progress in medical applications, and is often used for imaging, 1,2) biosensors, [3][4][5] and gas sensors, 6,7) and the ultrasound frequencies used in these applications are generally in the megahertz band. However, it has become clear that even low-frequency ultrasound waves with a frequency of several tens of kilohertz can interact with small proteins and dramatically affect their reactions as described below.…”

Section: Introductionmentioning

confidence: 99%

Acceleration of amyloid fibril formation by multichannel sonochemical reactor

Noi¹,

Nakajima²,

Yamaguchi³

et al. 2022

Jpn. J. Appl. Phys.

View full text Add to dashboard Cite

Formation of amyloid fibrils of various amyloidogenic proteins is dramatically enhanced by ultrasound irradiation. For applying this phenomenon to the study of protein aggregation science and diagnosis of neurodegenerative diseases, a multichannel ultrasound irradiation system with individually adjustable ultrasound-irradiation conditions is necessary. Here, we develop a sonochemical reaction system, where an ultrasonic transducer is placed in each well of a 96-well microplate to perform ultrasonic irradiation of sample solutions under various conditions with high reproducibility, and applied it for studying amyloid-fibril formation of amyloid $\beta$, $\alpha$-synuclein, $\beta$2-microglobulin, and lysozyme. The results clearly show that our instrument is superior to conventional shaking method in terms of degree of acceleration and reproducibility of fibril formation reaction. The acceleration degree is controllable by controlling the driving voltage applied to each transducer. We have thus succeeded in developing a useful tool for the study of amyloid fibril formation in various proteins.

show abstract

Three-dimensional acoustic impedance mapping of human skin by improved time–frequency domain analysis

Cited by 6 publications

References 32 publications

Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering

Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering

Experimental evaluation of effects of absorption layer on parametric ultrasound

Acceleration of amyloid fibril formation by multichannel sonochemical reactor

Contact Info

Product

Resources

About