Three-Dimensional Biomimetic Mineralization of Dense Hydrogel Templates

Liu, Gao; Zhao, Dacheng; Tomsia, Antoni P.; Minor, Andrew M.; Song, Xiangyun; Saiz, Eduardo

doi:10.1021/ja903817z

Cited by 47 publications

(52 citation statements)

References 23 publications

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“…6B2, C2). These results are in accordance with those shown by Liu et al [40] who identified large mineral plates within a water-entrapped polymer hydrogel matrix corresponding to the basal region of the filled water channels and agglomerates of apatite nanocrystals within the gap region of the water channel. However, it is also possible that the crystallised SBFS-aged bonded-dentine interface created using resin A and B may have had similar mechanical characteristics as well as those created by GIC applied on PAA-etched dentine when submitted to tensile tests.…”

Section: Discussionsupporting

confidence: 93%

Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentine interface

Sauro

Osorio

Watson

et al. 2012

J Mater Sci: Mater Med

126

108

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This study aimed in evaluating the effects of two experimental resin bonding systems containing conventional Bioglass 45S5 (BAG) or Zinc-polycarboxylated bioactive glass (BAG-Zn) micro-fillers on the resin-bonded dentine interface after storage in a simulated body fluid solution (SBFS). Three resin bonding systems were formulated: Resin-A: (BAG containing); Resin-B; (BAG-Zn containing); Resin-C (no filler). The ability of the experimental resins to evoke apatite formation was evaluated using confocal Raman spectroscopy. Acid-etched dentine specimens were bonded, and prepared for AFM/nano-indentation analysis in a fully-hydrated status to evaluate the modulus of elasticity (Ei) and hardness (Hi) across the interface at different SBFS storage periods. Further resin-dentine specimens were tested for microtensile bond strength after 24 h or 3 months of SBFS storage. SEM examination was performed after de-bonding and confocal laser microscopy was used to evaluate the ultramorphology of the interfaces and micropermeability. The resin A and B showed a consistent presence of apatite (967 cm(-1)), reduced micropermeability within the resin-dentine interface and a significant increase of the Ei and Hi along the bonded-dentine interface after prolonged SBFS storage. Bond strength values were affected by the resin system (P < 0.0001) and by storage time (P < 0.0001) both after 24 h and 3 months of SBFS storage. In conclusion, resin bonding systems containing bioactive fillers may a have therapeutic effect on the nano-mechanical properties and sealing ability of mineral-depleted resin-dentine interface.

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Section: Discussionsupporting

confidence: 93%

Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentine interface

Sauro

Osorio

Watson

et al. 2012

J Mater Sci: Mater Med

126

108

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“…This is a particular problem when working with nanoporous, elastic hydrogels with limited water incorporation. The use of electrical assisted diffusion seems to be a path to promote ion diffusion and true 3D mineralization (Figure 5) [58, 59]. It has been observed that phase separation during the process can result in the formation of microscopic liquid vesicles in the organic matrix that will play a similar role to the vesicles secreted by osteoblasts during bone formation [59].…”

Section: Nanocomposite Approaches For Scaffoldsmentioning

confidence: 99%

Perspectives on the role of nanotechnology in bone tissue engineering

Saiz¹,

Zimmermann²,

Lee³

et al. 2013

Dental Materials

Self Cite

118

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Objective This review surveys new developments in bone tissue engineering, specifically focusing on the promising role of nanotechnology and describes future avenues of research. Methods The review first reinforces the need to fabricate scaffolds with multi-dimensional hierarchies for improved mechanical integrity. Next, new advances to promote bioactivity by manipulating the nano-level internal surfaces of scaffolds are examined followed by an evaluation of techniques to using scaffolds as a vehicle for local drug delivery to promote bone regeneration/integration and methods of seeding cells into the scaffold. Results Through a review of the state of the field, critical questions are posed to guide future research towards producing materials and therapies to bring state-of-the-art technology to clinical settings. Significance The development of scaffolds for bone regeneration requires a material able to promote rapid bone formation while possessing sufficient strength to prevent fracture under physiological loads. Success in simultaneously achieving mechanical integrity and sufficient bioactivity with a single material has been limited. However, the use of new tools to manipulate and characterize matter down to the nano-scale may enable a new generation of bone scaffolds that will surpass the performance of autologous bone implants.

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“…Here, the vesicles secrete apatitic crystals that subsequently calcify periodically arranged, calcium-binding hole zones with specific amino acid composition in collagen fibers of the extracellular matrix. 79,80 Liu et al 81 created liquid vesicles that entered the hydrogel matrix using a current-mediated ion diffusion method that resulted in mineralization at the interior of a pHEMA hydrogel. The dense hydrogel acted as binding site for the Ca ions and promoted mineralization of nanoapatite.…”

Section: Vesicles Loaded With Ca 2þ and Po 4 3àmentioning

confidence: 99%

Mineralization of Hydrogels for Bone Regeneration

Gkioni

Leeuwenburgh

Douglas

et al. 2010

Tissue Engineering Part B: Reviews

215

179

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Hydrogels are an important class of highly hydrated polymers that are widely investigated for potential use in soft tissue engineering. Generally, however, hydrogels lack the ability to mineralize, preventing the formation of chemical bonds with hard tissues such as bone. A recent trend in tissue engineering involves the development of hydrogels that possess the capacity to mineralize. The strategy that has attracted most interest has been the incorporation of inorganic phases such as calcium phosphate ceramics and bioglasses into hydrogel matrices. These inorganic particles act as nucleation sites that enable further mineralization, thus improving the mechanical properties of the composite material. A second route to create nucleation sites for calcification of hydrogels involves the use of features from the physiological mineralization process. Examples of these biomimetic mineralization strategies include (1) soaking of hydrogels in solutions that are saturated with respect to calcium phosphate, (2) incorporation of enzymes that catalyze deposition of bone mineral, and (3) incorporation of synthetic analogues to matrix vesicles that are the initial sites of biomineralization. Functionalization of the polymeric hydrogel backbone with negatively charged groups is a third mechanism to promote mineralization in otherwise inert hydrogels. This review summarizes the main strategies that have been developed in the past decade to calcify hydrogel matrices and render these hydrogels suitable for applications in bone regeneration.

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Three-Dimensional Biomimetic Mineralization of Dense Hydrogel Templates

Cited by 47 publications

References 23 publications

Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentine interface

Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentine interface

Perspectives on the role of nanotechnology in bone tissue engineering

Mineralization of Hydrogels for Bone Regeneration

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