Three-dimensional cell-printing of advanced renal tubular tissue analogue

Singh, Narendra K.; Han, Wonil; Nam, Sun Ah; Kim, Jin Won; Kim, Jae Yun; Kim, Yong Kyun; Cho, Dong‐Woo

doi:10.1016/j.biomaterials.2019.119734

Cited by 114 publications

(126 citation statements)

References 34 publications

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“…The latter has been addressed recently in valuable in vitro tools for drug screening and disease modeling, with proven apical-basal cell polarity, and active reabsorption and transepithelial secretion function. These models provided evidence for the fact that tubular 3D curvature and biomimetic ECM properties enhance kidney cell functionality [2,3,[5][6][7][8]. However, for the engineering of potentially implantable and durable kidney tubules, scaffolds must meet at least three criteria: they must be (1) small-sized and highly porous to increase the surface area, (2) freely accessible from the basolateral and luminal sides for rapid solute exchange and removal, and (3) flexible and yet strong enough to withstand intracorporeal forces such as pressure, tear and wear.…”

Section: Introductionmentioning

confidence: 88%

“…To allow for rapid exchange of solutes between blood and urine, the epithelial and endothelial BM should be virtually the only barriers between both cell types. To date, tubular tissue engineering mostly relies on non-porous, large diameter tubular scaffolds (Ø > 2 mm) for sufficient self-support, or smaller tubular lumens (Ø < 1 mm) surrounded by hydrogels to account for physiological functions, including vectorial transport [2][3][4][5][6]. The latter has been addressed recently in valuable in vitro tools for drug screening and disease modeling, with proven apical-basal cell polarity, and active reabsorption and transepithelial secretion function.…”

Section: Introductionmentioning

confidence: 99%

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Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance

Jansen

Kristen

et al. 2020

Preprint

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To advance the engineering of kidney tubules for future implantation, constructs should be both self-supportive and yet small-sized and highly porous. Here, we hypothesize that the fabrication of small-sized porous tubular scaffolds with a highly organized fibrous microstructure by means of melt-electrowriting (MEW) allows the development of self-supported kidney proximal tubules with enhanced properties. A custom-built MEW device was used to fabricate tubular fibrous scaffolds with small diameter sizes (Ø = 0.5, 1, 3 mm) and well-defined, porous microarchitectures (rhombus, square, and random). Human umbilical vein endothelial cells (HUVEC) and human conditionally immortalized proximal tubular epithelial cells (ciPTEC) were seeded into the scaffolds and tested for monolayer formation, integrity, and organization, as well as for extracellular matrix (ECM) production and renal transport functionality. Tubular scaffolds were successfully manufactured by fine control of MEW instrument parameters. A minimum inner diameter of 0.5 mm and pore sizes of 0.2 mm were achieved. CiPTEC formed tight monolayers in all scaffold microarchitectures tested, but well-defined rhombus-shaped pores outperformed and facilitated unidirectional cell orientation, increased collagen type IV deposition, and expression of the renal transporters and differentiation markers organic cation transporter 2 (OCT2) and P-glycoprotein (P-gp). To conclude, we present smaller diameter engineered kidney tubules with microgeometry-directed cell functionality. Due to the well-organized tubular fiber scaffold microstructure, the tubes are mechanically self-supported, and the self-produced ECM constitutes the only barrier between the inner and outer compartment, facilitating rapid and active solute transport.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance

Jansen

Kristen

et al. 2020

Preprint

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“…Not only that, 3D printing allows the simultaneous process of living cells and biomaterials, which provides tumor model with fine, replicating ECM on chips [35]. Using specific bio-ink formulations, 3D bioprinting is able to build different complex channels or ECM on chip and preserve the heterogeneity of the primary tumor [34,36]. In fact, for the organoid or biological scaffolds constructed by bio-printer, applying the mechanical force generated by fluid is able to simulate the metastasis of cancer cells in vivo.…”

Section: D Printed Microfluidic Chip and Cancer Modelsmentioning

confidence: 99%

Multifunctional microfluidic chip for cancer diagnosis and treatment

Guo¹,

Zhang²,

Liu³

et al. 2021

Nanotheranostics

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Microfluidic chip is not a chip in the traditional sense. It is technologies that control fluids at the micro level. As a burgeoning biochip, microfluidic chips integrate multiple disciplines, including physiology, pathology, cell biology, biophysics, engineering mechanics, mechanical design, materials science, and so on. The application of microfluidic chip has shown tremendous promise in the field of cancer therapy in the past three decades. Various types of cell and tissue cultures, including 2D cell culture, 3D cell culture and tissue organoid culture could be performed on microfluidic chips. Patient-derived cancer cells and tissues can be cultured on microfluidic chips in a visible, controllable, and high-throughput manner, which greatly advances the process of personalized medicine. Moreover, the functionality of microfluidic chip is greatly expanding due to the customizable nature. In this review, we introduce its application in developing cancer preclinical models, detecting cancer biomarkers, screening anti-cancer drugs, exploring tumor heterogeneity and producing nano-drugs. We highlight the functions and recent development of microfluidic chip to provide references for advancing cancer diagnosis and treatment.

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“…Singh et al developed a vascularized kidney-on-a-chip consisting of a renal proximal tubule and blood vessels. This was achieved using direct, coaxial cell-printing and a hybrid bioink made of kidney dECM and alginate [ 123 ]. The hybrid bioink improved the viability and the expression of functional markers such as gamma-glutamyl transpeptidase (GGT), aquaporin 1 (AQP1), and kidney-specific cadherin (KSP) in RPTECs compared to using medical-grade collagen type I bioink.…”

Section: Drug Screening Applicationsmentioning

confidence: 99%

“…The renal proximal tubular marker is presented in green, vascular endothelial markers are red, and nuclei are blue (scale bar: 100 μm); (iii) Albumin reabsorption between RTPEC and HUVEC tube via vectorial transport. Arrows indicate the intracellular accumulating albumin within the RPTEC and sidewall of the vessel (scale bar: 400 μm) (reproduced with permission from ref [ 123 ]; copyright 2020 Elsevier).…”

Section: Figurementioning

confidence: 99%

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

Kim

Kong

Han

et al. 2020

IJMS

Self Cite

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The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

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Three-dimensional cell-printing of advanced renal tubular tissue analogue

Cited by 114 publications

References 34 publications

Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance

Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance

Multifunctional microfluidic chip for cancer diagnosis and treatment

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

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