In this study, we optimized the design of functional running pants and innovated a stereoscopic seaming technology. The purpose of the study was to explore the thermal and humid comfort of the athletes during running by assessing the changes in skin temperature, surface temperature, and skin humidity of a subject during running. The results of the study suggest that during continuous incremental running speed exercise and sweating heavily, the surface temperature of the running pants and the skin temperature of the human body gradually approached before running, and remained basically in equilibrium. At 40 min, the skin humidity of a subject decreased significantly, and the sweat accumulated by the human body was absorbed and evaporated. The running pants in this study still exhibit better thermal conductivity and quick-drying properties as compared to the moisture-wicking and quick-drying running pants on the market. In addition, the running pants provide designed comfort that meets the needs of the exercising human body. They effectively address the problems such as abrupt lines, rubbing against the skin, and unsuited pattern. The application of moisture-absorbing and quick-drying fabric and the innovative 0.2 cm stereoscopic seaming technology creates pores between the separation line and the human body. This allows for effective airflow throughout the pants, promoting the evaporation of sweat from the legs, which can aid athletic performance to some extent. The results of the study confirm that the optimized running pants significantly improve human body comfort and offer practical advantages in terms of athletic performance.