Three-dimensional microscopy with phase-shifting digital holography

Zhang, Tong; Yamaguchi, Ichirou

doi:10.1364/ol.23.001221

Cited by 465 publications

(211 citation statements)

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“…Hence the technique finds applications in many different areas, including microscopy [137], surface shape measurement [138], and color holography [139]. PSDH can be applied with Fresnel configurations for macroscopic imaging [24] or image plane configuration for microscopy [98].…”

Section: Phase-shifting Digital Holographymentioning

confidence: 99%

Principles and techniques of digital holographic microscopy

2010

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Abstract. Digital holography is an emerging field of new paradigm in general imaging applications. We present a review of a subset of the research and development activities in digital holography, with emphasis on microscopy techniques and applications. First, the basic results from the general theory of holography, based on the scalar diffraction theory, are summarized, and a general description of the digital holographic microscopy process is given, including quantitative phase microscopy. Several numerical diffraction methods are described and compared, and a number of representative configurations used in digital holography are described, including off-axis Fresnel, Fourier, image plane, in-line, Gabor, and phase-shifting digital holographies. Then we survey numerical techniques that give rise to unique capabilities of digital holography, including suppression of dc and twin image terms, pixel resolution control, optical phase unwrapping, aberration compensation, and others. A survey is also given of representative application areas, including biomedical microscopy, particle field holography, micrometrology, and holographic tomography, as well as some of the special techniques, such as holography of total internal reflection, optical scanning holography, digital interference holography, and heterodyne holography. The review is intended for students and new researchers interested in developing new techniques and exploring new applications of digital holography. C 2010 Society of Photo-Optical Instrumentation Engineers.

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Section: Phase-shifting Digital Holographymentioning

confidence: 99%

Principles and techniques of digital holographic microscopy

2010

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“…The digital holographic microscopy (DHM) brings two new capabilities to optical light microcopy: it dramatically increases the depth of investigation and it can also provide quantitative phase contrast imaging [11][12][13][14][15][16][17][18]. Therefore, those capabilities are of great interest to study particles and organisms in water in flow cells or in a water volume.…”

Section: Introductionmentioning

confidence: 99%

High throughput holographic imaging-in-flow for the analysis of a wide plankton size range

Yourassowsky¹,

Dubois²

2014

Opt. Express

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We developed a Digital Holographic Microscope (DHM) working with a partial coherent source specifically adapted to perform high throughput recording of holograms of plankton organisms in-flow, in a size range of 3µm-300µm, which is of importance for this kind of applications. This wide size range is achieved with the same flow cell and with the same microscope magnification. The DHM configuration combines a high magnification with a large field of view and provides high-resolution intensity and quantitative phase images refocusing on high sample flow rate. Specific algorithms were developed to detect and extract automatically the particles and organisms present in the samples in order to build holograms of each one that are used for holographic refocusing and quantitative phase contrast imaging. Experimental results are shown and discussed.

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“…Digital holographic microscopy (DHM) is an optical technique useful to retrieve quantitative information of microscopic objects with subwavelength axial accuracy (Haddad et al, 1992;Zhang-Yamaguchi, 1998;Pedrini et al, 1999;Ferraro et al, 2005). An important feature of DHM is its non-invasive character that allows the appropriate study of microorganisms in vivo (Car et al, 2004;Marquet et al, 2005;Rappaz et al, 2005Javidi et al, 2006;Charrière et al, 2006).…”

Section: Introductionmentioning

confidence: 99%