Three-dimensional visualization of microvessel architecture of whole-mount tissue by confocal microscopy

Dickie, Renee; Bachoo, Robert; Rupnick, Maria A.; Dallabrida, Susan M.; DeLoid, Glen M.; Laı̈, Jean Luc; DePinho, Ronald A.; Rogers, Rick A.

doi:10.1016/j.mvr.2006.05.003

Cited by 80 publications

(69 citation statements)

References 30 publications

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“…As shown by the UHS-OMAG results in Fig. 2(d), the typical longitudinal orientation of the individual capillaries within the superficial layer agrees well with the observations from the previous studies 25,26 that used the multiphoton or confocal microscopy where the fluorescent dyes have to be involved in imaging. With its depthresolved attributes, UHS-OMAG could produce volumetric images in which the microvessel networks within different layers can be easily segmented and visualized.…”

Section: Discussionsupporting

confidence: 89%

Ultrahigh sensitive optical microangiography reveals depth-resolved microcirculation and its longitudinal response to prolonged ischemic event within skeletal muscles in mice

et al. 2011

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Section: Discussionsupporting

confidence: 89%

Ultrahigh sensitive optical microangiography reveals depth-resolved microcirculation and its longitudinal response to prolonged ischemic event within skeletal muscles in mice

et al. 2011

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“…New original results obtained using the combination of optical clearing with the known optical visualisation methods, such as laser speckle-contrast imaging [34, 71,75,77,79], OCT [20,29,35,[59][60][61]65,69,80,82], microscopic imaging [23,24,83,84], ultra-microscopy [85][86][87], etc., demonstrated high potentiality of their mutual use not only for getting high-resolution structure and functional tissue images in vitro [72,73,[83][84][85][86][87], but also for optical imaging and diagnostics in vivo [25,34,45,50,59,61,65,68,70,71,[75][76][77][79][80][81]. Table 1 shows the increase of the light penetration depth, caused by clearing agents in some diagnostic methods.…”

Section: Immersion Clearingmentioning

confidence: 99%

“…[48, 49] basing on the Monte-Carlo modelling the possibility of increasing the human skin probing depth was considered theoretically for the first time using the method of reflectance CM with the reduction of spatial fluctuations of the refractive index of the outer skin layers by optical clearing. Later in the paper by Dickie et al [83] the experimental method was described that allowed visualisation of microvessels of different mouse tissues using the CM at the depth up to 1500 µm below the sample surface due to the optical clearing of thick slices of the tissue. The OCA for CM in Refs.…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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“…The sections were stained for cell nuclei using DAPI (blue), for macrophages using Rat-Anti-Mouse CD68-Alexa-647 (green), and for endothelial cells by intravenous injection of isolectin-Alexa-488 (green) 15 min before sacrifice. 56 Red fluorescence signal originated from the QDs for both the lipid-coated and the bare silica particles. Three fluorescence signals were imaged simultaneously each time, in the course of which the red QD signal and the blue DAPI signal were held constant, but the third signal was recorded with filter sets suited for either endothelial cell or macrophage related fluorescence.…”

mentioning

confidence: 99%

Improved Biocompatibility and Pharmacokinetics of Silica Nanoparticles by Means of a Lipid Coating: A Multimodality Investigation

et al. 2008

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Silica is a promising carrier material for nanoparticle-facilitated drug delivery, gene therapy, and molecular imaging. Understanding of their pharmacokinetics is important to resolve bioapplicability issues. Here we report an extensive study on bare and lipid-coated silica nanoparticles in mice. Results obtained by use of a wide variety of techniques (fluorescence imaging, inductively coupled plasma mass spectrometry, magnetic resonance imaging, confocal laser scanning microscopy, and transmission electron microscopy) showed that the lipid coating, which enables straightforward functionalization and introduction of multiple properties, increases bioapplicability and improves pharmacokinetics.

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Three-dimensional visualization of microvessel architecture of whole-mount tissue by confocal microscopy

Cited by 80 publications

References 30 publications

Ultrahigh sensitive optical microangiography reveals depth-resolved microcirculation and its longitudinal response to prolonged ischemic event within skeletal muscles in mice

Ultrahigh sensitive optical microangiography reveals depth-resolved microcirculation and its longitudinal response to prolonged ischemic event within skeletal muscles in mice

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Improved Biocompatibility and Pharmacokinetics of Silica Nanoparticles by Means of a Lipid Coating: A Multimodality Investigation

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