In this work, we developed a new measurement system which includes a Wireless Instrumented Sphere (WIS) and a Graphical User Interface (GUI) software, called Real Time Analysis (RTA). This system is able to acquire, process and visualize the three axis acceleration of the WIS allowing the identification and measurements of rotations, vibrations and impacts in real time. The aim of this instrument is to help the fruit producers to reduce food wasting and improve quality, especially in Brazil, one of the major agricultural countries in the world, whose losses could surpass 20% along the post-harvesting handling chain. Additionally, a data Post Processing Analysis software (PPA) provided of a video synchronization option was developed to determine the impact magnitude, position and even the cause of the impact itself (drop, fruit-to-sphere impact, etc.). Both GUIs presented graphics of the three axis acceleration vectors, acceleration magnitude and velocity, as well as the calculations of the number of impacts (peak detection), maximum, minimum and average impact magnitude. The WIS board was encapsulated in the middle of a spherical transparent polyurethane elastomer. It was also intended to be a small, simple, robust and low cost instrument. Its final diameter of approximately 63 mm, 160 g weight and 1.1 relative density. The RTA reduces the time for testing and is suitable for a fast feedback and allows the user to make adjustments in the experiment setup, packing system or even monitor any process along the post-harvesting handling chain, with an immediate response. The PPA with video synchronization option, proved to be a unique tool, relating the acceleration information with the video position.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-582) contains supplementary material, which is available to authorized users.