Three‐Dimensional Interdigitated Electrode Array as a Tool for Surface Reactions Registration

Bratov, Andrey; Abramova, Natalia; Marco, M.‐Pilar; Sánchez‐Baeza, Francisco

doi:10.1002/elan.201100392

Cited by 18 publications

(21 citation statements)

References 20 publications

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“…Owing to its specific design, when an AC potential current is applied between the electrodes of a 3D-IDEA, the main portion of the current flows not through the surrounding solution but close to the surface of the barriers that form a microcapillary over each electrode digit. Changes in the electrical charge distribution at the solid/liquid interface provoke respective changes in conductivity along the barrier surface which is registered by the device (Bratov and Abramova, 2013;Bratov et al, 2012). Therefore, the results were in agreement with previous reported studies (Bratov et al, 2008a(Bratov et al, , 2008b, and 3D-IDEA were chosen as the transducer element in the following experiments.…”

Section: Optimization Of Peptide Immobilization By Eissupporting

confidence: 52%

“…Conventional IDEA sensors are planar devices with a flat sensor surface. As it was previously reported (Bratov et al, 2012(Bratov et al, , 2008a) three-dimensional IDEA devices (3D-IDEA), with insulating barriers separating electrode digits, permit to enhance considerably the transducer sensitivity. This translates into a higher capacity to monitor changes in the surface charge when a target molecule binds to the sensor.…”

Section: Introductionmentioning

confidence: 92%

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Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Hoyos‐Nogués

Brosel-Oliu

Abramova

et al. 2016

Biosensors and Bioelectronics

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Peri-implantitis, an inflammation caused by biofilm formation, constitutes a major cause of implant failure in dentistry. Thus, the detection of bacteria at the early steps of biofilm growth represents a powerful strategy to prevent implant-related infections. In this regard, antimicrobial peptides (AMPs) can be used as effective biological recognition elements to selectively detect the presence of bacteria. Thus, the aim of the present study was to combine the use of miniaturized and integrated impedimetric transducers and AMPs to obtain biosensors with high sensitivity to monitor bacterial colonization. Streptococcus sanguinis, which is one of the most prevalent strains in the onset of periodontal diseases, was used as a model of oral bacteria. To this end, a potent AMP derived from human lactoferrin was synthesized and covalently immobilized on interdigitated electrode arrays (IDEA). X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectroscopy (EIS) were employed to optimize and characterize the method of immobilization. Noteworthy, the interaction of Streptococcus sanguinis with AMP-coated sensors provoked significant changes in the impedance spectra, which were univocally associated with the presence of bacteria, proving the feasibility of our method. In this regard, the developed biosensor permits to detect the presence of bacteria at concentrations starting from 10(1) colony forming units (CFU)mL(-1) in KCl and from 10(2) CFUmL(-1) in artificial saliva. Moreover, the system was devoid of cytotoxicity for human fibroblasts. These results indicate that the proposed approach can be effective in the detection of initial stages of biofilm formation, and may be useful in the early prevention and treatment of peri-implantitis.

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Section: Optimization Of Peptide Immobilization By Eissupporting

confidence: 52%

Section: Introductionmentioning

confidence: 92%

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Hoyos‐Nogués

Brosel-Oliu

Abramova

et al. 2016

Biosensors and Bioelectronics

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“…This effect, in principle, may be used to control changes in surface charging at liquid/solid interfaces due to adsorption or surface chemical reactions, as we have recently shown. 24 As an example, experimental results for the adsorption of PEI on a SiO 2 device are presented in Figure 4. Preliminary experiments have shown that the adsorption of PEI on SiO 2 is very fast (saturation is reached in less than 5 min) and nearly irreversible.…”

Section: ¹1mentioning

confidence: 99%

A New Microcapillary Device for Controlling Surface Conductivity Changes at Solid–Liquid Interface

Bratov

Abramova

2012

Chemistry Letters

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A new microcapillary device based on an interdigitated electrode array is presented. Microcapillaries with metal electrodes at their bottom are formed as trenches in SiO 2 and are open at the top. In an electrolyte solution, the ac current applied to electrodes flows from one capillary to another and is significantly affected by changes in surface conductance at the SiO 2 /electrolyte interface. The effect of electrostatically assembled charged polyelectrolyte layers on the surface conductance is studied.Surface charges at a solid/liquid interface play an important role in many systems, but their effect becomes more important at the micro-or nanoscale.1,2 For example, fluid flow control and sample transport in lab-on-a-chip devices depend strongly on the surface charges on the channel walls.3 Another important field is the design of biomedical materials, as charge formation at a biopolymer/liquid interface is of the highest importance in their applications. 4 To study the charges at insulator/electrolyte solution interfaces, conventional electrokinetic methods are most commonly used, among which are streaming current or streaming potential methods and electroosmosis. Experiments are usually performed on macroscopic surfaces with a simple geometry, such as capillaries or microchannels.3,5 However, it must be noted that the interpretation of electrokinetic measurement results is not straightforward and a lack of adequate experimental methods that permit the direct analysis of electrical charging at solid/liquid interfaces is recognized by specialists in the field.When a tangential electric field is applied at a solid/solution interface, owing to a higher concentration of ions within the electrical double layer (EDL), the local electric current can be higher than the typical current in the bulk electrolyte solution. For a long time, it was considered that surface conductivity is associated only with the migration of ions accumulated within the diffuse part of the EDL. However, accumulated experimental evidence facilitated the revision of the EDL model for a solid/ liquid interface 6,7 and led to the assumption that under an electric potential gradient, the ions that can migrate under the electric field are located in a stagnant layer of the EDL. Thus, the overall surface conductivity is due to the migration of ions confined within a stagnant layer and due to ions accumulated in a mobile region of the diffuse part of the EDL. The current density in both surface layers is determined by the concentration and mobility of ions.We have previously reported a three-dimensional impedimetric transducer 8,9 that, when in contact with an electrolyte solution, is highly sensitive to the charges present on its surface. In this work, the possibility of this device monitoring changes in the surface conductivity at a solid/liquid interface is studied.The device was fabricated using conventional microelectronic fabrication techniques. A silicon wafer covered with a 2500-nm-thick thermally grown SiO 2 layer was used as the substrate....

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“…Several years ago the concept of a threedimensional interdigitated electrode arrays (3D-IDEA) with electrode fingers separated by an insulating barrier was proposed [9,17]. The specific design of this sensor structure allows for enhancement of its sensitivity for biochemical reactions taking place at the sensor surface [18].…”

Section: Introductionmentioning

confidence: 99%

“…Broad applicability of 3D-IDEA sensors to study interactions of biomolecules [18] can be useful to apply this sensor type for investigation of oxidoreductases. A synergism of 3D-IDEA and unique selectivity of oxidoreductases can be promising to design label-free ultrasensitive new 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 5 impedimetric biosensor, based on determination of the changes only in the electrical properties of immobilised enzyme layer and enzyme immobilisation matrix.…”

Section: Introductionmentioning

confidence: 99%

Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications

Voitechovič

Bratov

Abramova

et al. 2015

Electrochimica Acta

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Three‐Dimensional Interdigitated Electrode Array as a Tool for Surface Reactions Registration

Cited by 18 publications

References 20 publications

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

A New Microcapillary Device for Controlling Surface Conductivity Changes at Solid–Liquid Interface

Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications

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