Three Dimensional Visualization of Human Cardiac Conduction Tissue in Whole Heart Specimens by High-Resolution Phase-Contrast CT Imaging Using Synchrotron Radiation

Shinohara, Gen; Morita, Kiyozo; Hoshino, Masato; Tsukube, Takuro; Kaneko, Yukihiro; Morishita, Hiroyuki; Oshima, Yoshihiro; Matsuhisa, Hironori; Iwaki, Ryuma; Takahashi, Masashi; Matsuyama, Taka‐aki; Hara, Kazuhiro; Yagi, Naoto

doi:10.1177/2150135116675844

Cited by 35 publications

(27 citation statements)

References 22 publications

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“…Propagation-based X-PCI (PB-X-PCI) has been previously proposed for the assessment of detailed cardiac structure both in excised non-beating rodent 18–20 and post-mortem human hearts 21–23 . In addition, fetal, neonatal and infant hearts have been assessed using synchrotron grating interferometry (GI) 24,25 , an alternative quantitative phase contrast imaging method. However, this technique would result in more challenging multiscale acquisitions with higher instrumentation requirements.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Animal Models of Cardiovascular Disease using Multiscale X-Ray Phase Contrast Tomography

Dejea

Garcia-Canadilla

Cook

et al. 2019

Sci Rep

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Cardiovascular diseases (CVDs) affect the myocardium and vasculature, inducing remodelling of the heart from cellular to whole organ level. To assess their impact at micro and macroscopic level, multi-resolution imaging techniques that provide high quality images without sample alteration and in 3D are necessary: requirements not fulfilled by most of current methods. In this paper, we take advantage of the non-destructive time-efficient 3D multiscale capabilities of synchrotron Propagation-based X-Ray Phase Contrast Imaging (PB-X-PCI) to study a wide range of cardiac tissue characteristics in one healthy and three different diseased rat models. With a dedicated image processing pipeline, PB-X-PCI images are analysed in order to show its capability to assess different cardiac tissue components at both macroscopic and microscopic levels. The presented technique evaluates in detail the overall cardiac morphology, myocyte aggregate orientation, vasculature changes, fibrosis formation and nearly single cell arrangement. Our results agree with conventional histology and literature. This study demonstrates that synchrotron PB-X-PCI, combined with image processing tools, is a powerful technique for multi-resolution structural investigation of the heart ex-vivo . Therefore, the proposed approach can improve the understanding of the multiscale remodelling processes occurring in CVDs, and the comprehensive and fast assessment of future interventional approaches.

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Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Animal Models of Cardiovascular Disease using Multiscale X-Ray Phase Contrast Tomography

Dejea

Garcia-Canadilla

Cook

et al. 2019

Sci Rep

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show abstract

“…Other applications have leveraged the 10-mm spatial resolution and high soft-tissue contrast. These have included visualizing myocardial fiber orientation (98)(99)(100), identifying the cardiac conduction system (101), visualizing microscopic vascular damage (102), and performing cardiac material decomposition (103). These experimental techniques may enable noninvasive high-resolution imaging of high-risk plaque and identification of subtle myocardial abnormalities in patients with cardiac disease.…”

Section: Development Of Phase-contrast Ct Toward Clinical Cardiovascumentioning

confidence: 99%

Next-Generation Hardware Advances in CT: Cardiac Applications

Kwan¹,

Pourmorteza²,

Stutman

et al. 2021

Radiology

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“…We have now shown that, when using phase-contrast computed tomography, it is possible to demonstrate the precise location of the conduction axis relative to its surroundings without destroying or altering the properties of autopsied human heart specimens. 9,10 The aim of this study is, therefore, to visualize and measure the location of the axis in the setting of atrioventricular septal defect with common atrioventricular junction using this technique.…”

Section: Introductionmentioning

confidence: 99%

Reassessment of the Location of the Conduction System in Atrioventricular Septal Defect Using Phase-Contrast Computed Tomography

Yoshitake

Kaneko

Morita

et al. 2020

Seminars in Thoracic and Cardiovascular Surgery

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on behalf of the SPring 8 Cardiovascular Structure Analyzing Research Group 2 The location of the atrioventricular conduction axis in the setting of atrioventricular septal defect has previously been shown by histology and intraoperative recordings. We have now reassessed the arrangement using phasecontrast computed tomography, aiming to provide precise measurements so as to optimize future surgical repairs. We used the system based on an X-ray Talbot grating interferometer using the beamline BL20B2 in a SPring-8 synchrotron radiation facility available in Japan. We analyzed 18 specimens. The atrioventricular node was found within a nodal triangle 1.7 mm from the coronary sinus, with 95% confidence intervals from 1.45 to 2.0 millimeters. The depth of the node from the right atrial endocardium was 1.0 mm, with 95% confidence intervals from 0.73 to 1.34 mm. The overall length of the scooped-out ventricular septum was 30.8 mm, with 95% confidence intervals from 27.5 to 34.1 millimeters. The length from the inferior atrioventricular junction to the takeoff of the right bundle branch was 12.8 mm, with 95% confidence intervals from 11.12 to 14.38 mm, giving a ratio of 0.43 for the extent of the axis along the inferior septum, with 95% confidence intervals of 0.38À0.48. The length of the non-branching bundle was 6.6 mm, with 95% confidence intervals from 5.57 to 7.7 mm. The proportion of septum occupied by the non-branching bundle was 0.22, with 95% confidence intervals from 0.18 to 0.26. Our findings confirm previous histological studies, The reconstructed block showing the precise location of the conductionaxis. Central Message Use of phase contrast computed tomography revealed the precise location of the atrioventricular conduction axis in hearts with atrioventricular septal defect. Perspective Statement No precise measurements have been provided to guide the placement of sutures during

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Three Dimensional Visualization of Human Cardiac Conduction Tissue in Whole Heart Specimens by High-Resolution Phase-Contrast CT Imaging Using Synchrotron Radiation

Abstract: This is the first demonstration that visualization of the AV conduction axis within human whole heart specimens is feasible with PCCT.

Cited by 35 publications

References 22 publications

Comprehensive Analysis of Animal Models of Cardiovascular Disease using Multiscale X-Ray Phase Contrast Tomography

Comprehensive Analysis of Animal Models of Cardiovascular Disease using Multiscale X-Ray Phase Contrast Tomography

Next-Generation Hardware Advances in CT: Cardiac Applications

Reassessment of the Location of the Conduction System in Atrioventricular Septal Defect Using Phase-Contrast Computed Tomography

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