Three-dimensional decellularized amnion membrane scaffold as a novel tool for cancer research; cell behavior, drug resistance and cancer stem cell content

Ganjibakhsh, Meysam; Mehraein, Fereshteh; Koruji, Morteza; Aflatoonian, Reza; Farzaneh, Parvaneh

doi:10.1016/j.msec.2019.02.090

Cited by 23 publications

(18 citation statements)

References 62 publications

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“…greater extent. These results are in a good agreement with other reports which indicate that scaffolds made of decellularized amniotic membrane, did not exhibit cytotoxicity [64].…”

Section: Mtt Assaysupporting

confidence: 93%

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Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

et al. 2020

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Decellularized membranes (DM) were obtained from bovine amniotic membranes (BAM) using four different decellularization protocols, based on physical, chemical, and mechanical treatment. The new material was used as a biological scaffold for in vitro skin cell culture. The DM were characterized using hematoxylin-eosin assay, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR-ATR), and differential scanning calorimetry (DSC). The in vitro cytotoxicity of DM was evaluated using MTT. The efficacy of decellularization process was assessed through DNA quantification and electrophoresis. All the used protocols showed a high effectiveness in terms of elimination of native cells, confirmed by DNA extraction and quantification, electrophoresis, and SEM, although protocol IV removes the cellular contents and preserve the native extracellular matrix (ECM) architecture which it can be considered as the most effective in terms of decellularization. FTIR-ATR and DSC on the other hand, revealed the effects of decellularization on the biochemical composition of the matrices. There was no cytotoxicity and the biological matrices obtained were a source of collagen for recellularization. The matrices of protocols I, II, and III were degraded at day 21 of cell culture, forming a gel. The biocompatibility in vitro was demonstrated; hence these matrices may be deemed as potential scaffold for epithelial tissue regeneration.

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“…greater extent. These results are in a good agreement with other reports which indicate that scaffolds made of decellularized amniotic membrane, did not exhibit cytotoxicity [64].…”

Section: Mtt Assaysupporting

confidence: 93%

“…DM of protocol IV exhibited a visible increase in metabolic activity, associated to the fact that it retained its biochemical properties to a greater extent. These results are in a good agreement with other reports which indicate that scaffolds made of decellularized amniotic membrane, did not exhibit cytotoxicity [64].…”

Section: Mtt Assaysupporting

confidence: 93%

Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

et al. 2020

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“…Nanofiber-based cell culture scaffolds sufficiently mimic the structural morphology and architecture of native extracellular matrix (ECM) and are therefore capable of recapitulating the intricacies of natural tissue microenvironment (Jiang et al 2012). These scaffolds are known to offer more biomimicking topographical cues that facilitate cell attachment, ease cell spreading and promote cell proliferation and differentiation (Gholami et al 2018a;Ganjibakhsh et al 2019). In addition, the porous structure of 3D culture systems provides more space for seeded cells and allows cell reorientation and tissue remodeling (Vacanti and Langer 1999).…”

Section: Introductionmentioning

confidence: 99%

Differentiation of neonate mouse spermatogonial stem cells on three-dimensional agar/polyvinyl alcohol nanofiber scaffold

Kashani

Bagher

Asgari

et al. 2020

Systems Biology in Reproductive Medicine

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Electrospun nanofiber matrices sufficiently mimic the structural morphology of natural extracellular matrix. In this study, we aimed to examine the effects of agar/polyvinyl alcohol nanofiber (PVA) scaffold on the proliferation efficiency and differentiation potential of neonate mouse spermatogonial stem cells (SCCs). Testicular cells were isolated from testes of 40 mouse pups and were seeded in: 1) 2D cell culture plates in the absence (2D/−GF) or presence (2D/+GF) of growth factors and 2) onto agar/PVA scaffold in the absence (3D/−GF) or presence (3D/+GF) of growth factors. The cells were subsequently cultured for 4 weeks. First 2 weeks were dedicated to proliferative phase, whereas the next 2 weeks emphasized the differentiation phase. The identity of the SCCs was investigated at different time-points by flow cytometry and quantitative reverse transcription PCR (qRT-PCR) analyses against the germ cell markers, including PLZF, Id-4, Gfrα-1, Tekt-1, and Sycp-3. After 2 weeks of culture, the 3D/+GF group showed the highest percentage of PLZF-positive cells among culture systems (P < 0.05). The expression levels of premeiotic markers (Id-4 and Gfrα-1) decreased significantly in all groups, particularly in 3D/+GF group after 28 days of culture. Additionally, the cells in the 3D/+GF group displayed the highest expression of meiotic (Sycp-3) and post-meiotic markers (Tekt-1) 14 days after differentiation induction. Seemingly, the combination of the agar/PVA scaffold and growth factor-supplemented medium synergistically increased the differentiation rate of mouse SSCs into meiotic and post-meiotic cells. Thus, agar/PVA nanofiber scaffolds may have the potential for applications in the restoration of infertility, especially in azoospermic males.

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“…A previous study showed that TNBCs are enriched for cancer stem cells [26]. Accumulating evidence has suggested that CSCs are related to tumor recurrence and drug resistance [27, 28]. Thus, targeting CSCs may provide a useful approach in controlling tumor resistance and lower relapse [29].…”

Section: Discussionmentioning

confidence: 99%

Gomisin M2 from Baizuan suppresses breast cancer stem cell proliferation in a zebrafish xenograft model

Yang

Hao

Pan

et al. 2019

Aging

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Gomisin M2 isolated from Schisandra viridis A. C. Smith has potential anti-tumor effects on certain cancers, including breast cancer. However, only a few investigations have been conducted on the effects of Gomisin M2 on breast cancer stem cells (CSCs), which have the ability to self-renew and differentiate, as a possible strategy to resolve cancer cell resistance to apoptosis and to improve treatments. It is essential to investigate the effects of Gomisin M2 on breast cancer stem cells (BCSCs). In this study, we enriched breast cancer stem cells with CD44+/CD24- from MDA-MB-231 and HCC1806 cells through magnetic-activated cell sorting and cultured these in serum-free medium. The ability of Gomisin M2 to kill breast cancer stem cells was evaluated in vitro and in vivo. Gomisin M2 significantly inhibited the proliferation of the triple-negative breast cancer cell lines and mammosphere formation in breast CSCs and downregulated the Wnt/β-catenin self-renewal pathway. Moreover, Gomisin M2 induced apoptosis and blocked the mitochondrial membrane potential of BCSCs. Gomisin M2 suppressed the proliferation of MDA-MB-231 and HCC1806 xenografts in zebrafish. Together, these findings suggest that the anti-BCSC activity of Gomisin M2 could become a promising starting point for the discovery of novel BCSC-targeting drugs.

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Three-dimensional decellularized amnion membrane scaffold as a novel tool for cancer research; cell behavior, drug resistance and cancer stem cell content

Cited by 23 publications

References 62 publications

Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

Differentiation of neonate mouse spermatogonial stem cells on three-dimensional agar/polyvinyl alcohol nanofiber scaffold

Gomisin M2 from Baizuan suppresses breast cancer stem cell proliferation in a zebrafish xenograft model

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