Three dimensional printed polylactic acid-hydroxyapatite composite scaffolds for prefabricating vascularized tissue engineered bone: An in vivo bioreactor model

Hai-feng, Zhang; Mao, Xiyuan; Zhao, Deyu; Jiang, Wenbo; Du, Zijing; Li, Qingfeng; Jiang, Chengming; Han, Dong

doi:10.1038/s41598-017-14923-7

Cited by 74 publications

(83 citation statements)

References 58 publications

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“…Since thermoplastic polymers such as PCL, poly (lactic acid), and poly (lactic-co-glycolic acid) are generally biologically inert, inorganic fillers such as tricalcium phosphate and HAp are commonly used to create biocomposites for bone tissue applications. 31,32 However, the addition of such fillers modifies the rheological properties of the parent polymer matrix. In most cases, as reported in the literature, the addition of fillers increases the viscosity of the polymer melt due to their high surface area.…”

Section: Rheological Measurementsmentioning

confidence: 99%

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Gerdes

Mostafavi

Ramesh

et al. 2020

Tissue Engineering Part A

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Bone defects are common and, in many cases, challenging to treat. Tissue engineering is an interdisciplinary approach with promising potential for treating bone defects. Within tissue engineering, three-dimensional (3D) printing strategies have emerged as potent tools for scaffold fabrication. However, reproducibility and quality control are critical aspects limiting the translation of 3D printed scaffolds to clinical use, which remain to be addressed. To elucidate the factors that yield to the generation of defects in bioprinting and to achieve reproducible biomaterial printing, the objective of this article is to frame a systematic approach for optimizing and validating 3D printing of poly(caprolactone) (PCL)-hydroxyapatite (HAp) composite scaffolds. We delineate the effect of PCL-to-HAp ratio, print velocity, print temperature, and extrusion pressure on the architectural and mechanical properties of the 3D printed scaffold. Furthermore, we present an in situ image-based monitoring approach to quantify key quality-related aspects of constructs, such as the ability to deposit material consistently and print elementary shapes with fewer flaws. Our results show that small defects generated during the printing process have a significant role in lowering the mechanical properties of 3D printed polymeric scaffolds. In addition, the in vitro osteoinductivity of the fabricated scaffolds is demonstrated.

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Section: Rheological Measurementsmentioning

confidence: 99%

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Gerdes

Mostafavi

Ramesh

et al. 2020

Tissue Engineering Part A

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show abstract

“…PCL was the most used polymer as it was found in 29 articles . PLA was used in 13 studies and PLGA in 14 studies . Polyethylene glycol (PEG) was also used in six studies but not as major polymer: it was mixed with PLA, PCL, or with polyoxyethylene terephthalate and polybutylene terephthalate (PEOT/PBT) .…”

Section: Resultsmentioning

confidence: 99%

“…TCP was the most frequent and it was used in 32 studies . HA was the second most used mineral, in its non‐modified form in 25 studies, its carbonated form in two studies, its sintered form in one study or just as an apatite coating in two studies . In two cases, HA and TCP were mixed together to obtain biphasic calcium phosphate (BCP) .…”

Section: Resultsmentioning

confidence: 99%

3D printed polymer–mineral composite biomaterials for bone tissue engineering: Fabrication and characterization

et al. 2019

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Applications in additive manufacturing technologies for bone tissue engineering applications requires the development of new biomaterials formulations. Different three‐dimensional (3D) printing technologies can be used and polymers are commonly employed to fabricate 3D printed bone scaffolds. However, these materials used alone do not possess an effective osteopromotive potential for bone regeneration. A growing number of studies report the combination of polymers with minerals in order to improve their bioactivity. This review exposes the state‐of‐the‐art of existing 3D printed composite biomaterials combining polymers and minerals for bone tissue engineering. Characterization techniques to assess scaffold properties are also discussed. Several parameters must be considered to fabricate a 3D printed material for bone repair (3D printing method, type of polymer/mineral combination and ratio) because all of them affect final properties of the material. Each polymer and mineral has its own advantages and drawbacks and numerous composites are described in the literature. Each component of these composite materials brings specific properties and their combination can improve the biological integration of the 3D printed scaffold. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2579–2595, 2019.

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“…The 3D printer (Fochif Tech, China) served in our study is based on the melt-deposition system (MDS) as our previous studies [25,26]. Firstly, the overall structure of PLLA scaffold was accomplished by a computer-aided design (CAD) module.…”

Section: Methodsmentioning

confidence: 99%

“…The pore size and shape of scaffolds can be casually adjusted to meet the different requirements of biomechanical strength and cell adhesion [24]. In our previous studies, we have successfully constructed tissue-engineered bone by using 3D-printed polylactic acid (PLA)/hydroxyapatite (HA) composite scaffolds [25,26]. Biodegradable distraction devices are in line with the idea of constructing a biomimetic bone.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional-Printed Poly-L-Lactic Acid Scaffolds with Different Pore Sizes Influence Periosteal Distraction Osteogenesis of a Rabbit Skull

Zhao

Jiang

Wang³

et al. 2020

BioMed Research International

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The repair of bone defects is a big challenge in reconstructive surgery. Periosteal distraction osteogenesis (PDO), as a promising technique used for bone regeneration, forms a space between the periosteum and bone cortex to regenerate the new bone merely by distracting the periosteum. In order to investigate the influence of distractor framework on the PDO, we utilized three-dimensional (3D) printing technology to fabricate three kinds of poly-L-lactic acid (PLLA) scaffolds with different pore sizes in this study. The in vitro experiments showed that the customized PLLA scaffolds had different-sized microchannels with low toxicity, good biocompatibility, and enough mechanical strength. Then, we built up an in vivo bioreactor under the skull periosteum of New Zealand white rabbits. The distractors with different pore sizes all could satisfy the demand of periosteal distraction in the animal experiments. After 8 weeks of consolidation period, the quality and quantity of the newly formed bone were improved with the increasing pore sizes of the distractors. Moreover, the newly formed bone also displayed an increasing degree of vascularization. In conclusion, 3D printing technology could promote the innovation of PDO devices and fabricate optimized scaffolds with appropriate pore sizes, shapes, and structures. It would help us regenerate more functional tissue-engineered bone and provide new ideas for further clinical application of the PDO technique.

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Three dimensional printed polylactic acid-hydroxyapatite composite scaffolds for prefabricating vascularized tissue engineered bone: An in vivo bioreactor model

Cited by 74 publications

References 58 publications

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

Process–Structure–Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds

3D printed polymer–mineral composite biomaterials for bone tissue engineering: Fabrication and characterization

Three-Dimensional-Printed Poly-L-Lactic Acid Scaffolds with Different Pore Sizes Influence Periosteal Distraction Osteogenesis of a Rabbit Skull

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