Three-dimensional self-powered DNA walking machine based on catalyzed hairpin assembly energy transfer strategy

Moving dynamics of a nanorobot with three DNA legs on nanopore-based tracks

“…However, we should note that the current simulation model has several limitations. First, the robot core used in this study is smaller and lighter than those used in experiments, such as gold nanoparticle, 19,39 CdS nanocrystal, 31 and streptavidin protein, 101 which may affect the quantitative results of the simulations. Whereas, as the CG model accurately reflects the key properties of the nanorobot, especially for the ssDNA chains, the simulation results are still qualitatively reliable.…”

“…5,6 The DNA molecule is one of the most important building blocks for constructing these nanodevices due to its easily programmable sequence and welldefined base pairing rule. [7][8][9] Various DNA-based nanostructures, such as DNA switches, 10,11 DNA tweezers, 12,13 DNA rotors, 14,15 DNA carriers, 16,17 and DNA walkers, 18,19 have been designed and shown promising potentials in applications, including biosensing, [20][21][22] diagnostics, [23][24][25][26][27] and therapeutics. [28][29][30] To achieve complex nanomechanical tasks, it is often necessary to combine the functions of multiple types of the DNA nanomachines.…”

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

“…5A). 85 The appearance of the target broke the metastable structure of the hairpin and active CHA reaction, which caused the signal molecule to move away from the AuNPs, resulting in fluorescence recovery while the falling target spontaneously approaches the adjacent hairpin and continues the same operation, causing exponential growth of the signal. This high-integration design increases the local concentration of the hairpin on AuNPs and reduces the uncertainty of CHA component collisions in homogeneous solutions.…”

Chemiluminescence resonance energy transfer-based multistage nucleic acid amplification circuits for MiRNA detection with low background

“…Programmable DNA nanostructures offer precise tools for controlling the quantity, placement, and proximity of mole-cules with great precision. [11][12][13][14] In addition, the horseradish peroxidase (HRP)-mimicking hemin/G-quadruplex (hemin/G4) DNAzyme has also been developed as a highly effective and robust catalyst for chemiluminescent systems. 15,16 For instance, DNA-based dynamic networks were created for the orthogonal control of the CRET process by DNAzyme-connected QDs.…”