Three-Dimensional Human Neural Stem Cell Models to Mimic Heparan Sulfate Proteoglycans and the Neural Niche

Peall, Ian W.; Okolicsanyi, Rachel K.; Griffiths, Lyn R.; Haupt, Larisa M.

doi:10.1055/s-0041-1724117

Cited by 6 publications

(4 citation statements)

References 60 publications

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“…They also act as membrane-bound receptors. These molecules include heparan sulfate proteoglycans (HSPGs), comprising two distinct families: syndecans (4 members) and glypicans (6 members) [ 99 , 100 ]. Numerous articles give detailed descriptions of the structure and functioning of HSPGs [ 99 , 101 – 106 ].…”

Section: Ecm Receptor Signaling Pathways During Msc Differentiationmentioning

confidence: 99%

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Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells

Novoseletskaya,

Evdokimov,

Efimenko

2023

Cell Commun Signal

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The extracellular matrix (ECM) is a crucial component of the stem cell microenvironment, or stem-cell niches, and contributes to the regulation of cell behavior and fate. Accumulating evidence indicates that different types of stem cells possess a large variety of molecules responsible for interactions with the ECM, mediating specific epigenetic rearrangements and corresponding changes in transcriptome profile. Signals from the ECM are crucial at all stages of ontogenesis, including embryonic and postnatal development, as well as tissue renewal and repair. The ECM could regulate stem cell transition from a quiescent state to readiness to perceive the signals of differentiation induction (competence) and the transition between different stages of differentiation (commitment). Currently, to unveil the complex networks of cellular signaling from the ECM, multiple approaches including screening methods, the analysis of the cell matrixome, and the creation of predictive networks of protein–protein interactions based on experimental data are used. In this review, we consider the existing evidence regarded the contribution of ECM-induced intracellular signaling pathways into the regulation of stem cell differentiation focusing on mesenchymal stem/stromal cells (MSCs) as well-studied type of postnatal stem cells totally depended on signals from ECM. Furthermore, we propose a system biology-based approach for the prediction of ECM-mediated signal transduction pathways in target cells.

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Section: Ecm Receptor Signaling Pathways During Msc Differentiationmentioning

confidence: 99%

“…Glypicans are globular glycosylphophatidylinositol (GPI) anchored proteins [ 99 , 111 ]. Typically, glypicans have an N-terminal cysteine-rich domain similar to that of Frizzled receptors and mediate Wnt binding [ 101 ].…”

Section: Ecm Receptor Signaling Pathways During Msc Differentiationmentioning

confidence: 99%

Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells

Novoseletskaya,

Evdokimov,

Efimenko

2023

Cell Commun Signal

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“…Human neural stem cell models have revealed additional roles for heparin sulfate proteoglycans in proliferation, migration, differentiation, and cell survival in the inner ear and neural niche. 82 …”

Section: Inner Ear Structures and Cell-based Modelsmentioning

confidence: 99%

Recent advancements in cell-based models for auditory disorders

et al. 2022

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Introduction: Cell-based models play an important role in understanding the pathophysiology and etiology of auditory disorders. For the auditory system, models have primarily focused on restoring inner and outer hair cells. However, they have largely underrepresented the surrounding structures and cells that support the function of the hair cells. Methods: In this article, we will review recent advancements in the evolution of cell-based models of auditory disorders in their progression towards three dimensional (3D) models and organoids that more closely mimic the pathophysiology in vivo. Results: With the elucidation of the molecular targets and transcription factors required to generate diverse cell lines of the components of inner ear, research is starting to progress from two dimensional (2D) models to a greater 3D approach. Of note, the 3D models of the inner ear, including organoids, are relatively new and emerging in the field. As 3D models of the inner ear continue to evolve in complexity, their role in modeling disease will grow as they bridge the gap between cell culture and in vivo models. Conclusion: Using 3D cell models to understand the etiology and molecular mechanisms underlying auditory disorders holds great potential for developing more targeted and effective novel therapeutics.

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“…Peall et al thus present an overview on the current knowledge in three-dimensional human neural stem cell models, discussing their application in exploring HSPGs and the neural extracellular niche. 8 Better understanding of the role of HSPGs in neurologic pathologies could advance therapeutic approaches. Moreover, these models might be applicable to HS research on other stem cell types and their extracellular niche.…”

Section: Hspgs As Biomarkers and Treatment Targetsmentioning

confidence: 99%