Three-dimensional scaffold-free microtissues engineered for cardiac repair

Patino-Guerrero, Alejandra; Veldhuizen, Jaimeson; Zhu, Wuqiang; Migrino, Raymond Q.; Nikkhah, Mehdi

doi:10.1039/d0tb01528h

Cited by 15 publications

(17 citation statements)

References 192 publications

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“…Cardiac scaffolds based on natural or synthetic biomaterials can mimic the environment of the extracellular matrix, releasing bioactive molecules. Thus, several types of injectable or implanted scaffolds are being proposed so far [ 55 , 56 , 57 ].…”

Section: Cardiovascular Diseases: An Overview Into Human and Veterina...mentioning

confidence: 99%

Bacterial Cellulose and ECM Hydrogels: An Innovative Approach for Cardiovascular Regenerative Medicine

Silva

Pantoja

Almeida

et al. 2022

IJMS

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Cardiovascular diseases are considered the leading cause of death in the world, accounting for approximately 85% of sudden death cases. In dogs and cats, sudden cardiac death occurs commonly, despite the scarcity of available pathophysiological and prevalence data. Conventional treatments are not able to treat injured myocardium. Despite advances in cardiac therapy in recent decades, transplantation remains the gold standard treatment for most heart diseases in humans. In veterinary medicine, therapy seeks to control clinical signs, delay the evolution of the disease and provide a better quality of life, although transplantation is the ideal treatment. Both human and veterinary medicine face major challenges regarding the transplantation process, although each area presents different realities. In this context, it is necessary to search for alternative methods that overcome the recovery deficiency of injured myocardial tissue. Application of biomaterials is one of the most innovative treatments for heart regeneration, involving the use of hydrogels from decellularized extracellular matrix, and their association with nanomaterials, such as alginate, chitosan, hyaluronic acid and gelatin. A promising material is bacterial cellulose hydrogel, due to its nanostructure and morphology being similar to collagen. Cellulose provides support and immobilization of cells, which can result in better cell adhesion, growth and proliferation, making it a safe and innovative material for cardiovascular repair.

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Section: Cardiovascular Diseases: An Overview Into Human and Veterina...mentioning

confidence: 99%

Bacterial Cellulose and ECM Hydrogels: An Innovative Approach for Cardiovascular Regenerative Medicine

Silva

Pantoja

Almeida

et al. 2022

IJMS

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“…non-adhesive culturing dish, hanging drop devices). 71 , 82 , 93–99 Compared with 2D culture, 3D spheroidal cultivation accelerates the structural maturation of iPSC-CMs with upregulated expression of cTnT, sarcomeric α-actinin, and sarcomere length. 98 The 3D spheroids also improve metabolic maturation at both the molecular and fluxome levels with downregulated glycolysis and lipid biosynthesis, and upregulated oxidative phosphorylation.…”

Section: Current Approaches and Technologies To Further Mature Ipsc-cmsmentioning

confidence: 99%

Bioengineering approaches to mature induced pluripotent stem cell-derived atrial cardiomyocytes to model atrial fibrillation

Brown

Han

et al. 2021

Exp Biol Med (Maywood)

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Induced pluripotent stem cells (iPSCs) serve as a robust platform to model several human arrhythmia syndromes including atrial fibrillation (AF). However, the structural, molecular, functional, and electrophysiological parameters of patient-specific iPSC-derived atrial cardiomyocytes (iPSC-aCMs) do not fully recapitulate the mature phenotype of their human adult counterparts. The use of physiologically inspired microenvironmental cues, such as postnatal factors, metabolic conditioning, extracellular matrix (ECM) modulation, electrical and mechanical stimulation, co-culture with non-parenchymal cells, and 3D culture techniques can help mimic natural atrial development and induce a more mature adult phenotype in iPSC-aCMs. Such advances will not only elucidate the underlying pathophysiological mechanisms of AF, but also identify and assess novel mechanism-based therapies towards supporting a more ‘personalized’ (i.e. patient-specific) approach to pharmacologic therapy of AF.

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“…Current studies on regenerative medicine are taking advantage of tissue engineering (TE) to develop functional cardiac muscle constructs to repair or replace damaged and injured cardiac muscle tissue. 5,6 To achieve this goal, TE uses biologically active factors, cells and scaffolds, with scaffolds being a key element in TE strategies since they can be designed to provide structural support, while giving the necessary biochemical and biophysical cues to cells in order to promote their growth and differentiation into the desired tissue. 7,8 Besides being biocompatible, an ideal scaffold for cardiac tissue engineering should possess the following requirements: it must (i) have a similar structure to the native cardiac tissue; (ii) possess suitable surface properties (surface charge, surface chemistry, surface topography and surface wettability) to promote cell attachment, proliferation and differentiation; and (iii) be mechanically stable and possess electrical and mechanical properties close to those of the native cardiac tissue to enable the propagation of electrical signals, essential for continuous and synchronized tissue contraction.…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid-based electroactive materials: a novel approach for cardiac tissue engineering strategies

et al. 2022

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Three-dimensional scaffold-free microtissues engineered for cardiac repair

Abstract: In this review article, we present the state-of-the-art approaches and recent advancements in the engineering of scaffold-free cardiac microtissues for myocardial repair.

Cited by 15 publications

References 192 publications

Bacterial Cellulose and ECM Hydrogels: An Innovative Approach for Cardiovascular Regenerative Medicine

Bacterial Cellulose and ECM Hydrogels: An Innovative Approach for Cardiovascular Regenerative Medicine

Bioengineering approaches to mature induced pluripotent stem cell-derived atrial cardiomyocytes to model atrial fibrillation

Ionic liquid-based electroactive materials: a novel approach for cardiac tissue engineering strategies

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