Three-dimensional modelling of the femur and humerus in adult male guinea pigs (guinea pig) with computed tomography and some biometric measurement values

Abstract

Background: Guinea pig is a species belonging to the Caviidae family of the Rodentia order and is frequently used in experimental studies. Biomedical imaging methods are used in the diagnosis and treatment of many diseases in medicine. Among these methods, computed tomography (CT) is one of the most important imaging methods. In this study, it was aimed to perform the three-dimensional (3D) modelling of the CT images, obtained from the humerus and femur in the guinea pigs, via the MIMICS programme, and to make some biometric measurements regarding the bones over these models. Materials and methods: In the present study, 12 male adult guinea pigs were used. The soft tissue on the humerus and femur bones of the guinea pigs was removed. After this procedure, CT images at a 0.5 mm-thickness were obtained from the animals. The images were recorded in DICOM format. Then, the reconstruction process was performed from the images by using the 3D modelling programme MIMICS ® 13.1. On the 3D model of the humerus and femur (right-left), volumes, surface areas and lengths as well as other biometric parameters were measured separately, and the values were recorded. In addition, measurements of the bones were made with the help of a digital calliper. Results: Among the parameters obtained from 3D models, a statistical difference was observed between the right and left cortical thicknesses of the femur from the measurements of calliper and the right and left humerus volumes (p < 0.05); whereas, no statistical difference was found in other parameters of both measurements (p > 0.05). Conclusions: It can be stated that CT and 3D modelling can be used for the measurement of some parameters in the long bones of the guinea pigs.

Comparative Morphology of the Humerus of Rabbit, Guinea Pig and Mongoose

“…This might be due to breed, species and food variation. Witkowska et al (2014) and Dayan et al (2019) reported the width of humerus 0.8-1.0 cm and 0.7-0.8 cm, respectively, in guinea pig, which coincides with the present finding (0.8 cm). These differences might be due to species variation.…”

“…The width from the medial to lateral side at the distal extremity of humerus was found to be largest in mongoose, followed by rabbit and guinea pig (Table 2). Similar observations regarding the width of the distal extremity of humerus were reported by Witkowska et al (2014) and Dayan et al (2019) in guinea pigs. On the contrary, Pathak et al (2017) reported higher values for the width at distal extremity in Indian Tiger.…”

Ultrasonography, Microcomputed Tomography, and Macroscopic Preparation in an Anatomical Study of the Thoracic Limb of the Golden-Headed Lion Tamarin (Leontopithecus chrysomelas)

“…Although computed tomography is more commonly used to diagnose musculoskeletal problems, it can also be used to assess the anatomy of the front limb. Micro-CT has previously been used to analyse the skeletal system of the thoracic limb in mammals, reptiles, and birds [47][48][49][50][51]. In normal anatomy, CT seems to be sufficient, but micro-CT can be very useful when assessing pathology or changes in normal anatomy.…”

Different effects of Wnt/β‐catenin activation and parathyroid hormone on diaphyseal and metaphyseal in the early phase of femur bone healing of mice

“…The CT scan around the defect and images in DICOM format were archived. Using the segmentation software (Mimics: Interactive Medical Image Control System, Version 19.0, Materialize Company, Belgium) installed on a personal computer, based on thresholding and/or the semi‐automated segmentation method, the CT images were processed as 3D visualization …”