Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine

Xie, Feng; Sun, Lejia; Pang, Yuan; Xu, Gang; Bao, Ji; Xu, Haifeng; Lu, Xin; Xu, Yiyao; Du, Shunda; Wang, Yanan; Feng, Shi Ting; Sang, Xinting; Zhong, Shouxian; Wang, Xin; Sun, Wei; Zhao, Hong; Zhang, Hongbing; Yang, Huayu; Huang, Pengyu; Mao, Yilei

doi:10.1016/j.biomaterials.2020.120416

Cited by 96 publications

(69 citation statements)

References 49 publications

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“…With the mass ratio of SC increasing from 1:1 to 16:1, the pore size in the cross section increased from tens of micrometers to hundreds of micrometers. The SC4:1 blend hydrogel membrane showed a proper size of pores, about 200-300 µm, for more effective cell adhesion [36][37][38][39] between two polymers, and the resulting spectrum will be greatly deviated from that of several groups of single-component polymers, and there will be certain displacement and changes in the main absorption bands, as well as changes in the position width or relative strength of the peak [40]. From Figure 3a, for pure SA and pure CMC-Na, the broad peak at 3451 cm −1 was caused by the absorption of hydroxyl group (-OH) stretching vibration, but -OH stretching vibration peak of the SC blend hydrogels was 3423 cm −1 .…”

Section: Morphologies Of Sc Blend Hydrogelsmentioning

confidence: 99%

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Zhang

Wang

Wei

et al. 2021

Gels

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Tissue-engineered skin grafts have long been considered to be the most effective treatment for large skin defects. Especially with the advent of 3D printing technology, the manufacture of artificial skin scaffold with complex shape and structure is becoming more convenient. However, the matrix material used as the bio-ink for 3D printing artificial skin is still a challenge. To address this issue, sodium alginate (SA)/carboxymethyl cellulose (CMC-Na) blend hydrogel was proposed to be the bio-ink for artificial skin fabrication, and SA/CMC-Na (SC) composite hydrogels at different compositions were investigated in terms of morphology, thermal properties, mechanical properties, and biological properties, so as to screen out the optimal composition ratio of SC for 3D printing artificial skin. Moreover, the designed SC composite hydrogel skin membranes were used for rabbit wound defeat repairing to evaluate the repair effect. Results show that SC4:1 blend hydrogel possesses the best mechanical properties, good moisturizing ability, proper degradation rate, and good biocompatibility, which is most suitable for 3D printing artificial skin. This research provides a process guidance for the design and fabrication of SA/CMC-Na composite artificial skin.

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Section: Morphologies Of Sc Blend Hydrogelsmentioning

confidence: 99%

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Zhang

Wang

Wei

et al. 2021

Gels

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show abstract

“…This method was further extended to generate HBV infected patient-derived models for HCC. The 3D bio-printed HCC survived well, retained the specific biomarkers of HCC, and maintained both genetic alterations and the expression profile of their original tumors [98].…”

Section: Organoids and Viral Infectionsmentioning

confidence: 90%

Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research

Duzagac

Saorin

Memeo³

et al. 2021

Cancers

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Organ-like cell clusters, so-called organoids, which exhibit self-organized and similar organ functionality as the tissue of origin, have provided a whole new level of bioinspiration for ex vivo systems. Microfluidic organoid or organs-on-a-chip platforms are a new group of micro-engineered promising models that recapitulate 3D tissue structure and physiology and combines several advantages of current in vivo and in vitro models. Microfluidics technology is used in numerous applications since it allows us to control and manipulate fluid flows with a high degree of accuracy. This system is an emerging tool for understanding disease development and progression, especially for personalized therapeutic strategies for cancer treatment, which provide well-grounded, cost-effective, powerful, fast, and reproducible results. In this review, we highlight how the organoid-on-a-chip models have improved the potential of efficiency and reproducibility of organoid cultures. More widely, we discuss current challenges and development on organoid culture systems together with microfluidic approaches and their limitations. Finally, we describe the recent progress and potential utilization in the organs-on-a-chip practice.

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“…Notably, in a recently published study, Xie et al . generated a patient-derived hepatocellular carcinoma (HCC) model using cell-laden gelatin/alginate as bio-ink[ 188 ]. Immunofluorescence staining revealed the stabilized expression of α-fetoprotein in HCC model over a 2-week culture period.…”

Section: Progress In Establishing 3d Tumor Models Via 3d Bioprintingmentioning

confidence: 99%

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

Zhuang¹,

Chiang²,

Fernanda³

et al. 2024

IJB

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Cancer still ranks as a leading cause of mortality worldwide. Although considerable efforts have been dedicated to anticancer therapeutics, progress is still slow, partially due to the absence of robust prediction models. Multicellular tumor spheroids, as a major three-dimensional (3D) culture model exhibiting features of avascular tumors, gained great popularity in pathophysiological studies and high throughput drug screening. However, limited control over cellular and structural organization is still the key challenge in achieving in vivo like tissue microenvironment. 3D bioprinting has made great strides toward tissue/organ mimicry, due to its outstanding spatial control through combining both cells and materials, scalability, and reproducibility. Prospectively, harnessing the power from both 3D bioprinting and multicellular spheroids would likely generate more faithful tumor models and advance our understanding on the mechanism of tumor progression. In this review, the emerging concept on using spheroids as a building block in 3D bioprinting for tumor modeling is illustrated. We begin by describing the context of the tumor microenvironment, followed by an introduction of various methodologies for tumor spheroid formation, with their specific merits and drawbacks. Thereafter, we present an overview of existing 3D printed tumor models using spheroids as a focus. We provide a compilation of the contemporary literature sources and summarize the overall advancements in technology and possibilities of using spheroids as building blocks in 3D printed tissue modeling, with a particular emphasis on tumor models. Future outlooks about the wonderous advancements of integrated 3D spheroidal printing conclude this review.

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Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine

Cited by 96 publications

References 49 publications

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research

Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

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