Three dimensional refractive index imaging with differential interference contrast microscopy

Htet, A; Buckley, Jared; Kostyk, Piotr; Rodriguez, Braulio Macias; Phelan, Shelley A.; Xu, Min

doi:10.1117/12.910339

Cited by 3 publications

(2 citation statements)

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“…Thus, the optical path length becomes a viable tool for quantification of DNA content in cell nuclei. [8][9][10] Here, we report a microscopic approach for determining whether cells are actively proliferating or quiescent by way of the optical path length within the cells' nuclear regions measured by quantitative DIC microscopy at a wavelength of 500nm. Furthermore, we attempt to group a population of cells into either the pre-S-phase subpopulation, or the post-S-phase subpopulation by the optical path lengths over the nuclear regions.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle imaging with quantitative differential interference contrast microscopy

Kostyk

Phelan

2013

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

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We report a microscopic approach for determining cell cycle stages by measuring the nuclear optical path length (OPL) with quantitative differential interference contrast (DIC) microscopy. The approach is validated by the excellent agreement between the proportion of proliferating-to-quiescent cancerous breast epithelial cells obtained from DIC microscopy, and that from a standard immunofluorescence assay.

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Section: Introductionmentioning

confidence: 99%

Cell cycle imaging with quantitative differential interference contrast microscopy

Kostyk

Phelan

2013

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

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“…However, the resulting image of commercial DIC microscope cannot be used directly for quantitative analysis, as the image intensity is not linearly proportional to the phase information. Kou et al found that by taking a through-focus series of images and with the transport-of-intensity equation (TIE), quantitative phase image can be retrieved from DIC microscope images 5,9 . The method named as TI-DIC is robust and requires no or minimal hardware modifications.…”

Section: Introductionmentioning

confidence: 99%

Lung cancer diagnosis with quantitative DIC microscopy and support vector machine

et al. 2017

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Single-shot quantitative phase imaging with polarization differential interference contrast

Strassberg

Shevtsova

Kamel

et al. 2021

Applied Physics Letters

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We present single-shot quantitative phase imaging with polarization differential interference contrast (PDIC) for a differential interference contrast microscope which records the unfiltered Stokes vector of the differential interference pattern with a polarization camera. PDIC enables high spatial resolution phase imaging in real-time, applicable to either absorptive or transparent samples and integrates simply with epi-fluorescence imaging. An algorithm with total variation regularization is further introduced to solve the quantitative phase map from the partial derivative along one single axis, improving the accuracy and the image quality from the Fourier transform solution. After quantifying the accuracy of PDIC phase imaging with numerical simulations and phantom measurements, we demonstrate the biomedical applications by imaging the quantitative phase of both stained and unstained histological tissue sections and visualizing the fission yeast Schizosaccharomyces pombe's cytokinesis.

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Three dimensional refractive index imaging with differential interference contrast microscopy

Cited by 3 publications

References 0 publications

Cell cycle imaging with quantitative differential interference contrast microscopy

Cell cycle imaging with quantitative differential interference contrast microscopy

Lung cancer diagnosis with quantitative DIC microscopy and support vector machine

Single-shot quantitative phase imaging with polarization differential interference contrast

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