Three-Dimensional Co-Cultures of Osteoblasts and Endothelial Cells in DegraPol Foam: Histological and High-Field Magnetic Resonance Imaging Analyses of Pre-Engineered Capillary Networks in Bone Grafts

Buschmann, Johanna; Welti, Manfred; Hemmi, Sonja; Neuenschwander, Peter; Baltes, Christof; Giovanoli, Pietro; Rudin, Markus; Calcagni, Maurizio

doi:10.1089/ten.tea.2010.0278

Cited by 44 publications

(38 citation statements)

References 27 publications

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“…Insufficient vascularization after implantation of tissue-engineered constructs with MSCs leads to nutrient limitations, which then turn into cell death in the constructs [3,4]. Several strategies for improving the vascularization of tissue-engineered constructs have been studied extensively in recent years [5][6][7]. Among them, in vitro prevascularization of the 3-D tissue constructs using co-cultures of vascular endothelial cells (ECs) with bone-forming cells or MSCs has received much attention [8,9].…”

Section: Introductionmentioning

confidence: 99%

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Guerrero¹,

Catros²,

Derkaoui³

et al. 2013

Acta Biomaterialia

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Section: Introductionmentioning

confidence: 99%

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Guerrero¹,

Catros²,

Derkaoui³

et al. 2013

Acta Biomaterialia

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“…Nevertheless, a positive OB influence on EC organization in coculture seems to be a good reason to consider OB-EC coculture as a useful system in bone tissue engineering. 2,3,17,18 To add an extra value to such a system we put particular attention to the possible EC influence on OB proliferation in vitro. Expansion of normal cells originating from primary cultures, which are candidates for transplantation in tissue engineered systems, is not as effective as of immortalized cell lines derived from tumors, often used for basic research experiments.…”

mentioning

confidence: 99%

Contribution of Endothelial Cells to Human Bone-Derived Cells Expansion in Coculture

Leszczyńska

Żyżyńska-Granica

Koziak

et al. 2013

Tissue Engineering Part A

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Creating a functional vascularized bone tissue remains one of the main goals of bone tissue engineering. Recently, a growing interest in the crosstalk between endothelial cells (EC) and osteoblasts (OB), the two main players in a new bone formation, has been observed. However, only a few reports have addressed a mutual influence of OB and EC on cell proliferation. Our study focuses on this issue by investigating cocultures of human bone-derived cells (HBDC) and human umbilical vein endothelial cells (HUVEC). Three various proportions of cells have been used that is, HBDC:HUVEC 1:1, 1:4, and 4:1 and the cocultures were investigated on day 1, 4, and 7, while HUVEC and HBDC monocultures served as reference. We have detected enhanced alkaline phosphatase (ALP) activity in a direct HBDC-HUVEC coculture. This effect was not observed when cells were separated by an insert, which is consistent with other reports on various OB-EC lineages. The appearance of gap-junctions in coculture was confirmed by a positive staining for connexin 43. The number of cells of both phenotypes has been determined by flow cytometry: CD-31-positive cells have been considered EC, while CD-31-negative have been counted as OB. We have observed an over 14-fold increase in OB number after a week in the 1:4 HBDC:HUVEC coculture as compared with less than fourfold in monoculture. The increase in HBDC number in 1:1 coculture has been less pronounced and has reached the value of about sevenfold. These results correspond well with the cell proliferation rate, which has been measured by 5-bromo-2¢-deoxyuridine incorporation. Moreover, at day 7 EC have been still present in the coculture, which is inconsistent with some other reports. Real-time polymerase chain reaction analysis has revealed the upregulation of ALP and collagen type I genes, but not osteocalcin gene, in all the cocultures grown without pro-osteogenic additives. Our study indicates that HUVEC significantly promote HBDC expansion and upregulate collagen I gene expression in these cells. We believe that these findings have application potency in bone tissue engineering.

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“…This leads to creation of immature vessels which will mature and connect with the recipient vessels after implantation. This method leads to increased bone formation in vivo (1,4,6). The second option is based upon the implantation of the scaffold within the well vascularized tissue.…”

Section: Discussionmentioning

confidence: 99%

The Osteogenic Potential of Human Nondifferentiated and Pre-differentiated Mesenchymal Stem Cells Combined with an Osteoconductive Scaffold – Early Stage Healing

Tuček

Kočí

Karova

et al. 2017

Acta Med. (Hradec Kralove, Czech Repub.)

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A B ST R AC T Despite the huge research into stem cells and their regenerative properties for bone healing, there are still unanswered questions including the recipient's respond to the presence of the stem cells, the fate of stem cells inside the bone defect and the possible advantage in utilizingpre-differentiatedcells.Toaddresstheseproblems,weusedhumanmultipotentmesenchymalstromal/stemcells(MSCs),GMP Grade,inaratmodelofboneformation.Ina"bioreactorconcept"approachsevenWistarratswereimplantedwith0.2gofsynthetic bonescaffoldseededwith2×106MSCs,sevenWistarratswereimplantedwith0.2gofsyntheticbonescaffoldseededwith1×106pre-differentiatedosteoblastsand1×106pre-differentiatedendothelialcellsand14Wistarratswereimplantedwith0.2gofsyntheticbone scaffoldwithoutseededcellsintoanintramuscularpocketontheleftsideoftheirback.Therightsideofeachratwasusedasacontrol, and0.2gofsyntheticbonescaffoldwasimplantedintotheintramuscularpocketalone.Toseetheearlystagehealingthesampleswere harvested14daysaftertheimplantation,MSCsweredetectedbypositiveDAPIandMTCO2stainingin43%ofallthesamplesimplanted withMSCs,andnoinflammationsignswerepresentinanyimplantedanimal.Newvesselscouldbefoundinbothgroupsimplantedwith MSCs,butnotinthecontrolgroupofanimals.However,hematoxylin-eosinstainingcouldnotdetectnewlycreatedbonewithinthe implantinanyofthegroups.TheseresultswereinlinewithCOLL1staining,wherewecoulddetectpositivestainingonlyinthreecases,all ofwhichwereimplantedwithun-differentiatedMSCs.Accordingtoourfindings,therewerenobenefitsofusingthepre-differentiatedof MSC.

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Three-Dimensional Co-Cultures of Osteoblasts and Endothelial Cells in DegraPol Foam: Histological and High-Field Magnetic Resonance Imaging Analyses of Pre-Engineered Capillary Networks in Bone Grafts

Cited by 44 publications

References 27 publications

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis

Contribution of Endothelial Cells to Human Bone-Derived Cells Expansion in Coculture

The Osteogenic Potential of Human Nondifferentiated and Pre-differentiated Mesenchymal Stem Cells Combined with an Osteoconductive Scaffold – Early Stage Healing

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